The Effect of Irradiated Feeder Cells on the X-Ray Survival Curve Shape of Freshly Explanted Human Tumor Cells and a Standard Human Tumor Cell Line

1980 ◽  
Vol 81 (1) ◽  
pp. 150 ◽  
Author(s):  
J. Wells ◽  
R. J. Berry ◽  
A. H. Laing
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Survival Curve ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Curve Shape ◽  
Feeder Cells ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
X Ray

scholarly journals Ultrastructure of double minutes from a human tumor cell line.

1979 ◽  
Vol 83 (3) ◽  
pp. 663-666 ◽  
Author(s):  
P E Barker ◽  
E Stubblefield
Keyword(s):  
Electron Microscopy ◽  
Chemical Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
Double Minutes

Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.

scholarly journals siRNAs Targeting Mouse-Specific lncRNA AA388235 Induce Human Tumor Cell Pyroptosis/Apoptosis

2021 ◽  
Vol 11 ◽  
Author(s):  
Yan-Ru Chen ◽  
Wan-Ying Feng ◽  
Yuan-Xiong Cheng ◽  
Hao Zhu ◽  
Hong-Juan Liu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Large Scale ◽  
Human Tumor ◽  
Mouse Tumor ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
Therapeutic Drugs ◽  
Species Specific

Species-specific lncRNAs significantly determine species-specific functions through various ways, such as epigenetic regulation. However, there has been no study focusing on the role of species-specific lncRNAs in other species yet. Here, we found that siRNAs targeting mouse-specific lncRNA AA388235 could significantly induce death of human tumor cells, although it has no effect on mouse tumor cells and normal human cells. The mechanism studies showed that these siRNAs could activate the response of human tumor cells to exogenous nucleic acids, induce pyroptosis and apoptosis in the presence of GSDME, but induce apoptosis in the absence of GSDME. They also significantly inhibited the growth of human tumor cells in vivo. 17 siRNAs were designed for seven more mouse-specific lncRNAs selected randomly, among which 12 siRNAs targeting five lncRNAs induced death in human tumor cell. Our study not only demonstrates that the siRNAs designed for knocking down mouse-specific lncRNA AA388235 can be potential tumor therapeutic drugs, but also suggests that non-human species-specific lncRNAs are a huge potential library that can be used to design siRNAs for tumor treatment. Large-scale screening based on this is promising.

Pharmacological profiling of disulfiram using human tumor cell lines and human tumor cells from patients

2007 ◽  
Vol 73 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Malin Wickström ◽  
Katarina Danielsson ◽  
Linda Rickardson ◽  
Joachim Gullbo ◽  
Peter Nygren ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Human Tumor Cells ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines

Inhibition by dihydrorifampicin of RNA polymerases I and II isolated from nuclei of Rous sarcoma cells and human tumor cells HEp-2

1979 ◽  
Vol 44 (9) ◽  
pp. 2722-2736 ◽  
Author(s):  
Jindřich Kára ◽  
Zdeněk Hostomský
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Selective Inhibition ◽  
Rna Polymerases ◽  
Embryonic Cells ◽  
Human Tumor Cells ◽  
Ribonucleic Acids ◽  
Rous Sarcoma ◽  
Sarcoma Cells

Dihydrorifampicin, a rifampicin derivative hydrogenated at the 18-19 carbon atoms of the aliphatic ansa chain of the rifampicin molecule, inhibits the enzymatic activity of RNA polymerases I and II, isolated from the nuclei of avian tumor cells (Rous sarcoma) and from the human tumor cell line HEp-2. The RNA polymerases from these tumors have been separated and partially purified by chromatography on DEAE Sephadex A-25 and characterized by the sensitivity to α-amanitin. The [3H]UMP-labeled ribonucleic acids synthesized in the isolated nuclei of Rous sarcoma cells in the presence and absence of DHR were analyzed by sedimentation analysis in sucrose density gradients. It was found that the synthesis of rRNAs and mRNAs is very significantly inhibited by dihydrorifampicin, whereas the synthesis of tRNAs is much less inhibited at the same DHR concentration (100μg/ml). The observed cytostatic effect of DHR on the growth of human tumor cells HEp-2 and embryonic cells in culture is apparently mediated by the selective inhibition of RNA polymerases I and II in human and avian cells. The relationship between the molecular structure of DHR and its affinity to RNA polymerases of eukaryotic cells is discussed.

Generation and Characterization of a Low-Degree Drug-Resistant Human Tumor Cell Line

Oncology ◽  
1990 ◽  
Vol 47 (6) ◽  
pp. 488-494 ◽  
Author(s):  
Alessandra Mazzoni ◽  
Fabio Trave ◽  
Patrizia Russo ◽  
Angelo Nicolin ◽  
Youcef M. Rustum
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cell Line ◽  
Drug Resistant ◽  
Human Tumor Cell ◽  
Low Degree

Abstract 5075: Phenotypic human tumor cell line profiling and predictive biomarker analysis for epigenetic inhibitors

2017 ◽  
Author(s):  
Usha Warrior ◽  
Jesse J. Parry ◽  
Samantha C. Burkart ◽  
Lee R. Cavedine ◽  
Alyssa M. Cracchiolo ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Predictive Biomarker ◽  
Human Tumor Cell Line ◽  
Human Tumor Cell ◽  
Biomarker Analysis
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