Reduction in Mitotic Index in Pisum sativum Root Meristems by Pulsed Ultrasound Irradiation

1976 ◽  
Vol 65 (3) ◽  
pp. 458 ◽  
Author(s):  
Kym B. Arcuri ◽  
Robert J. Pizzutiello ◽  
Morton W. Miller ◽  
Gary E. Kaufman ◽  
Edwin L. Carstensen
Keyword(s):  
Pisum Sativum ◽  
Mitotic Index ◽  
Ultrasound Irradiation ◽  
Pulsed Ultrasound ◽  
Root Meristems

scholarly journals EFECTOS DEL SORBATO DE POTASIO A DIFERENTES CONCENTRACIONES y TIEmPO DE ExPOSICIóN SOBRE EL CICLO CELULAR y EL mATERIAL GENÉTICO EN mERISTEmOS RADICULARES DE AllIuM CEPA l. (CEBOLLA)

2019 ◽  
pp. 71-78
Keyword(s):  
Cell Cycle ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Genetic Material ◽  
Potassium Sorbate ◽  
Control Group ◽  
Root Meristems ◽  
Binucleated Cells ◽  
Allium Cepa L ◽  
Ciclo Celular

EFECTOS DEL SORBATO DE POTASIO A DIFERENTES CONCENTRACIONES y TIEmPO DE ExPOSICIóN SOBRE EL CICLO CELULAR y EL mATERIAL GENÉTICO EN mERISTEmOS RADICULARES DE AllIuM CEPA l. (CEBOLLA) EFFECT OF POTASSIUm SORBATE AT DIFFERENT CONCENTRATIONS AND ExPOSURE TImE ON THE CELL CyCLE AND THE GENETIC mATERIAL IN ROOT mERISTEmS OF AllIuM CEPA l “ONION” Joselyne Quispe, José Saldaña, Tony Verde y Shirley Valderrama Universidad Nacional de Trujillo, Trujillo,Perú DOI: https://doi.org/10.33017/RevECIPeru2010.0011/ RESUMEN Con el propósito de determinar el efecto del Sorbato de Potasio sobre el ciclo celular en meristemos radiculares de Allium cepa L. “cebolla” se establecieron tres grupos experimentales que se expusieron a las soluciones de Sorbato de Potasio en concentraciones de 0.5, 1 y 2 g/L; por 6, 7 y 8 horas cada una y un grupo control. Luego se realizó la respectiva coloración en Orceína acética al 2% de acuerdo a la técnica de Tjio y Levan, observándose con el microscopio compuesto un total de 1500 células meristemáticas en cada uno de los tratamientos. Los resultados aplicando tratamiento después de 8 horas de exposición a la concentración de 2 g/L mostraron una disminución del índice mitótico de 11.26 %, mientras que se incrementó el índice profásico en 95.57 %. Se produjeron también alteraciones como rupturas cromosómicas, puentes anafásicos y células binucleadas. Las pruebas y análisis de varianza, mostraron diferencias significativas entre los diferentes tratamientos, confirmado mediante la prueba de comparación múltiple de promedios de Duncan. Se evidencia un probable efecto genotóxico en el material genético por el Sorbato de Potasio y una alteración en el ciclo celular de los meristemos de A. cepa L.; así mismo, se comprueba la función de biosensor de A. cepa L. en el estudio de sustancias que puedan afectar el ciclo celular. Palabras clave: Allium cepa, Sorbato de Potasio, Índice Mitótico, Alteración del material genético, Puentes anafásicos, Rupturas cromosómicas, Células binucleadas. ABSTRACT In order to determine the effect of Potassium Sorbate on the cell cycle in root meristems of Allium cepa L. “onion” set three experimental groups were exposed to solutions of Potassium Sorbate at concentrations of 0.5, 1 and 2g /l, for 6, 7 and 8 hours each and a control group. Later the respective color in 2% Acetic orcein according to the technique of Tjio and Levan, observed with the compound microscope a total of 1500 meristematic cells in each of the treatments. The appropriate treatment results after 8 hours of exposure to the concentration of 2 g / L showed a decrease in mitotic index of 11.26%, while it increased the rate at 95.57% prophasic index. There were also alterations as chromosome breaks, anaphasic bridges and binucleated cells. The tests and analysis of variance showed significant differences between the different treatments, as confirmed by multiple comparison test of Duncan averages. It shows a probable genotoxic effect in the genetic material by Potassium Sorbate and an alteration in the cell cycle of meristems of A. cepa L., likewise, is found biosensor function of A. cepa L. in the study of substances that may affect the cell cycle. Keywords: Allium cepa, Potassium Sorbate, Mitotic index, Abnormal genetic material, Chromosomal breaks, Anafasic bridges, Binucleated cells.

