Estimation of Life Times and Diffusion Distances of Radicals Involved in X-Ray-Induced DNA Strand Breaks or Killing of Mammalian Cells

1975 ◽  
Vol 64 (2) ◽  
pp. 306 ◽  
Author(s):  
Ruth Roots ◽  
Shigefumi Okada
Keyword(s):  
Mammalian Cells ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
X Ray ◽  
Dna Strand ◽  
And Diffusion

DNA Polymerase Required for Rapid Repair of X-ray--Induced DNA Strand Breaks in vivo

Science ◽  
1971 ◽  
Vol 172 (3985) ◽  
pp. 851-854 ◽  
Author(s):  
C. D. Town ◽  
K. C. Smith ◽  
H. S. Kaplan
Keyword(s):  
Dna Polymerase ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
X Ray ◽  
Rapid Repair ◽  
Dna Strand

Topoisomerase II inhibition prevents anaphase chromatid segregation in mammalian cells independently of the generation of DNA strand breaks

1993 ◽  
Vol 105 (2) ◽  
pp. 563-569 ◽  
Author(s):  
D.J. Clarke ◽  
R.T. Johnson ◽  
C.S. Downes
Keyword(s):  
Chromosome Segregation ◽  
Mammalian Cells ◽  
Topoisomerase Ii ◽  
Dna Strand Breaks ◽  
Temperature Sensitive ◽  
Dna Topoisomerase ◽  
Strand Breaks ◽  
Temperature Sensitive Mutants ◽  
Chromatid Segregation ◽  
Dna Strand

Yeast temperature-sensitive mutants of DNA topoisomerase II are incapable of chromosome condensation and anaphase chromatid segregation. In mammalian cells, topoisomerase II inhibitors such as etoposide (VP-16-123) have similar effects. Unfortunately, conclusions drawn from work with mammalian cells have been limited by the fact that the standard inhibitors of topoisomerase II also generate DNA strand breaks, which when produced by other agents (e.g. ionizing radiation) are known to affect progression into and through mitosis. Here we show that the anti-tumour agent ICRF-193, recently identified as a topoisomerase II inhibitor operating by a non-standard mechanism, generates neither covalent complexes between topoisomerase II and DNA, nor adjacent DNA strand breaks, in mitotic HeLa. However, the drug does prevent anaphase segregation in HeLa and PtK2 cells, with effects similar to those of etoposide. We therefore conclude that topoisomerase II function is required for anaphase chromosome segregation in mammalian cells, as it is in yeast.

Method for detecting DNA strand breaks in mammalian cells using the Deinococcus radiodurans PprA protein

2006 ◽  
Vol 596 (1-2) ◽  
pp. 36-42 ◽  
Author(s):  
Katsuya Satoh ◽  
Seiichi Wada ◽  
Masahiro Kikuchi ◽  
Tomoo Funayama ◽  
Issay Narumi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Deinococcus Radiodurans ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand

Normal Rejoining of DNA Strand Breaks in Ataxia Telangiectasia Fibroblast Lines after Low X-Ray Exposure

1981 ◽  
Vol 86 (3) ◽  
pp. 589 ◽  
Author(s):  
P. V. Hariharan ◽  
S. Eleczko ◽  
B. P. Smith ◽  
M. C. Paterson
Keyword(s):  
Ataxia Telangiectasia ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
X Ray ◽  
Dna Strand ◽  
Low X
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