Permanence of Suppression of the Primary Immune Response in Rainbow Trout, Salmo gairdneri, Sublethally Exposed to Tritiated Water during Embryogenesis

1982 ◽  
Vol 91 (3) ◽  
pp. 533 ◽  
Author(s):  
John A. Strand ◽  
M. Paul Fujihara ◽  
Ted M. Poston ◽  
C. Scott Abernethy
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Tritiated Water ◽  
Primary Immune Response ◽  
Salmo Gairdneri

Suppression of the Primary Immune Response in Rainbow Trout, Salmo gairdneri, Sublethally Exposed to Tritiated Water During Embryogenesis

1977 ◽  
Vol 34 (9) ◽  
pp. 1293-1304 ◽  
Author(s):  
J. A. Strand ◽  
M. P. Fujihara ◽  
R. D. Burdett ◽  
T. M. Poston
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Total Protein ◽  
Serum Proteins ◽  
Tritiated Water ◽  
Primary Immune Response ◽  
Salmo Gairdneri ◽  
Major Protein ◽  
Antibody Synthesis ◽  
Agglutination Assay

Antibody synthesis, in response to vaccination with a 0.1-ml (1.8 × 108 cells/ml) intraperitoneally injected, heat-killed strain of Flexibacter columnaris, was employed to investigate the effect of tritium irradiation (0, 0.04, 0.4, 4.0, 40 rads total dose for 20 days during embryogenesis) on development of the primary immune response in 5-mo rainbow trout, Salmo gairdneri, reared under essentially pathogen-free conditions. Specific agglutinins to F. columnaris, determined 1-wk pre vaccination, and 3, 5, 7, 9, and 11 wk postvaccination increased rapidly in both control and irradiated fish following vaccination. Agglutinin levels in irradiated fish were suppressed to 50% of control levels at 40 rads during the 9th wk, and 50% of control levels at 4.0 rads during the 11th wk. Electrophoretic separation of serum proteins of both control and irradiated–vaccinated fish demonstrated four major protein fractions. Densitometry analyses demonstrated that fraction IV increased significantly in percent of total protein following antigenic stimulation, suggesting that fraction IV represents the specific humoral antibody to F. columnaris. The relative percent of total protein contained in fraction IV was significantly reduced in irradiated–vaccinated fish. Key words: antibody synthesis, agglutination assay, vaccination, Flexibacter columnaris, tritium, Salmo gairdneri, electrophoresis, serum protein, densitometry, primary immune response

Effect of Diet on the Immune Response of Rainbow Trout (Salmo gairdneri)

1984 ◽  
Vol 41 (8) ◽  
pp. 1244-1247 ◽  
Author(s):  
Vicki S. Blazer ◽  
Richard E. Wolke
Keyword(s):  
Immune Response ◽  
Rainbow Trout ◽  
Control Diet ◽  
Serum Antibody ◽  
Peritoneal Macrophages ◽  
Salmo Gairdneri ◽  
Yersinia Ruckeri ◽  
Nonspecific Resistance ◽  
Commercial Diet ◽  
T Cell Migration

To compare the effect of diet on the immune response of rainbow trout (Salmo gairdneri), we maintained fish on either a commercial feed or a laboratory prepared (control) diet for 12–16 wk before antigenic exposure. The immune response to two antigens, sheep red blood cells (SRBC) and Yersinia ruckeri, was assessed. Both T-cell (migration inhibition factor) and B-cell (plaque-forming cell) responses to SRBC were measured. The plaque-forming cell response and the humoral (serum antibody) responses to Y. ruckeri were assayed. In addition, the phagocytic ability of peritoneal macrophages was assessed as a nonspecific resistance factor. We found that fish maintained on the commercial diet had a significantly lower immune response when compared with fish fed the control diet, although all fish appeared healthy and suffered no mortalities throughout the experiment. We indicate the importance of considering the effects of diet on immunity and disease resistance in future feed formulations.

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