The Influence of 5-Bromodeoxyuridine on the Induction of Breaks in the Deoxyribonucleic Acid of Cultivated Human Cells by X-Irradiation and Ultraviolet Light

1972 ◽  
Vol 52 (3) ◽  
pp. 627 ◽  
Author(s):  
P. H. M. Lohman ◽  
D. Bootsma ◽  
A. H. Hey
Keyword(s):  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Human Cells ◽  
X Irradiation

Effect of Repeated X-irradiation on the Process of Degradation of Deoxyribonucleic Acid

Nature ◽  
1964 ◽  
Vol 202 (4927) ◽  
pp. 106-107 ◽  
Author(s):  
B. MILETIĆ ◽  
Ž. KUĆAN ◽  
DJ. NOVAK
Keyword(s):  
Deoxyribonucleic Acid ◽  
X Irradiation

Cockayne Syndrome: A Cellular Sensitivity to Ultraviolet Light

PEDIATRICS ◽  
1977 ◽  
Vol 60 (2) ◽  
pp. 135-139
Author(s):  
R. D. Schmickel ◽  
E. H. Y. Chu ◽  
J. E. Trosko ◽  
C. C. Chang
Keyword(s):  
Ultraviolet Light ◽  
Uv Light ◽  
Cockayne Syndrome ◽  
Fibroblast Cultures ◽  
Cellular Sensitivity ◽  
X Irradiation ◽  
Increased Sensitivity ◽  
Cellular Dna ◽  
Thymidine Dimer ◽  
Enzymatic Defect

Two unrelated children, a boy 2½ years old and a girl 4 years old, were affected with the cachectic dwarfism of Cockayne syndrome. Fibroblast cultures derived from these patients exhibited increased sensitivity to ultraviolet (UV) light, but not to x-irradiation, as measured by colony-forming ability. In both Cockayne fibroblast cultures, the rate of removal of thymidine dimer from the irradiated cellular DNA was normal. This demonstration of a cellular defect in Cockayne cells suggests that there may be an enzymatic defect in the repair of UV light-induced damage.

Analysis of point mutations induced by ultraviolet light in human cells

1991 ◽  
Vol 249 (1) ◽  
pp. 147-159 ◽  
Author(s):  
Phouthone Keohavong ◽  
Vivian F. Liu ◽  
William G. Thilly
Keyword(s):  
Ultraviolet Light ◽  
Point Mutations ◽  
Human Cells

COMPARISON OF THE EFFECTS OF VISIBLE, ULTRAVIOLET, AND X-RADIATION ON RAT THYMOCYTES

1964 ◽  
Vol 42 (4) ◽  
pp. 529-543 ◽  
Author(s):  
D. K. Myers ◽  
Donna DeWolfe-Slade
Keyword(s):  
Nucleic Acid ◽  
Visible Light ◽  
Ultraviolet Light ◽  
Intact Cell ◽  
Lethal Effect ◽  
Low Doses ◽  
X Irradiation ◽  
Thymus Cells ◽  
Ultraviolet Energy

Ultraviolet and X-irradiation produce many of the same effects on rat thymocytes in vitro. Exposure of the cells to low doses results in a latent lethal effect, which requires incubation at 37 °C for its expression, and in an apparent increase in the rate at which deoxyribonucleoprotein from the cells dissolves to form a gel in 2 M NaCl. As the doses are increased, a decrease in the viscosity of the deoxyribonucleoprotein gel, an immediate "death" and swelling of the cells, and an immediate destruction of nucleotide bases become evident. For either radiation, doses which do not appear to have any immediate effect on the intact cell lead to loss of soluble materials (potassium ion, ribonucleotides) from the cells after incubation at 37 °C for several hours. The amount of ultraviolet energy required to produce most of the above effects is nearly 1000 times greater than the amount of X-ray energy required to produce the same effect.Three differences between the effects of ultraviolet and X-irradiation were observed: Ultraviolet light was relatively inefficient in breaking down the long deoxyribonucleoprotein chains but appeared to destroy hydrogen-bonding in the native nucleic acid structure at the same time as it destroyed the nucleotide basis. Moreover, the lethal effects of low doses of ultraviolet light on thymus cells require a longer time to develop than do the effects of X-irradiation.Thymocytes can also be killed by high intensities of visible light, particularly in the presence of photoreducible dyes. However, visible light produces little or no latent damage to the cells, nor was any evidence of nucleic acid damage observed.

scholarly journals The lacI shuttle: rapid analysis of the mutagenic specificity of ultraviolet light in human cells.

1985 ◽  
Vol 82 (24) ◽  
pp. 8606-8610 ◽  
Author(s):  
J. S. Lebkowski ◽  
S. Clancy ◽  
J. H. Miller ◽  
M. P. Calos
Keyword(s):  
Ultraviolet Light ◽  
Rapid Analysis ◽  
Human Cells

Lethal and mutagenic action of 3200–4000 Å light

1968 ◽  
Vol 14 (6) ◽  
pp. 727-735 ◽  
Author(s):  
S. J. Webb ◽  
C. C. Tai
Keyword(s):  
Escherichia Coli ◽  
Relative Humidity ◽  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Auxotrophic Mutant ◽  
Mutagenic Action ◽  
Black Light ◽  
Hydrolysis Of ◽  
Escherichia Coli B

Cells of a thymine-requiring auxotrophic mutant of Escherichia coli B have been irradiated with 2537 Å light (ultraviolet) and 3200–4000 Å light (black light) while being held in aerosols of various relative humidity (R.H.) levels. When cells were held in aerosols of 70% R.H. or lower they became susceptible to damage by black light and much of this damage could be prevented by the compound myo-inositol. The damage inflicted on cells by black light was not photorepairable by the usual methods, suggesting that the lesions produced are different from those produced by ultraviolet light. In addition, the ability of cells to undergo photorepair after irradiation with 2537 Å light was found to decrease rapidly when the cells were irradiated in a dry or near-dry state, indicating that the lesions produced under these conditions are different from those produced in wet cells.Sensitization of the cells to both kinds of radiations by the presence of bromodeoxyuridine (BUDR) in their deoxyribonucleic acid was apparent only when the cells were irradiated in a wet or semidry state, suggesting that sensitization involves a photostimulated hydrolysis of BUDR. Black light was found to be more mutagenic to cells held in a semidried state than was 2537 Å light. It is concluded that the irradiation of cells with 2537 Å light or with black light when they are in the dry state produces a lesion which is non-photorepairable and which is both lethal and mutagenic.

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