Susceptibility of X-Ray-Arrested HeLa S3 Cells to Additional Arrest

1976 ◽  
Vol 66 (3) ◽  
pp. 649 ◽  
Author(s):  
L. J. Tolmach ◽  
T. D. Griffiths ◽  
R. W. Jones
Keyword(s):  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

X-ray microanalysis of HeLa S3 cells. II. Analysis of elemental levels during the cell cycle

1983 ◽  
Vol 62 (1) ◽  
pp. 339-350
Author(s):  
A. Warley ◽  
J. Stephen ◽  
A. Hockaday ◽  
T.C. Appleton
Keyword(s):  
Cell Cycle ◽  
Monovalent Cation ◽  
The Other ◽  
G1 Phase ◽  
Sodium Potassium ◽  
X Ray ◽  
Cation Concentration ◽  
Cell Compartments ◽  
Hela S3 ◽  
Hela S3 Cells

HeLa S3 cells were synchronized using hydroxyurea. Cryoultramicrotomy and X-ray microanalysis were used to study changes occurring in concentrations of elements during the cell cycle of the synchronized cells. Three subcellular compartments were studied: cytoplasm, nucleus and nucleolus. Potassium concentrations showed little fluctuation in all of the cell compartments during the cell cycle. Sodium concentrations increased during S. and M phases, returning to lower levels in the G1 phase. Chlorine concentrations were highest during the S and G2 phases. At all stages of the cell cycle respective concentrations of potassium, sodium, sulphur and chlorine were similar in the cytoplasm and nucleus. Concentrations of phosphorus increased in the nucleus during S, G2 and M, and also showed fluctuations in the nucleolus during the cycle; these were not seen in the cytoplasm. In S, M and M/G1 sodium concentrations were highest in the nucleolus compared with the other compartments. In the cytoplasm these changes resulted in an increase in total monovalent cation concentration (i.e. sodium + potassium) during S, G2 and M, which returned to base levels after mitosis. This increase in monovalent cation concentration is due almost entirely to the increase in sodium, with little change occurring in the concentration of potassium.

scholarly journals Modification of X-Ray-Induced Killing of HeLa S3 Cells by Inhibitors of DNA Synthesis

1967 ◽  
Vol 7 (6) ◽  
pp. 779-795 ◽  
Author(s):  
Barbara G. Weiss ◽  
L.J. Tolmach
Keyword(s):  
Dna Synthesis ◽  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

X-ray microanalysis of HeLa S3 Cells. I. Instrumental calibration and analysis of randomly growing cultures

1983 ◽  
Vol 60 (1) ◽  
pp. 217-229
Author(s):  
A. Warley ◽  
J. Stephen ◽  
A. Hockaday ◽  
T.C. Appleton
Keyword(s):  
Cell Damage ◽  
Linear Calibration ◽  
Photometric Analysis ◽  
Sodium Potassium ◽  
X Ray ◽  
Potassium Tartrate ◽  
Freeze Dried ◽  
Hela S3 ◽  
Low K ◽  
Hela S3 Cells

Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Standardization was validated by comparing flame-photometric analysis of gelatin containing sodium potassium tartrate with that of X-ray microanalysis. Freeze-dried sections of cells showed good morphology and analysis of whole sections of the cells showed that K/Na varied in individual cells. Low K/Na could not be ascribed to cell damage or to the sequestering of Na in any particular subcompartment of the cells. Treatment with ouabain caused changes in levels of all the elements studied and resulted in a low K/Na ratio in all cells.

scholarly journals X-ray Sensitivity of HeLa S3 Cells in the G2 Phase

1967 ◽  
Vol 7 (1) ◽  
pp. 77-94 ◽  
Author(s):  
Bozidar Djordjevic ◽  
L.J. Tolmach
Keyword(s):  
X Ray ◽  
Hela S3 ◽  
G2 Phase ◽  
Hela S3 Cells

scholarly journals X-ray microanalysis of synchronized HeLa S3 cells infected with Vaccinia virus

1985 ◽  
Vol 75 (1) ◽  
pp. 401-409
Author(s):  
A. Warley ◽  
J. Stephen
Keyword(s):  
Protein Synthesis ◽  
Vaccinia Virus ◽  
Post Infection ◽  
Host Protein ◽  
Flame Photometry ◽  
G1 Phase ◽  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells ◽  
Host Protein Synthesis

X-ray microanalysis has been used to study changes in concentration of P, S, Cl Na and K in synchronized HeLa S3 cells in g1 phase infected with Vaccinia virus. No dramatic redistribution of elements between nucleus and cytoplasm was demonstrated during the first 6 h post-infection, during which time viral shut-off of host protein synthesis occurred. [Cl] increased relative to [Na] and [K] during the first 3 h post-infection. The magnitude and direction of change in [K] and [Na] (from approximately 10 cells/analysis) was compared with data previously obtained by flame photometry (from approximately 10(6) cells/analysis) and found to be remarkably parallel.

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