Effects of X Irradiation on Respiratory Control and P/O Ratio in Isolated Rat Liver Mitochondria Having Different Respiratory Control Ratios

1976 ◽  
Vol 66 (2) ◽  
pp. 427 ◽  
Author(s):  
Joe Atsuta ◽  
Shunzo Okajima
Keyword(s):  
Rat Liver ◽  
Respiratory Control ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
X Irradiation ◽  
Isolated Rat

scholarly journals Ki-Energy (Life-Energy) Protects Isolated Rat Liver Mitochondria from Oxidative Injury

2006 ◽  
Vol 3 (4) ◽  
pp. 475-482 ◽  
Author(s):  
Tsuyoshi Ohnishi ◽  
Tomoko Ohnishi ◽  
Kozo Nishino
Keyword(s):  
Heat Treatment ◽  
Rat Liver ◽  
Near Infrared ◽  
Respiratory Control ◽  
Thiobarbituric Acid ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Heat Treated ◽  
Isolated Rat

We investigated whether ‘Ki-energy’ (life-energy) has beneficial effects on mitochondria. The paradigm we developed was to keep isolated rat liver mitochondria in conditions in which they undergo heat deterioration (39°C for 10 min). After the heat treatment, the respiration of the mitochondria was measured using a Clarke-type oxygen electrode. Then, the respiratory control ratio (RC ratio; the ratio between State-3 and State-4 respiration, which is known to represent the integrity and intactness of isolated mitochondria) was calculated. Without the heat treatment, the RC ratio was >5 for NADH-linked respiration (with glutamate plus malate as substrates). The RC ratio decreased to 1.86–4.36 by the incubation at 39°C for 10 min. However, when Ki-energy was applied by a Japanese Ki-expert during the heat treatment, the ratio was improved to 2.24–5.23. We used five preparations from five different rats, and the significance of the differences of each experiment was eitherP< 0.05 orP< 0.01 (n= 3–5). We analyzed the degree of lipid peroxidation in the mitochondria by measuring the amount of TBARS (thiobarbituric acid reactive substances). The amount of TBARS in heat-treated, no Ki-exposed mitochondria was greater than that of the control (no heat-treated, no Ki-exposed). However, the amount was reduced in the heat-treated, Ki-exposed mitochondria (two experiments; bothP< 0.05) suggesting that Ki-energy protected mitochondria from oxidative stress. Calcium ions may play an important role in the protection by Ki-energy. Data also suggest that the observed Ki-effect involves, at least, near-infrared radiation (0.8–2.7 μm) from the human body.

Interaction of aromatic aldehydes with isolated rat liver mitochondria

1982 ◽  
Vol 31 (11) ◽  
pp. 2025-2029 ◽  
Author(s):  
Charles R. Wolf ◽  
Helen Harmon ◽  
Carol M. Schiller
Keyword(s):  
Rat Liver ◽  
Aromatic Aldehydes ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat
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