Electron Spin Resonance Studies of Free Radicals in Gamma-Irradiated Golden Hamster Embryo Cells: Radical Formation at 77 and 295 K, and Radioprotective Effects of Vitamin C at 295 K

1993 ◽  
Vol 136 (3) ◽  
pp. 361 ◽  
Author(s):  
Toru Yoshimura ◽  
Kiyotaka Matsuno ◽  
Tetsuo Miyazaki ◽  
Keiji Suzuki ◽  
Masami Watanabe
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Vitamin C ◽  
Electron Spin ◽  
Golden Hamster ◽  
Radical Formation ◽  
Spin Resonance ◽  
Hamster Embryo Cells ◽  
Embryo Cells ◽  
Gamma Irradiated

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

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