Sequence and Secondary Structure Variation in the Gyrodactylus (Platyhelminthes: Monogenea) Ribosomal RNA Gene Array

2000 ◽  
Vol 86 (3) ◽  
pp. 567 ◽  
Author(s):  
Carey O. Cunningham ◽  
Holly Aliesky ◽  
Catherine M. Collins
Keyword(s):  
Secondary Structure ◽  
Ribosomal Rna ◽  
Gene Array ◽  
Structure Variation ◽  
Ribosomal Rna Gene

Characterization of the Gyrodactylus salaris Malmberg, 1957 (Platyhelminthes: Monogenea) ribosomal intergenic spacer (IGS) DNA

Parasitology ◽  
2000 ◽  
Vol 121 (5) ◽  
pp. 555-563 ◽  
Author(s):  
C. M. COLLINS ◽  
C. O. CUNNINGHAM
Keyword(s):  
Ribosomal Rna ◽  
Tandem Repeats ◽  
Gene Array ◽  
Pcr Amplification ◽  
Intergenic Spacer ◽  
Sequence Motifs ◽  
Ribosomal Rna Gene ◽  
Intergenic Spacers ◽  
Transcription Start Sites ◽  
Pcr Products

The intergenic spacer of the ribosomal RNA gene array from the monogenean Gyrodactylus salaris was isolated using PCR amplification. PCR products were cloned and sequenced. Three different fragments of 0·63, 1·0 and 2·62 kb, were consistently obtained. These showed homology at the 5′ and 3′ termini but differed in their overall size and intervening sequence. The 5′ end showed homology to various 28S ribosomal RNA gene sequences, suggesting that this represented the 3′ terminus of the G. salaris 28S ribosomal RNA gene. A number of features common to other eukaryotic intergenic spacers were found in the longest sequence, including A + T rich sequences, palindromic sequences and tandemly repeated elements. Two regions of 23 bp sequences arranged in non-identical tandem repeats were identified. There were 9 repeats in both regions, separated by 81 bp of non-repetitive sequence. The repeat units from the two regions shared some similarity at their 3′ ends. The G. salaris intergenic spacer sequence was examined for sequence motifs involved in the transcription of the ribosomal RNA genes in other species. Several regions with homology to transcription start sites were identified.

scholarly journals Nucleotide sequence of a crustacean 18S ribosomal RNA gene and secondary structure of eukaryotic small subunit ribosoraal RNAs

1984 ◽  
Vol 12 (23) ◽  
pp. 8749-8768 ◽  
Author(s):  
Luc Nelles ◽  
Bao-Ling Fang ◽  
Guido Volckaert ◽  
Antoon Vandenberghe ◽  
Rupert De Wachter
Keyword(s):  
Secondary Structure ◽  
Nucleotide Sequence ◽  
Ribosomal Rna ◽  
Small Subunit ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene

Cloning and sequence analysis of the 18 S ribosomal RNA gene of tomato and a secondary structure model for the 18 S rRNA of angiosperms

1989 ◽  
Vol 219 (1-2) ◽  
pp. 17-25 ◽  
Author(s):  
Waltraud Schmidt-Puchta ◽  
Gertrud Kütemeier ◽  
Isolde Günther ◽  
Bernd Haas ◽  
Heinz L. Sänger
Keyword(s):  
Sequence Analysis ◽  
Secondary Structure ◽  
Ribosomal Rna ◽  
Structure Model ◽  
Secondary Structure Model ◽  
Ribosomal Rna Gene ◽  
18 S Ribosomal Rna
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