G 2 Block in Chinese Hamster Cells Induced by X-Irradiation, Hyperthermia, Cycloheximide, or Actinomycin-D

1976 ◽  
Vol 65 (3) ◽  
pp. 511 ◽  
Author(s):  
W. C. Dewey ◽  
D. P. Highfield
Keyword(s):  
Actinomycin D ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
X Irradiation

scholarly journals CELL KILLING BY ACTINOMYCIN D IN RELATION TO THE GROWTH CYCLE OF CHINESE HAMSTER CELLS

1969 ◽  
Vol 42 (2) ◽  
pp. 366-376 ◽  
Author(s):  
M. M. Elkind ◽  
E. Kano ◽  
H. Sutton-Gilbert
Keyword(s):  
Dna Synthesis ◽  
Functional Capacity ◽  
Response Pattern ◽  
Actinomycin D ◽  
Chinese Hamster ◽  
Growth Cycle ◽  
S Phase ◽  
Chinese Hamster Cells ◽  
X Irradiation ◽  
A Cell

Using Chinese hamster cells in culture, we have measured the effectiveness of actinomycin D to suppress division as a function of the position, or age, of a cell in its growth cycle. Cells were first exposed to millimolar concentrations of hydroxyurea in order to produce a synchronized population just before the onset of DNA synthesis. Thereafter, the survival response after 30 min exposures to actinomycin D was measured. Cells become resistant as they enter the S phase and then sensitive again in the latter part of S. When they reach G2 (or G2-mitosis) they are maximally resistant; at 1.0 µg/ml, for example, the survival in G2 is 30-fold greater than it is in G1. These results, plus measurements reported earlier on the interaction of damage in S cells due to actinomycin D and X-irradiation, suggest that the age-response pattern of the toxic effects of this drug probably reflects both the functional capacity of DNA-actinomycin complexes and the ability of this antibiotic to penetrate chromatin and bind to DNA.

Mutational Extinction Induced by Sequential X Irradiation and Actinomycin D Treatments in Chinese Hamster Ovary Cells

1986 ◽  
Vol 107 (2) ◽  
pp. 216
Author(s):  
N. Tokita ◽  
M. A. MacInnes ◽  
M. E. Wilder ◽  
D. J. Chen ◽  
S. G. Carpenter ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Actinomycin D ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
X Irradiation

Effects of actinomycin D and puromycin on colchicine-induced endoreduplication in chinese hamster cells in vitro

1976 ◽  
Vol 14 (1-2) ◽  
pp. 57-65 ◽  
Author(s):  
Fabrizio Palitti ◽  
Ruggero Ricordy ◽  
Paolo Perticone ◽  
Silvana D'andrea ◽  
Marco Rizzoni
Keyword(s):  
Actinomycin D ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

Effects of X-Irradiation on DNA Precursor Metabolism and Deoxyribonucleoside Triphosphate Pools in Chinese Hamster Cells

1974 ◽  
Vol 60 (1) ◽  
pp. 173 ◽  
Author(s):  
R. A. Walters ◽  
L. R. Gurley ◽  
R. A. Tobey ◽  
M. D. Enger ◽  
R. L. Ratliff
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
X Irradiation ◽  
Deoxyribonucleoside Triphosphate

scholarly journals TEMPORAL ORDER IN MAMMALIAN CELLS

1969 ◽  
Vol 43 (2) ◽  
pp. 207-219 ◽  
Author(s):  
Robert R. Klevecz
Keyword(s):  
Enzyme Activity ◽  
Mammalian Cells ◽  
Temporal Order ◽  
Actinomycin D ◽  
Chinese Hamster ◽  
Enzyme Synthesis ◽  
S Phase ◽  
Selection System ◽  
Enzyme Protein ◽  
Chinese Hamster Cells

Chinese hamster cells were synchronized by the Colcemid-selection system. In cells with a division cycle time of 11.5–12 hr, the activity of the enzyme lactate dehydrogenase (LDH) underwent marked oscillations with a 3.5-hr period. Precipitation of labeled LDH enzyme with specific antibody indicated that the enzyme activity changes were the result of intermittent enzyme synthesis and relatively constant degradation. Inhibition of normal DNA replication with 4 mM of thymidine, while reducing the amount of new enzyme synthesized, did not prevent oscillations from occurring. Similarly, actinomycin D (AcD) added at the time of synchronization allowed some new enzyme synthesis to proceed in an oscillatory manner. LDH synthesis went on at nearly normal rates when AcD was added in the middle of S phase. However, addition of cycloheximide to cultures at any time in the cycle caused an immediate drop in levels of activity and in enzyme protein. The half-life of LDH, calculated either from loss of enzyme activity or precipitable radioactivity in cycloheximide-treated cultures, was between 2 and 2.5 hr.

The response of histone fractions to x irradiation in cultured Chinese hamster cells

1970 ◽  
Vol 38 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Lawrence R. Gurley ◽  
Julia M. Hardin ◽  
Ronald A. Walters
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
X Irradiation
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