Cessation of Respiration after Far-Ultraviolet Irradiation of Escherichia coli B/r: Loss of Unaltered Pyridine Nucleotides to the Medium

1979 ◽  
Vol 79 (3) ◽  
pp. 611 ◽  
Author(s):  
R. L. Schenley ◽  
P. A. Swenson ◽  
J. G. Joshi
Keyword(s):  
Escherichia Coli ◽  
Ultraviolet Irradiation ◽  
Pyridine Nucleotides ◽  
Far Ultraviolet ◽  
Escherichia Coli B

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

scholarly journals Separation and Characterization of Deoxyribonucleases of Escherichia coli B

1966 ◽  
Vol 241 (13) ◽  
pp. 3090-3096
Author(s):  
Sally E. Jorgensen ◽  
James F. Koerner
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli B
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