The Relation of Protein Synthesis to Radiation-Induced Division Delay in Chinese Hamster Cells

1970 ◽  
Vol 44 (3) ◽  
pp. 788 ◽  
Author(s):  
Silvia Bacchetti ◽  
Warren K. Sinclair
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Radiation Induced

Protein synthesis and the presence or absence of a measurable G1 in cultured chinese hamster cells

1980 ◽  
Vol 104 (3) ◽  
pp. 461-467 ◽  
Author(s):  
R. Michael Liskay ◽  
Bruce Kornfeld ◽  
Paul Fullerton ◽  
Ronald Evans
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

scholarly journals REPAIR OF RADIATION-INDUCED LETHAL AND MUTATIONAL DAMAGE IN CHINESE HAMSTER CELLS IN VITRO

1979 ◽  
Vol 54 (2) ◽  
pp. 109-119 ◽  
Author(s):  
FUMIO SUZUKI ◽  
HIROYOSHI HOSHI ◽  
MASAKATSU HORIKAWA
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Radiation Induced ◽  
Mutational Damage

scholarly journals Chinese hamster cells can be reversibly blocked before mitosis with the protein synthesis inhibitor, emetine

1983 ◽  
Vol 59 (1) ◽  
pp. 257-268
Author(s):  
J.T. Westwood ◽  
E.B. Wagenaar
Keyword(s):  
Protein Synthesis ◽  
Mitotic Index ◽  
Chinese Hamster ◽  
Inhibitory Effect ◽  
Eukaryotic Cells ◽  
Protein Synthesis Inhibitor ◽  
Synthesis Inhibitor ◽  
Chinese Hamster Cells ◽  
Initial Concentration ◽  
G2 Phase

The inhibition of protein synthesis in eukaryotic cells will prevent them from entering mitosis. Emetine inhibits peptide elongation. When it was added to asynchronous populations of Chinese hamster ovary (CHO) cells, the mitotic index decreased sharply 30 to 40 min later. It was found that the inhibitory effect of emetine could be reversed when it was removed and the reversibility was dependent on both the initial concentration of emetine and the pH of the medium. Cell populations that were blocked by emetine for up to 2h showed a four- to fivefold increase in mitotic index approximately 1 h after the emetine was removed. These results indicate that there is a point or period in G2 phase at which critical ‘mitotic proteins’ are being synthesized, and if their synthesis is interrupted cells will fail to enter mitosis.

Radiation-Induced Cycle Delay in Synchronized Chinese Hamster Cells: Comparison between DNA Synthesis and Division

1973 ◽  
Vol 53 (2) ◽  
pp. 326 ◽  
Author(s):  
Dennis B. Leeper ◽  
Martin H. Schneiderman ◽  
William C. Dewey
Keyword(s):  
Dna Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Radiation Induced
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