Resumption of Feeding In vitro by Hookworm Third-Stage Larvae: A Comparative Study

1992 ◽  
Vol 78 (6) ◽  
pp. 1036 ◽  
Author(s):  
John M. Hawdon ◽  
Susan W. Volk ◽  
D. I. Pritchard ◽  
G. A. Schad
Keyword(s):  
Comparative Study ◽  
Third Stage

scholarly journals Correction: Antonelli, A., et al. Can Bone Compaction Improve Primary Implant Stability? An In Vitro Comparative Study with Osseodensification Technique. Applied Sciences 2020, 10, 8623

2021 ◽  
Vol 11 (8) ◽  
pp. 3427
Author(s):  
Alessandro Antonelli ◽  
Francesco Bennardo ◽  
Ylenia Brancaccio ◽  
Selene Barone ◽  
Felice Femiano ◽  
...  
Keyword(s):  
Applied Sciences ◽  
Comparative Study ◽  
Implant Stability ◽  
Primary Implant

The author wishes to make the following corrections to this paper [...]

Schistosoma mansoni: a comparative study of artificially transformed schistosomula and schistomula recoverd after cercarial penetration of isolated skin

Parasitology ◽  
1977 ◽  
Vol 74 (1) ◽  
pp. 73-86 ◽  
Author(s):  
Linda H. Brink ◽  
Diane J. McLaren ◽  
S. R. Smithers
Keyword(s):  
Comparative Study ◽  
Schistosoma Mansoni ◽  
Amorphous Material ◽  
Surface Membrane ◽  
Antigenic Determinants ◽  
Agglutination Reaction ◽  
Rat Serum ◽  
Mechanical Separation ◽  
B Blood Group

A comparison was made of the ultrastructure, development and antigenic nature of the surfaces and of the viability of three types of schistosomula of Schistosoma mansoni: schistosomula formed afrer cercariae had penetrated isolated skin (SS), schistosomula produced after mechanical separation of cercarial tails from bodies (MS), and schistosomula transformed from cercariae after incubation in fresh rat serum (RS).Within 2 h of transformation, the surface membrane of all three types of schistosomula had changed from trilaminate to heptalaminate structures and SS and MS had lost their cercarial glycocalyx. Initially a dense amorphous material was demonstrated on the surfaces of RS, which was thought to be the result of an interaction between a factor in rat serum and the glycocalyx: this material was greatly reduced within 2 h of transformation. The pre-acetabular glands of SS were emptied while those of MS and RS retained their contents. Immunofluorescent studies showed that all schistosomula bound serum from mice immune to S. mansoni, but the binding was stronger with MS and RS. The mixed agglutination reaction demonstrated the presence of human A and B blood group-like antigenic determinants on approximately 30% of 3 h old SS; these determinants were not detected on MS or RS. In vitro, the development of MS and RS was similar to SS; the first schistosomula reached the ‘gut-closed’ stage by day 10; 50–70% of SS reached this stage by day 12, in contrast to only 25–50% of MS and RS. Between 28 and 45% of all schistosomula developed to maturity when injected intravenously into mice.It was concluded that the two types of artificially prepared schistosomula fultil the main criteria of transformation from cercaria to schistosomulum. Further, it is suggested that MS are the most appropriate source of material for immunochemical and physiological studies.

The effect of catecholamines and catecholamine antagonists on the third larval moult of Dirofilaria immitis in vitro

1992 ◽  
Vol 66 (4) ◽  
pp. 273-278 ◽  
Author(s):  
E. V. Warbrick ◽  
S. A. Ward
Keyword(s):  
Dirofilaria Immitis ◽  
Parasitic Nematode ◽  
The Third ◽  
Adrenergic Antagonist ◽  
Third Stage ◽  
Larval Moult

ABSTRACTVarious catecholamines and catecholamine antagonists have been examined for their effects on the third larval moult of the parasitic nematode, Dirofilaria immitis, cultured in vitro. The non-selective α and β agonist, noradrenaline, and the β agonist, isoprenaline, had no effect on the timing of the third stage moult when used at a concentration of 10−5M. The α-adrenergic antagonist. phentolamine, resulted in worm mortality at 10−5M. At 10−7M, both phentolamine and the β-antagonist, propranolol caused a significant reduction in the numbers of larvae capable of completing the third stage moult. Idazoxan, an a2-antagonist, at 10−5M did not affect worm mortality but did completely prevent ecdysis. The potential of these compounds as possible filaricides is discussed.

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