Expression of Transfected Recombinant Oncogenes Increases Radiation Resistance of Clonal Hematopoietic and Fibroblast Cell Lines Selectively at Clinical Low Dose Rate

1990 ◽  
Vol 122 (1) ◽  
pp. 44 ◽  
Author(s):  
T. J. FitzGerald ◽  
Stephen Henault ◽  
Maryann Sakakeeny ◽  
Maria Alessandra Santucci ◽  
Jacalyn H. Pierce ◽  
...  
Keyword(s):  
Dose Rate ◽  
Cell Lines ◽  
Radiation Resistance ◽  
Low Dose ◽  
Fibroblast Cell ◽  
Low Dose Rate ◽  
Fibroblast Cell Lines

scholarly journals Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines

2004 ◽  
Vol 45 (3) ◽  
pp. 385-391 ◽  
Author(s):  
N. CASTRO KREDER ◽  
R. ten CATE ◽  
H. M. RODERMOND ◽  
C. van BREE ◽  
N. A. P. FRANKEN ◽  
...  
Keyword(s):  
Dose Rate ◽  
Cell Lines ◽  
Cellular Response ◽  
Low Dose ◽  
Mutant Cell ◽  
X Ray ◽  
Low Dose Rate

Equivalence of Pulsed-Dose-Rate to Low-Dose-Rate Irradiation in Tumor and Normal Cell Lines

2001 ◽  
Vol 155 (2) ◽  
pp. 297-303 ◽  
Author(s):  
M. Niedbala ◽  
G. Alsbeih ◽  
C. E. Ng ◽  
G. P. Raaphorst
Keyword(s):  
Dose Rate ◽  
Cell Lines ◽  
Normal Cell ◽  
Low Dose ◽  
Low Dose Rate ◽  
Pulsed Dose Rate

SENSITIVITY OF HUMAN PROSTATIC CARCINOMA CELL LINES TO LOW DOSE RATE RADIATION EXPOSURE

1998 ◽  
Vol 159 (2) ◽  
pp. 591-598 ◽  
Author(s):  
Theodore L. Deweese ◽  
Jennifer M. Shipman ◽  
Larry E. Dillehay ◽  
William G. Nelson
Keyword(s):  
Radiation Exposure ◽  
Dose Rate ◽  
Cell Lines ◽  
Prostatic Carcinoma ◽  
Low Dose ◽  
Carcinoma Cell ◽  
Low Dose Rate ◽  
Carcinoma Cell Lines

scholarly journals Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Anna Acheva ◽  
Siamak Haghdoost ◽  
Alice Sollazzo ◽  
Virpi Launonen ◽  
Meerit Kämäräinen
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Dose Rate ◽  
Cell Lines ◽  
Stromal Cells ◽  
Epithelial To Mesenchymal Transition ◽  
Low Dose ◽  
Mesenchymal Transition ◽  
Low Dose Rate ◽  
Stromal Component

The aim of the study was to investigate the role of a microenvironment in the induction of epithelial-to-mesenchymal transition (EMT) as a sign of early stages of carcinogenesis in human lung epithelial cell lines after protracted low-dose rateγ-radiation exposures. BEAS-2B and HBEC-3KT lung cell lines were irradiated with low-dose rateγ-rays (137Cs, 1.4 or 14 mGy/h) to 0.1 or 1 Gy with or without adding TGF-β. TGF-β-treated samples were applied as positive EMT controls and tested in parallel to find out if the radiation has a potentiating effect on the EMT induction. To evaluate the effect of the stromal component, the epithelial cells were irradiated in cocultures with stromal MRC-9 lung fibroblasts. On day 3 post treatment, the EMT markers:α-SMA, vimentin, fibronectin, and E-cadherin, were analyzed. The oxidative stress levels were evaluated by 8-oxo-dG analysis in both epithelial and fibroblast cells. The protracted exposure to low Linear Energy Transfer (LET) radiation at the total absorbed dose of 1 Gy was able to induce changes suggestive of EMT. The results show that the presence of the stromal component and its signaling (TGF-β) in the cocultures enhances the EMT. Radiation had a minor cumulative effect on the TGF-β-induced EMT with both doses. The oxidative stress levels were higher than the background in both epithelial and stromal cells post chronic irradiation (0.1 and 1 Gy); as for the BEAS-2B cell line, the increase was statistically significant. We suggest that the induction of EMT in bronchial epithelial cells by radiation requires more than single acute exposure and the presence of stromal component might enhance the effect through free radical production and accumulation.

scholarly journals New Method for the Evaluation of the Response of Cultured Cell Lines to Continuous Low-dose-rate Irradiation from Encapsulated Iridium-192 Seeds with Hyperthermia

1997 ◽  
Vol 37 (4) ◽  
pp. 320-326
Author(s):  
Hisashi ADACHI ◽  
Kengo MATSUMOTO ◽  
Katsuzo KUNISHIO ◽  
Tomohisa FURUTA ◽  
Takashi OHMOTO
Keyword(s):  
Dose Rate ◽  
Cell Lines ◽  
Low Dose ◽  
New Method ◽  
Cultured Cell ◽  
Low Dose Rate ◽  
Iridium 192
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