Action of Tritiated Thymidine on the Clonal Growth of Mammalian Cells

1959 ◽  
Vol 11 (4) ◽  
pp. 535 ◽  
Author(s):  
Ruth M. Drew ◽  
Robert B. Painter
Keyword(s):  
Clonal Growth ◽  
Mammalian Cells ◽  
Tritiated Thymidine

Studies of the 'Adaptive' Repair Response in Human Lymphocytes and V79 Cells after Treatment with MNNG and MNU

1988 ◽  
Vol 7 (4) ◽  
pp. 337-341 ◽  
Author(s):  
D. Anderson ◽  
P. Fisher ◽  
P.C. Jenkinson ◽  
B.J. Phillips
Keyword(s):  
Adaptive Response ◽  
Mammalian Cells ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Alternative Methods ◽  
X Rays ◽  
V79 Cells ◽  
Repair Mechanisms ◽  
Experimental Protocols ◽  
Mutagenic Effects

In bacteria, there is evidence that a damage inducible repair response system known as the adaptive response exists since pretreatment with low doses of a simple monofunctional alkylating agent leads to a decrease in both the lethal and mutagenic effects of a subsequent challenge dose of the agent. The evidence for an analagous system in mammalian cells has proved to be inconsistent to date. The induction of chromosome repair mechanisms in human cells by low-dose radiation from tritiated thymidine has been shown to make the cells refractory to the induction of chromosome aberrations by X-rays. The present communication investigates the induction of an adaptive response in human lymphocytes from four donors and V79 cells using SCE and mutation as endpoints and MNNG and MNU for the adapting and challenging treatment. It is clear that a reproducible model of the adaptive response in human lymphocytes is difficult to establish because of the variability between different donors and different culture times. In V79 cells, assays with much larger cell numbers are required to detect a reproducible response with such small changes in mutant frequency. To demonstrate an adaptive response conclusively in mammalian cells will probably require the use of more sensitive experimental protocols and alternative methods of administration of adaptive doses of mutagen.

Synchronization of Mammalian Cells with Tritiated Thymidine

Science ◽  
1966 ◽  
Vol 151 (3711) ◽  
pp. 691-694 ◽  
Author(s):  
G. F. Whitmore ◽  
S. Gulyas
Keyword(s):  
Mammalian Cells ◽  
Tritiated Thymidine

Chapter 3 Recording of Clonal Growth of Mammalian Cells through Many Generations

1976 ◽  
pp. 15-27 ◽  
Author(s):  
Hiroshi Miyamoto ◽  
Leif Rasmussen ◽  
Erik Zeuthen
Keyword(s):  
Clonal Growth ◽  
Mammalian Cells
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