Protein-kinases and p34cdc2-like protein in root meristems of Pisum sativum during germination

Plant Science ◽  
1993 ◽  
Vol 89 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Donato Chiatante ◽  
Mariacristina Crosti ◽  
Paola Brusa ◽  
Anthony J. Trewavas ◽  
Pete C.L. John
Keyword(s):  
Pisum Sativum ◽  
Protein Kinases ◽  
Root Meristems

The effect of cell cycle regulators on protein profiles in cultured root meristems of Pisum sativum

1989 ◽  
Vol 29 (3) ◽  
pp. 317-322 ◽  
Author(s):  
William A. Tramontano ◽  
Christine A. Carman ◽  
Mary J. Sarrantonio ◽  
Anna Maria Massaro
Keyword(s):  
Cell Cycle ◽  
Pisum Sativum ◽  
Cell Cycle Regulators ◽  
Protein Profiles ◽  
Root Meristems

Nuclear proteins and the onset of cell proliferation in root meristems of Pisum sativum: QP47 a novel acidic protein

1993 ◽  
Vol 3 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Donato Chiatante ◽  
Elisabetta Onelli
Keyword(s):  
Cell Proliferation ◽  
Pisum Sativum ◽  
Polyclonal Antibody ◽  
Structural Organization ◽  
Nuclear Proteins ◽  
Proliferating Cells ◽  
Quiescent Period ◽  
Root Meristems ◽  
Embryo Cells ◽  
The Relationship

AbstractA protein named QP47 has been purified from quiescent nuclei of root meristems of Pisum sativum, and used to prepare a polyclonal antibody. Immunolocalization of this protein with fluorescent probes revealed a nuclear distribution of thread-like structures. However, the relationship between the distribution of QP47 immunofluorescence and the structural organization of the chromatin required further investigation. The decrease in content of this protein in the nuclei of embryo cells seems to be correlated with the transition from quiescence to proliferation. QP47 degradation seems to depend upon an increase in the state of its phosphorylation. This protein is not present in normally proliferating cells, or in cells whose cell proliferation has been arrested by starvation or differentiation. It is hypothesized that QP47 may be required specifically during the quiescent period for specific structural organization of the chromatin.

Pulse Frequency-dependent Regulation of Lysozyme Reactivity under Pulsed Ultrasound Irradiation

2009 ◽  
Vol 38 (6) ◽  
pp. 536-537 ◽  
Author(s):  
Takayoshi Kawasaki ◽  
Momoko Toyoda ◽  
Yoshio Okahata
Keyword(s):  
Ultrasound Irradiation ◽  
Pulse Frequency ◽  
Pulsed Ultrasound ◽  
Frequency Dependent

scholarly journals Cytological changes induced by clethodim in Pisum sativum plant

2020 ◽  
Vol 49 (2) ◽  
pp. 367-374
Author(s):  
Sazada Siddiqui ◽  
Sulaiman Alrumman
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Mitotic Index ◽  
Cytotoxic Effect ◽  
Chromosomal Anomalies ◽  
Dependent Manner ◽  
Cytological Changes ◽  
Dose Dependent ◽  
Radicle Length ◽  
Mitotic Cells

Effects of clethodim treatment on mitotic cells of P. sativum were studied in the present investigation. Pisum sativum seeds are treated with different concentrations of clethodim varying from 0.01, 0.02, 0.03 and 0.04% for 1 and 2 hrs and their effect on seed germination, radicle length, mitotic index and chromosomal anomalies were investigated. The outcomes reveal that treatment of clethodim on P. sativum seeds diminishes seed germination, radicle length, and mitotic index in dose-dependent manner. Also, in clethodim treated seeds which are time and concentration dependent, an escalation in the proportion of abnormal mitotic phases was observed. C-mitosis, fragments, precocious separation, stickiness and bridges were the most frequently observed deviations. The results revealed that frequently used herbicide clethodim has a substantial cytotoxic effect on P. sativum.

The Effect of 2 MHz Ultrasound Irradiation on Pisum sativum Roots

1976 ◽  
Vol 65 (3) ◽  
pp. 451 ◽  
Author(s):  
Morton W. Miller ◽  
Susan M. Voorhees ◽  
Edwin L. Carstensen ◽  
Gary E. Kaufman
Keyword(s):  
Pisum Sativum ◽  
Ultrasound Irradiation
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