The Effect of Local Hyperthermia on Nonproliferative, Compared with Proliferative, Epithelial Cells of the Mouse Intestinal Mucosa

1983 ◽  
Vol 94 (2) ◽  
pp. 252 ◽  
Author(s):  
S. P. Hume ◽  
J. C. L. Marigold ◽  
A. Michalowski
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Local Hyperthermia

scholarly journals Enterococcus faecium PNC01 isolated from the intestinal mucosa of chicken as an alternative for antibiotics to reduce feed conversion rate in broiler chickens

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yang He ◽  
Xuan Liu ◽  
Yuanyang Dong ◽  
Jiaqi Lei ◽  
Koichi Ito ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Conversion Rate ◽  
Broiler Chickens ◽  
Intestinal Epithelial Cells ◽  
Relative Length ◽  
Intestinal Epithelial ◽  
Feed Conversion ◽  
Broiler Production ◽  
Feed Conversion Rate

Abstract Background The development and utilization of probiotics had many environmental benefits for replacing antibiotics in animal production. Bacteria in the intestinal mucosa have better adhesion to the host intestinal epithelial cells compared to bacteria in the intestinal contents. In this study, lactic acid bacteria were isolated from the intestinal mucosa of broiler chickens and investigated as the substitution to antibiotic in broiler production. Results In addition to acid resistance, high temperature resistance, antimicrobial sensitivity tests, and intestinal epithelial cell adhesion, Enterococcus faecium PNC01 (E. faecium PNC01) was showed to be non-cytotoxic to epithelial cells. Draft genome sequence of E. faecium PNC01 predicted that it synthesized bacteriocin to perform probiotic functions and bacteriocin activity assay showed it inhibited Salmonella typhimurium from invading intestinal epithelial cells. Diet supplemented with E. faecium PNC01 increased the ileal villus height and crypt depth in broiler chickens, reduced the relative length of the cecum at day 21, and reduced the relative length of jejunum and ileum at day 42. Diet supplemented with E. faecium PNC01 increased the relative abundance of Firmicutes and Lactobacillus, decreased the relative abundance of Bacteroides in the cecal microbiota. Conclusion E. faecium PNC01 replaced antibiotics to reduce the feed conversion rate. Furthermore, E. faecium PNC01 improved intestinal morphology and altered the composition of microbiota in the cecum to reduce feed conversion rate. Thus, it can be used as an alternative for antibiotics in broiler production to avoid the adverse impact of antibiotics by altering the gut microbiota. Graphic Abstract

scholarly journals The transcription factor FoxM1 activates Nurr1 to promote intestinal regeneration after ischemia/reperfusion injury

2019 ◽  
Vol 51 (11) ◽  
pp. 1-12 ◽  
Author(s):  
Guo Zu ◽  
Jing Guo ◽  
Tingting Zhou ◽  
Ningwei Che ◽  
Baiying Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Intestinal Epithelial ◽  
Ki 67 ◽  
Intestinal Regeneration ◽  
Foxm1 Expression ◽  
Intestinal Ischemia Reperfusion

Abstract FoxM1 is involved in the regeneration of several organs after injury and expressed in the intestinal mucosa. The intrinsic mechanism of FoxM1 activity in the mucosa after intestinal ischemia/reperfusion (I/R) injury has not been reported. Therefore, we investigated the role of FoxM1 in mediating intestinal mucosa regeneration after I/R injury. Expression of FoxM1 and the proliferation of intestinal mucosa epithelial cells were examined in rats with intestinal I/R injury and an IEC-6 cell hypoxia/reperfusion (H/R) model. The effects of FoxM1 inhibition or activation on intestinal epithelial cell proliferation were measured. FoxM1 expression was consistent with the proliferation of intestinal epithelial cells in the intestinal mucosa after I/R injury. Inhibition of FoxM1 expression led to the downregulation of Ki-67 expression mediated by the inhibited expression of Nurr1, and FoxM1 overexpression promoted IEC-6 cell proliferation after H/R injury through activating Nurr1 expression. Furthermore, FoxM1 directly promoted the transcription of Nurr1 by directly binding the promoter of Nurr1. Further investigation showed low expression levels of FoxM1, Nurr1, and Ki-67 in the intestinal epithelium of patients with intestinal ischemic injury. FoxM1 acts as a critical regulator of intestinal regeneration after I/R injury by directly promoting the transcription of Nurr1. The FoxM1/Nurr1 signaling pathway represents a promising therapeutic target for intestinal I/R injury and related clinical diseases.

