Repopulation of Bone Marrow in Mice: Number and Type of Cells Required for Post-X-Irradiation Protection

1965 ◽  
Vol 25 (1) ◽  
pp. 53 ◽  
Author(s):  
N. B. Kurnick ◽  
Naime Nokay
Keyword(s):  
Bone Marrow ◽  
X Irradiation ◽  
Irradiation Protection

Effects of Low-Level (1.0 R) X-Irradiation on the Erythroid Response of the Rat Bone Marrow

1976 ◽  
Vol 65 (1) ◽  
pp. 83 ◽  
Author(s):  
J. K. Gong ◽  
C. A. Glomski ◽  
N. L. Frederiksen ◽  
A. J. Lawson ◽  
J. P. Daley
Keyword(s):  
Bone Marrow ◽  
Low Level ◽  
Erythroid Response ◽  
X Irradiation ◽  
Rat Bone

X-Irradiation and Bone Marrow

1957 ◽  
Vol 65 (4) ◽  
pp. 599-606
Author(s):  
A. de Schryver ◽  
G. Demeester ◽  
I. Leusen
Keyword(s):  
Bone Marrow ◽  
X Irradiation

scholarly journals Effect of Chemical Protection and Bone Marrow Treatment on Radiation Injury in Mice

Blood ◽  
1958 ◽  
Vol 13 (7) ◽  
pp. 665-676 ◽  
Author(s):  
PAUL URSO ◽  
C. C. CONGDON ◽  
D. G. DOHERTY ◽  
RAYMOND SHAPIRA
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Radiation Injury ◽  
Combined Treatment ◽  
X Rays ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Hematopoietic Organs ◽  
X Irradiation ◽  
Bone Marrow Granulocytes

Abstract MEG (prepared from 9.0 mg. of AET) significantly modified the response of the bone marrow, peripheral blood leukocytes, spleen, thymus, body weight, hematocrit, and histology of the hematopoietic organs to lethal (900 r) and sublethal (450 r) x-irradiation in CAF1 mice. MEG reduced the effect of 900 r on the bone marrow, granulocytes of the blood, hematocrit, spleen, thymus, and body weight by a factor of approximately two. Combined treatment (MEG and isologous bone marrow) of mice exposed to 900 r of x-rays demonstrated that MEG is primarily responsible for preventing the early destruction of the bone marrow, but bone marrow injection was primarily responsible for causing a more rapid recovery of the bone marrow. In mice receiving combined treatment, recovery of the leukocytes and spleen was primarily influenced by the bone marrow injection; whereas recovery of the thymus and body weight was primarily influenced by MEG. The hematocrit values were normal after combined treatment.

Bone-marrow Mitotic Inhibition induced by Local Splenic X-irradiation

Nature ◽  
1963 ◽  
Vol 200 (4912) ◽  
pp. 1221-1222 ◽  
Author(s):  
P. A. MAURICE ◽  
A. JEANRENAUD
Keyword(s):  
Bone Marrow ◽  
Mitotic Inhibition ◽  
X Irradiation

scholarly journals Regeneration of blood-forming organs after autologous leukocyte transfusion in lethally irradiated dogs. II. Distribution and cellularity of the marrow in irradiated and transfused animals

Blood ◽  
1976 ◽  
Vol 47 (4) ◽  
pp. 593-601 ◽  
Author(s):  
W Calvo ◽  
TM Fliedner ◽  
E Herbst ◽  
E Hugl ◽  
C Bruch
Keyword(s):  
Bone Marrow ◽  
Normal Values ◽  
Whole Body ◽  
Blood Leukocytes ◽  
X Irradiation ◽  
Trabecular Bones ◽  
Marrow Cellularity ◽  
Number Of Cells

Dogs were given transfusions of cryopreserved autologous mononuclear blood leukocytes after 1200 roentgens (R) (midline dose) whole-body x- irradiation. Bone marrow repopulation was studied by means of histomorphological methods at days 9 and 10 after transfusion of an average of 3 X 10(9), 7 X 10(9), 13 X 10(9), and 31 X 10(9) cells. The return of marrow cellularity to normal values was related to the number of cells transfused. With low cell doses (3 X 10(9) and 7 X 10(9)), the marrow regeneration at 10 days was focal. There were groups of cells (colonies) showing either erythropoiesis, myelopoiesis, or megakaryocytopoiesis in the osteal niches of the trabecular bones. Frequently such niches were seen showing complete cellular recovery next to niches with complete aplasia. With higher cell doses, all niches showed hemopoietic regeneration, and the cellularity approached normal values. No hemopoietic regeneration was observed in those skeletal parts that do not show hemopoiesis, even under normal circumstances.

scholarly journals Some Factors Involved in the Effect of X-Irradiation on the Phosphatase Activity of Hematopoietic Tissues

Blood ◽  
1959 ◽  
Vol 14 (10) ◽  
pp. 1128-1136 ◽  
Author(s):  
EDWIN M. UYEKI ◽  
PAUL R. SALERNO
Keyword(s):  
Bone Marrow ◽  
Enzyme Activity ◽  
Peripheral Blood ◽  
Adenosine Triphosphatase ◽  
Lymphoid Tissues ◽  
X Ray ◽  
Adenosine Triphosphatase Activity ◽  
Assay Medium ◽  
X Irradiation ◽  
Total Body

Abstract Factors which modify lymphoid distribution of tissues were found to modify the adenosine triphosphatase activity of these tissues. Starvation or cortisone injection, which produces destructive changes in lymphoid tissues, was found to increase the enzyme activity of spleen and thymus tissues. The greater increment of enzyme activity of the thymus as compared to that of the spleen was correlated with its normally higher content of lymphoid tissue. The increase in adenosine triphosphatase activity of hematopoietic tissues appears to be associated with the type of cells present in the assay medium. With respect to peripheral blood leukocytes of the rat, the cell type is confined largely to lymphocytes and granulocytes. The increase in adenosine triphosphatase activity of the leukocytes after total-body x-ray was seen to parallel the increase in granulocytes present in the assay medium. The ratio of granulocytes to lymphocytes is not appreciably altered in dog peripheral blood after exposure to total-body x-ray; the adenosine triphosphatase activity similarly was not significantly altered. After total-body x-ray (390 r and 780 r), cells isolated from the rat bone marrow displayed a fivefold increase in adenosine triphosphatase activity. This increase was seen to correspond with an increase in the ratio of segmented leukocytes and reticuloendothelial cells and a decrease in the immature forms of the erythroid and myeloid cells. The heterogeneous cell mixtures used for our assay procedures permit the observation that total-body x-irradiation results in an increased enzyme activity of the isolated cells of the peripheral blood, bone marrow and spleen tissue of the rat. The increased enzyme activity was associated with the increased ratio of cells with high enzyme activity present in the assay medium.

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