Susceptibility of Guyanan Saimiri Monkeys to a Chloroquine-Sensitive and a Chloroquine-Resistant Strain of Plasmodium vivax from Papua New Guinea

1995 ◽  
Vol 81 (4) ◽  
pp. 640 ◽  
Author(s):  
R. D. Cooper
Keyword(s):  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Resistant Strain ◽  
New Guinea

scholarly journals High-throughput molecular diagnosis of circumsporozoite variants VK210 and VK247 detects complex Plasmodium vivax infections in malaria endemic populations in Papua New Guinea

2011 ◽  
Vol 11 (2) ◽  
pp. 391-398 ◽  
Author(s):  
Cara N. Henry-Halldin ◽  
Daphne Sepe ◽  
Melinda Susapu ◽  
David T. McNamara ◽  
Moses Bockarie ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Molecular Diagnosis ◽  
High Throughput ◽  
New Guinea

scholarly journals The epidemiology of Plasmodium falciparum and Plasmodium vivax in East Sepik Province, Papua New Guinea, pre- and post-implementation of national malaria control efforts

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Johanna H. Kattenberg ◽  
Dulcie L. Gumal ◽  
Maria Ome-Kaius ◽  
Benson Kiniboro ◽  
Matthew Philip ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Malaria Control ◽  
New Guinea

scholarly journals The clinical and public health problem of relapse despite primaquine therapy: case review of repeated relapses of Plasmodium vivax acquired in Papua New Guinea

2014 ◽  
Vol 13 (1) ◽  
pp. 488 ◽  
Author(s):  
R Joan H Ingram ◽  
Chelzie Crenna-Darusallam ◽  
Saraswati Soebianto ◽  
Rintis Noviyanti ◽  
J Baird
Keyword(s):  
Public Health ◽  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Health Problem ◽  
Public Health Problem ◽  
New Guinea ◽  
Case Review

scholarly journals Infectivity of symptomatic malaria patients to Anopheles farauti colony mosquitoes in Papua New Guinea

2020 ◽  
Author(s):  
Lincoln Timinao ◽  
Rebecca Vinit ◽  
Michelle Katusele ◽  
Tamarah Koleala ◽  
Elma Nate ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Venous Blood ◽  
New Guinea ◽  
Anopheles Farauti ◽  
Symptomatic Malaria ◽  
Membrane Feeding ◽  
Transmission Studies ◽  
Mosquito Infection

AbstractDespite being a weak point in their life cycle, transmission of Plasmodium parasites from humans to mosquitoes is an understudied field of research. Direct membrane feeding assays (DMFA) are an important tool, allowing detailed mechanistic malaria transmission studies from humans to mosquitoes. Especially for Plasmodium vivax, which cannot be cultured long-term under laboratory conditions, implementation of DMFAs requires proximity to P. vivax endemic areas. In the present study, we investigated the infectivity of symptomatic Plasmodium infections to Anopheles farauti colony mosquitoes in Papua New Guinea (PNG), a country with one of the highest rates of Plasmodium vivax in the world. A total of 182 DMFAs were performed with venous blood collected from symptomatic malaria patients positive by rapid diagnostic test (RDT). DMFAs resulted in mosquito infection in 20.9% (38/182) of cases. The parasite species in the blood feeds were determined retrospectively by expert light microscopy and quantitative real-time qPCR. Based on light microscopy, 9.2% of P. falciparum and 42% of P. vivax human infections resulted in mosquito infections. Infections containing gametocytes detected by microscopy led to mosquito infections in 58.8% of P. vivax and 8.7% of P. falciparum infections. Based on qPCR, 10% of P. falciparum and 43.6% of P. vivax lead to a successful mosquito infection. Venous blood samples from symptomatic P. vivax patients were more infectious to An. farauti mosquitoes in DMFAs compared to P. falciparum infected patients. The capacity to perform DMFAs in a high-burden P. vivax setting creates a unique opportunity to address critical gaps in our understanding of P. vivax human-tomosquito transmission.

scholarly journals Novel Plasmodium vivax dhfr Alleles from the Indonesian Archipelago and Papua New Guinea: Association with Pyrimethamine Resistance Determined by a Saccharomyces cerevisiae Expression System

2005 ◽  
Vol 49 (2) ◽  
pp. 733-740 ◽  
Author(s):  
Michele D. Hastings ◽  
Jason D. Maguire ◽  
Michael J. Bangs ◽  
Peter A. Zimmerman ◽  
John C. Reeder ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Papua New Guinea ◽  
Expression System ◽  
Point Mutations ◽  
New Guinea ◽  
Indonesian Archipelago ◽  
Yeast Assay ◽  
Antifolate Resistance ◽  
Very High

ABSTRACT In plasmodia, the dihydrofolate reductase (DHFR) enzyme is the target of the pyrimethamine component of sulfadoxine-pyrimethamine (S/P). Plasmodium vivax infections are not treated intentionally with antifolates. However, outside Africa, coinfections with Plasmodium falciparum and P. vivax are common, and P. vivax infections are often exposed to S/P. Cloning of the P. vivax dhfr gene has allowed molecular comparisons of dhfr alleles from different regions. Examination of the dhfr locus from a few locations has identified a very diverse set of alleles and showed that mutant alleles of the vivax dhfr gene are prevalent in Southeast Asia where S/P has been used extensively. We have surveyed patient isolates from six locations in Indonesia and two locations in Papua New Guinea. We sequenced P. vivax dhfr alleles from 114 patient samples and identified 24 different alleles that differed from the wild type by synonymous and nonsynonymous point mutations, insertions, or deletions. Most importantly, five alleles that carried four or more nonsynonymous mutations were identified. Only one of these highly mutant alleles had been previously observed, and all carried the 57L and 117T mutations. P. vivax cannot be cultured continuously, so we used a yeast assay system to determine in vitro sensitivity to pyrimethamine for a subset of the alleles. Alleles with four nonsynonymous mutations conferred very high levels of resistance to pyrimethamine. This study expands significantly the total number of novel dhfr alleles now identified from P. vivax and provides a foundation for understanding how antifolate resistance arises and spreads in natural P. vivax populations.

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