scholarly journals Adhesion Abilities of Lactobacillus Plantarum Strains Isolated from Nigerian Fermented Maize Food - Akamu

2017 ◽  
Vol 6 (5) ◽  
pp. 50
Author(s):  
Patience Chisa Obinna-Echem
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Ethyl Acetate ◽  
Lactobacillus Plantarum ◽  
Intestinal Mucosa ◽  
Haemolytic Activity ◽  
Cell Models ◽  
Polar Solvents ◽  
Probiotic Potential ◽  
General Order

Two strains of Lactobacillus plantarum isolated from akamu a Nigerian fermented maize food were investigated for probiotic potential based on: adhesion to hydrocarbons (hydrophobicity), porcine mucin and epithelial cell models. Gelatinase and haemolytic activities of the L. plantarum isolates were also studied. Adhesions to mono polar solvents (>22%) were significantly (p≤0.05) higher than the n-alkanes (<13%) with significant maximal affinity (35%) for chloroform an acidic solvent. The general order of affinity was chloroform > ethyl acetate > hexadecane > hexane. NGL7 had significantly (p≤0.05) the highest affinity for all the solvents. Both L. plantarum strains had significant adhesions to porcine mucin (≥6.51 Log10 CFU mL-1) after 2 h at 37oC. Viable counts on Caco-2 cells were 5.13 and 5.53 Log10 CFU mL-1 for NGL7 and NGL5 respectively. The L. plantarum strains possessed significant adhesion abilities: adhesion to hydrocarbons, porcine mucin and Caco-2 cells with no gelatinase and haemolytic activity. This suggested that the L. plantarum strains isolated from the Nigerian fermented maize food -akamu would be able to adhere to the intestinal mucosa and epithelial cells for beneficial health effects without posing any risk. 

The use of biochemical parameters for a qualitative and quantitative assessment of ischemic damage to the small-intestinal mucosa

1987 ◽  
Vol 7 (12) ◽  
pp. 917-923 ◽  
Author(s):  
Ludo Filez ◽  
Willy Stalmans ◽  
Freddy Penninkx ◽  
Raymond Kerremans ◽  
Karel Geboes
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Specific Activity ◽  
Small Intestinal Mucosa ◽  
Major Event ◽  
Qualitative And Quantitative ◽  
Histological Data ◽  
Small Intestinal ◽  
Good Agreement

Lactate dehydrogenase has been measured in the small-intestinal mucosa in order to assess its value as a marker for the effects of ischemia and of reperfusion. The decrease in specific activity of the enzyme illustrates the deleterious effect of reperfusion on the quality of the remaining epithelial cells. However, this parameter fails to detect the loss of epithelial cells, which is the major event during ischemia as well as during reperfusion. In contrast, the expression of enzyme activity per g protein of the underlying intestinal muscle allowed us, in addition, to assess quantitatively the loss of epithelial cells, in good agreement with the histological data.

P2Y6 Receptor Contributes to Neutrophil Recruitment to Inflamed Intestinal Mucosa by Increasing Cxc Chemokine Ligand 8 Expression in an AP-1-dependent Manner in Epithelial Cells

2012 ◽  
Vol 18 (8) ◽  
pp. 1456-1469 ◽  
Author(s):  
Djordje M. Grbic ◽  
Émilie Degagné ◽  
Jean-François Larrivée ◽  
Maude S. Bilodeau ◽  
Valérie Vinette ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Neutrophil Recruitment ◽  
Dependent Manner ◽  
Cxc Chemokine ◽  
Chemokine Ligand ◽  
P2y6 Receptor

Dose-Survival Characteristics of Epithelial Cells of Mouse Intestinal Mucosa

Radiology ◽  
1968 ◽  
Vol 91 (5) ◽  
pp. 998-1000 ◽  
Author(s):  
H. R. Withers ◽  
M. M. Elkind
Keyword(s):  
Epithelial Cells ◽  
Intestinal Mucosa

