Repair Mechanisms and Cell Cycle Dependent Variations in X-Ray Sensitivity of Diploid Yeast

1973 ◽  
Vol 53 (2) ◽  
pp. 226 ◽  
Author(s):  
Elia Nunes de Langguth ◽  
Carl A. Beam
Keyword(s):  
Cell Cycle ◽  
X Ray ◽  
Repair Mechanisms ◽  
Cycle Dependent

Cell sorting analysis of cell cycle-dependent X-ray sensitivity in end joining-deficient human cells

2008 ◽  
Vol 372 (4) ◽  
pp. 662-667 ◽  
Author(s):  
Kuniyoshi Iwabuchi ◽  
Mitsumasa Hashimoto ◽  
Tadashi Matsui ◽  
Aya Kurosawa ◽  
Noritaka Adachi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Sorting ◽  
Human Cells ◽  
End Joining ◽  
X Ray ◽  
Cycle Dependent

Cell Cycle Dependent X-Ray-Induced Death in Chlamydomonas reinhardi

1976 ◽  
Vol 66 (2) ◽  
pp. 288 ◽  
Author(s):  
Helen E. Gruber ◽  
D. S. Nachtwey
Keyword(s):  
Cell Cycle ◽  
X Ray ◽  
Cycle Dependent

scholarly journals Activation of efficient DNA repair mechanisms after photon and proton irradiation of human chondrosarcoma cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Birgit Lohberger ◽  
Dietmar Glänzer ◽  
Nicole Eck ◽  
Sylvia Kerschbaum-Gruber ◽  
Elisabeth Mara ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Repair ◽  
Molecular Mechanisms ◽  
Proton Irradiation ◽  
Particle Therapy ◽  
Dna Damages ◽  
X Ray ◽  
Dna Repair Mechanisms ◽  
Key Players ◽  
Repair Mechanisms

AbstractAlthough particle therapy with protons has proven to be beneficial in the treatment of chondrosarcoma compared to photon-based (X-ray) radiation therapy, the cellular and molecular mechanisms have not yet been sufficiently investigated. Cell viability and colony forming ability were analyzed after X-ray and proton irradiation (IR). Cell cycle was analyzed using flow cytometry and corresponding regulator genes and key players of the DNA repair mechanisms were measured using next generation sequencing, protein expression and immunofluorescence staining. Changes in metabolic phenotypes were determined with nuclear magnetic resonance spectroscopy. Both X-ray and proton IR resulted in reduced cell survival and a G2/M phase arrest of the cell cycle. Especially 1 h after IR, a significant dose-dependent increase of phosphorylated γH2AX foci was observed. This was accompanied with a reprogramming in cellular metabolism. Interestingly, within 24 h the majority of clearly visible DNA damages were repaired and the metabolic phenotype restored. Involved DNA repair mechanisms are, besides the homology directed repair (HDR) and the non-homologous end-joining (NHEJ), especially the mismatch mediated repair (MMR) pathway with the key players EXO1, MSH3, and PCNA. Chondrosarcoma cells regenerates the majority of DNA damages within 24 h. These molecular mechanisms represent an important basis for an improved therapy.

Cell cycle dependent modulation of insulin and IGF I receptor binding to human Burkitt type ALL cells

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S81
Author(s):  
U. VETTER ◽  
W. HARTMANN ◽  
H. HITZLER ◽  
W. HEIT ◽  
J. SCHLICKENRIEDER ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Receptor Binding ◽  
Igf I ◽  
Cycle Dependent
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