The Response of Rat Liver Enzymes Involved in Fatty Acid Synthesis to Increasing Doses of Ionizing Radiation in Vivo

1972 ◽  
Vol 52 (3) ◽  
pp. 545 ◽  
Author(s):  
C. G. McHale ◽  
G. N. Catravas
Keyword(s):  
Fatty Acid ◽  
Ionizing Radiation ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Liver Enzymes ◽  
Acid Synthesis

scholarly journals Regulation of carnitine palmitoyltransferase activity by malonyl-CoA in mitochondria from sheep liver, a tissue with a low capacity for fatty acid synthesis

1985 ◽  
Vol 232 (1) ◽  
pp. 177-182 ◽  
Author(s):  
N P Brindle ◽  
V A Zammit ◽  
C I Pogson
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Liver Mitochondria ◽  
Sheep Liver ◽  
Malonyl Coa ◽  
Cpt I ◽  
Guinea Pig Liver

The characteristics of inhibition of carnitine palmitoyltransferase (CPT) I by malonyl-CoA were studied for the enzyme in mitochondria isolated from sheep liver, a tissue with a very low rate of fatty acid synthesis. Malonyl-CoA was as potent in inhibiting the sheep liver enzyme as in inhibiting the enzyme in rat liver mitochondria. CPT I in guinea-pig liver mitochondria was also similarly inhibited. The inhibition showed the same time-dependent characteristics previously established for the rat liver enzyme. Methylmalonyl-CoA was as effective an inhibitor of CPT I as malonyl-CoA in sheep liver mitochondria, but did not affect CPT I activity in mitochondria from rat or guinea-pig liver. The concentrations of malonyl-CoA required to inhibit CPT I in sheep liver mitochondria in vitro were similar to those found in freeze-clamped sheep liver samples (about 7 nmol of malonyl-CoA/g wet wt.). In sheep liver cells the content of malonyl-CoA was only one-tenth of that observed in vivo when glucose only was added to the incubation medium. Inclusion of acetate and/or insulin increased the malonyl-CoA content about 10-fold, to values similar to those observed in vivo. The rate of fatty acid synthesis in sheep liver cells was about 1% of that observed in rat liver, but was correlated with the concentrations of malonyl-CoA in the cells under various incubation conditions. These observations are discussed in relation to (i) the regulatory role of malonyl-CoA in tissues that have a low capacity for fatty acid synthesis, and (ii) the utilization by sheep liver of propionate as a gluconeogenic precursor.

scholarly journals THE ESTIMATION OF FATTY ACID SYNTHESIS IN RAT LIVER SLICES

1953 ◽  
Vol 205 (1) ◽  
pp. 401-408
Author(s):  
Grace Medes ◽  
Morris A. Spirtes ◽  
Sidney Weinhouse
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Liver Slices

scholarly journals Influence of Kynurenate on Cholesterol and Fatty Acid Synthesis in Isolated Perfused Rat Liver

1973 ◽  
Vol 248 (2) ◽  
pp. 738-739
Author(s):  
Christian A. Barth ◽  
H. Jürgen Hackenschmidt ◽  
Elmar E. Weis ◽  
Karl F.A. Decker
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

EFFECT OF ALLOXAN, INSULIN, AND THYROXINE ON CHOLESTEROL AND FATTY ACID SYNTHESIS BY RAT LIVER HOMOGENATES

1957 ◽  
Vol 35 (1) ◽  
pp. 15-23 ◽  
Author(s):  
J. F. Scaife ◽  
B. B. Migicovsky
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Sodium Acetate ◽  
Cholesterol Synthesis ◽  
Acid Synthesis ◽  
Liver Homogenates ◽  
Vitro Effect

The in vitro effect of alloxan and insulin on the synthesis of cholesterol and fatty acids from 1-C14-sodium acetate by rat liver homogenates has been examined. Alloxan caused a reduction in the incorporation of acetate into cholesterol, fatty acids, and C14O2, but an increase in the oxygen consumption and carbon dioxide production. The addition of insulin to homogenates caused a reduction in cholesterol synthesis but an increase in fatty acid synthesis both for normal and diabetic animals. Homogenates from thyrotoxic rats exhibited a marked reduction in cholesterol synthesis when compared with normal animals. C14O2 production by homogenates from starved rats was appreciably lower than for those from normal animals. With this exception no appreciable difference was found in the oxygen uptake, carbon dioxide, or C14O2 production in homogenates from normal, starved, thyroxine-treated, or diabetic animals. Synthesized cholesterol was found to be located principally in the particulate matter of the homogenates after they had been incubated with 1-C14-sodium acetate. Homogenates from starved rats showed no greater tendency to degrade preformed cholesterol during incubation than did those from normal rats.

Lipogenesis in the Ruminant: in vivo Site of Fatty Acid Synthesis in Sheep

1972 ◽  
Vol 102 (5) ◽  
pp. 617-623 ◽  
Author(s):  
D. L. Ingle ◽  
D. E. Bauman ◽  
U. S. Garrigus
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis
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