Species Specificity of In Vitro Escherichia coli Adherence to Host Intestinal Cell Membranes and Its Correlation with In Vitro Colonization and Infectivity

1980 ◽  
Vol 28 (3) ◽  
pp. 1019-1027 ◽  
Author(s):  
Christopher P. Cheney ◽  
Peter A. Schad ◽  
Samuel B. Formal ◽  
Edgar C. Boedeker
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Guinea Pigs ◽  
Species Specificity ◽  
Intestinal Cell ◽  
Resected Specimen ◽  
Brush Borders

We have previously described an in vitro assay for examining the mucosal adherence of a rabbit diarrheagenic Escherichia coli , RDEC-1. That assay defined the in vitro characteristics of RDEC-1 adherence to brush borders isolated from rabbit ileal epithelial cells. The present study was conducted to examine the species specificity of both in vitro RDEC-1 adherence and in vivo infectivity of RDEC-1 and to compare these specificities. Species specificity in vitro adherence was examined by using brush borders prepared from intestinal epithelial cells of rats, guinea pigs, and rabbits, as well as from a surgically resected specimen of human ileum. Strain RDEC-1 adherence to rabbit brush borders in vitro was significantly greater ( P < 0.001) than its adherence to brush borders from any of the other species. Regional specificity of in vitro adherence of RDEC-1 to ileal segments of rabbit intestinal mucosa was also demonstrated. There was significantly greater adherence of RDEC-1 to rabbit ileal brush borders as compared to rabbit jejunal brush borders ( P < 0.05). In vivo infectivity was assessed by inoculating RDEC-1 into rats, guinea pigs, and rabbits. RDEC-1 elicited diarrhea in all inoculated rabbits with the mean onset of illness occurring 5 days after inoculation. In contrast, none of the RDEC-1-inoculated rats or guinea pigs developed diarrhea. Furthermore, colonization studies in these animals revealed that RDEC-1 heavily colonized the ileum and cecum (10 9 RDEC-1 colony-forming units/g of tissue) of rabbits; however, only minimal colonization was observed in guinea pigs and rats. In conclusion, the correlation between in vitro adherence and in vivo infectivity that we have observed suggests that the presence of receptors, specific for bacteria, on the surface of the host intestinal mucosa determines species susceptibility to enteric colonization and infectivity by certain strains of enteropathogenic E. coli .

scholarly journals Immunocytochemical localization of ornithine transcarbamylase in rat intestinal mucosa. Light and electron microscopic study.

1988 ◽  
Vol 36 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Y Hamano ◽  
H Kodama ◽  
M Yanagisawa ◽  
Y Haraguchi ◽  
M Mori ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Light Microscopy ◽  
Intestinal Mucosa ◽  
Intestinal Epithelial Cells ◽  
Protein A ◽  
Intestinal Epithelial ◽  
Electron Microscopic ◽  
Small Intestinal

We investigated light and electron microscopic localization of ornithine transcarbamylase (OTC) in rat intestinal mucosa. In the immunoblotting assay of OTC-related protein, a single protein band with a molecular weight of about 36,500 is observed in extracts of liver and small intestinal mucosa but is not observed in those of stomach and large intestine. For light microscopy, tissue slices of the digestive system were embedded in Epon and stained by using anti-bovine OTC rabbit IgG and the immunoenzyme technique. For electron microscopy, slices of these and the liver tissues were embedded in Lowicryl K4M and stained by the protein A-gold technique. By light microscopy, the absorptive epithelial cells of duodenum, jejunum, and ileum stained positively for OTC, but stomach, large intestine, rectum, and propria mucosa of small intestine were not stained. Electron microscopy showed that gold particles representing the antigenic sites for OTC were confined to the mitochondrial matrix of hepatocytes and small intestinal epithelial cells. However, the enzyme was detected in mitochondria of neither liver endothelial cells, submucosal cells of small intestine, nor large intestinal epithelial cells. Labeling density of mitochondria in the absorptive epithelial cells of duodenum, jejunum, and ileum was about half of that in liver cells.

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