The Effect of Ionizing Radiation In Vitro upon Fetal Mouse Liver Erythroid Cells

1981 ◽  
Vol 88 (1) ◽  
pp. 79 ◽  
Author(s):  
M. R. Reddy ◽  
J. E. Fuhr
Keyword(s):  
Ionizing Radiation ◽  
Mouse Liver ◽  
Erythroid Cells ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

scholarly journals Comparison of the hemagglutination inhibition assay kit for erythropoietin (ESF) with the fetal mouse liver cell bioassay in vitro

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Liver Cell ◽  
Hemagglutination Inhibition ◽  
Mouse Liver ◽  
Normal Human Serum ◽  
Fetal Mouse ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
Normal Human

The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.

scholarly journals Erythroid regulatory factors: correlation of detectable titers in vivo and in vitro

Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1238-1242
Author(s):  
CD Dunn ◽  
RD Lange
Keyword(s):  
Liver Cell ◽  
Mouse Liver ◽  
Fetal Mouse ◽  
Regulatory Factors ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver

Titers of erythroid regulatory factors (ERF), as measured in vivo with a standard erythrocythemic mouse technique, showed a highly significant correlation with those obtained in vitro with a fetal mouse liver cell (FMLC) assay. The results from the assay in vitro were somewhat higher than would be predicted from the assay in vivo. Nevertheless, the FMLC technique has been found to be a valid and useful technique particularly in the study of serum ERF titers.

scholarly journals Comparison of the hemagglutination inhibition assay kit for erythropoietin (ESF) with the fetal mouse liver cell bioassay in vitro

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959 ◽  
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Liver Cell ◽  
Hemagglutination Inhibition ◽  
Mouse Liver ◽  
Normal Human Serum ◽  
Fetal Mouse ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
Normal Human

Abstract The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.

Polyamines as an Inhibitor on Erythropoiesis of Hemodialysis Patients by In Vitro Bioassay Using the Fetal Mouse Liver Assay

2006 ◽  
Vol 10 (3) ◽  
pp. 267-272 ◽  
Author(s):  
Katsunori Yoshida ◽  
Tatsuo Yoneda ◽  
Syouki Kimura ◽  
Kiyohide Fujimoto ◽  
Eigoro Okajima ◽  
...  
Keyword(s):  
Mouse Liver ◽  
Hemodialysis Patients ◽  
In Vitro Bioassay ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

In Vitro Assay of Erythropoietin in Fetal Mouse Liver Cultures.

1981 ◽  
Vol 47 (3) ◽  
pp. 469-478 ◽  
Author(s):  
N. C. Brandan ◽  
P. M. Cotes ◽  
J. Espada
Keyword(s):  
Mouse Liver ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

scholarly journals Erythroid regulatory factors: correlation of detectable titers in vivo and in vitro

Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1238-1242 ◽  
Author(s):  
CD Dunn ◽  
RD Lange
Keyword(s):  
Liver Cell ◽  
Mouse Liver ◽  
Fetal Mouse ◽  
Regulatory Factors ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver

Abstract Titers of erythroid regulatory factors (ERF), as measured in vivo with a standard erythrocythemic mouse technique, showed a highly significant correlation with those obtained in vitro with a fetal mouse liver cell (FMLC) assay. The results from the assay in vitro were somewhat higher than would be predicted from the assay in vivo. Nevertheless, the FMLC technique has been found to be a valid and useful technique particularly in the study of serum ERF titers.

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