The Effects of Ionizing Radiation on the Kinetics of DNA Replication in Synchronized Chinese Hamster Ovary Cells

1974 ◽  
Vol 60 (1) ◽  
pp. 62 ◽  
Author(s):  
Eugene W. Gerner ◽  
Raymond E. Meyn ◽  
Ronald M. Humphrey
Keyword(s):  
Dna Replication ◽  
Ionizing Radiation ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Kinetics Of

scholarly journals Polarity of microtubules nucleated by centrosomes and chromosomes of Chinese hamster ovary cells in vitro

1980 ◽  
Vol 84 (1) ◽  
pp. 151-159 ◽  
Author(s):  
LG Bergen ◽  
R Kuriyama ◽  
GG Borisy
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Internal Controls ◽  
Structural Explanation ◽  
Ovary Cells ◽  
Spindle Microtubules ◽  
Kinetics Of

The structural and growth polarities of centrosomal and chromosomal microtubules were studied by analyzing the kinetics of growth of these microtubules and those initiated by flagellar seeds. By comparing rates of elongation of centrosomal and flagellar-seeded microtubules, we determined whether the centrosomal microtubules were free to grow at their plus ends only, minus ends ony, or at both ends. Our results show that centrosomal microtubules elongate at a rate corresponding to the addition of subunits at the plus end only. The depolymerization rate was also equivalent to that for the plus end only. Chromosomal microtubule elongation was similar to the centrosome-initiated growth. Since the data do not support the hypothesis that both ends of these spindle microtubules are able to interact with monomer in solution, then growth must occur only distal or only proximal to the organizing centers, implying tha the opposite ends in unavailable for exchange of subunits. Experiments with flagellar-seeded microtubules serving as internal controls indicated that the inactivity of the minus end could not be accounted for by a diffusible inhibitor, suggesting a structural explanation. Since there is no apparent way in which the distal ends may be capped, whereas the proximal ends are embedded in the pericentriolar cloud, we conclude that centrosomal microtubules are oriented with their plus ends distal to the site of nucleation. A similar analysis for chromosomal microtubules suggests that they too must be oriented with their plus ends distal to the site of initiation.

scholarly journals The Kinetics of Polyethylenimine-Mediated Transfection in Suspension Cultures of Chinese Hamster Ovary Cells

2008 ◽  
Vol 40 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Martin Bertschinger ◽  
Arnaud Schertenleib ◽  
Jean Cevey ◽  
David L. Hacker ◽  
Florian M. Wurm
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Suspension Cultures ◽  
Ovary Cells ◽  
Kinetics Of
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