Host Cell Apoptosis Impairs Cryptosporidium parvum Development In vitro

2000 ◽  
Vol 86 (5) ◽  
pp. 922
Author(s):  
Giovanni Widmer ◽  
Elizabeth A. Corey ◽  
Barry Stein ◽  
Jeffrey K. Griffiths ◽  
Saul Tzipori
Keyword(s):  
Host Cell ◽  
Cryptosporidium Parvum ◽  
Cell Apoptosis ◽  
Host Cell Apoptosis ◽  
Development In Vitro

scholarly journals Cryptosporidium parvum at Different Developmental Stages Modulates Host Cell Apoptosis In Vitro

2004 ◽  
Vol 72 (10) ◽  
pp. 6061-6067 ◽  
Author(s):  
Raffaella Mele ◽  
Maria Angeles Gomez Morales ◽  
Fabio Tosini ◽  
Edoardo Pozio
Keyword(s):  
Cell Culture ◽  
Host Cell ◽  
Cryptosporidium Parvum ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Active Form ◽  
Annexin V ◽  
Apoptotic Cells ◽  
Host Cell Apoptosis ◽  
Infected Cells

ABSTRACT We studied apoptosis in a human ileocecal adenocarcinoma tumor cell line (HCT-8) infected with Cryptosporidium parvum, from 2 to 72 h postinfection (h.p.i.). At 2 h.p.i., the percentage of annexin V-positive cells in the cell culture had increased to 10% compared to 2.5% in noninfected control culture; sorted infected cells expressed mRNA of FasL, the active form of caspase 3, and high caspase 3 activity, whereas the noninfected neighboring cells sorted from the same culture showed no signs of apoptosis. At 24 h.p.i., the percentages of early (annexin V positive) and late (DNA fragment) apoptotic cells were 13 and 2%, respectively, in the entire cell culture, and these percentages were not statistically significant in comparison with those from noninfected control cultures. At this time, sorted infected cells expressed the inactive form of caspase 3, a low caspase 3 activity, and the antiapoptotic protein Bcl-2. Noninfected cells sorted from the same culture showed expression of the active form of caspase 3, a moderate caspase 3 activity, and no Bcl-2 expression. At 48 h.p.i., the percentages of early and late apoptotic cells and caspase 3 activity had increased in the total cell culture, and both sorted infected and noninfected cells showed the active form of caspase 3. These results show that C. parvum, depending on its developmental stage, can inhibit (at the trophozoite stage) or promote (at the sporozoite and merozoite stages) host cell apoptosis, suggesting that it is able to interact with and regulate the host-cell gene expression.

Trypanosoma cruzi: Alpha-2-macroglobulin regulates host cell apoptosis induced by the parasite infection in vitro

2008 ◽  
Vol 118 (3) ◽  
pp. 331-337 ◽  
Author(s):  
E.M. De Souza ◽  
M. Meuser-Batista ◽  
D.G. Batista ◽  
B.B. Duarte ◽  
T.C. Araújo-Jorge ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Apoptosis ◽  
Parasite Infection ◽  
Host Cell Apoptosis

Porcine IFI30 inhibits PRRSV proliferation and host cell apoptosis in vitro

Gene ◽  
2018 ◽  
Vol 649 ◽  
pp. 93-98 ◽  
Author(s):  
Jia Guo ◽  
Mengjiao Zhou ◽  
Xin Liu ◽  
Yunzhi Pan ◽  
Runjun Yang ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Apoptosis ◽  
Host Cell Apoptosis

scholarly journals P100W4-2 Analysis of host cell apoptosis in vitro and in vivo by Trypanosoma cruzi infection

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 102
Author(s):  
Junko SHIMADA ◽  
Chunbin ZOU ◽  
Takeshi NARA ◽  
Miriam PONSTAN ◽  
Takashi AOKI ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Apoptosis ◽  
Host Cell Apoptosis ◽  
Cruzi Infection

HOST CELL APOPTOSIS IMPAIRSCRYPTOSPORIDIUM PARVUMDEVELOPMENT IN VITRO

2000 ◽  
Vol 86 (5) ◽  
pp. 922-928 ◽  
Author(s):  
Giovanni Widmer ◽  
Elizabeth A. Corey ◽  
Barry Stein ◽  
Jeffrey K. Griffiths ◽  
Saul Tzipori
Keyword(s):  
Host Cell ◽  
Cell Apoptosis ◽  
Host Cell Apoptosis

Characterization of a cysteine protease from Tritrichomonas foetus that induces host-cell apoptosis

2008 ◽  
Vol 477 (2) ◽  
pp. 239-243 ◽  
Author(s):  
John J. Lucas ◽  
Gary R. Hayes ◽  
Hardip K. Kalsi ◽  
Robert O. Gilbert ◽  
Yongchool Choe ◽  
...  
Keyword(s):  
Host Cell ◽  
Cysteine Protease ◽  
Cell Apoptosis ◽  
Tritrichomonas Foetus ◽  
Host Cell Apoptosis
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