Dot-Enzyme-Linked Immunosorbent Assay (Dot-ELISA) for the Rapid Diagnosis of Human Fascioliasis

1989 ◽  
Vol 75 (4) ◽  
pp. 549 ◽  
Author(s):  
Hind I. Shaheen ◽  
Karim A. Kamal ◽  
Zoheir Farid ◽  
Noshy Mansour ◽  
Fouad N. Boctor ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Fascioliasis ◽  
Dot Elisa

scholarly journals The Diagnosis of Human Fascioliasis by Enzyme-Linked Immunosorbent Assay (ELISA) Using Recombinant Cathepsin L Protease

2013 ◽  
Vol 7 (9) ◽  
pp. e2414 ◽  
Author(s):  
Bibiana Gonzales Santana ◽  
John P. Dalton ◽  
Fabio Vasquez Camargo ◽  
Michael Parkinson ◽  
Momar Ndao
Keyword(s):  
Immunosorbent Assay ◽  
Cathepsin L ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Fascioliasis

Three Highly Sensitive and High-Throughput Serological Approaches for Detecting Dickeya dadantii in Sweet Potato

Plant Disease ◽  
2021 ◽  
Vol 105 (4) ◽  
pp. 832-839
Author(s):  
Wanqin He ◽  
Deqing Huang ◽  
Jiayu Wu ◽  
Xue Li ◽  
Yajuan Qian ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Cell Lines ◽  
Immunosorbent Assay ◽  
Root Rot ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dickeya Dadantii ◽  
Crude Extracts ◽  
Tissue Print ◽  
Hybridoma Cell Lines ◽  
Dot Elisa

Sweet potato stem and root rot is an important bacterial disease and often causes serious economic losses to sweet potato. Development of rapid and sensitive detection methods is crucial for diagnosis and management of this disease in field. Here, we report the production of four hybridoma cell lines (25C4, 16C10, 9B1, and 9H10) using Dickeya dadantii strain FY1710 as an immunogen. Monoclonal antibodies (MAbs) produced by these four hybridoma cell lines were highly specific and sensitive for D. dadantii detection. Indirect enzyme-linked immunosorbent assay (indirect-ELISA) results showed that the four MAbs 25C4, 16C10, 9B1, and 9H10 could detect D. dadantii in suspensions diluted to 4.89 × 104, 4.89 × 104, 9.78 × 104, and 9.78 × 104 CFU/ml, respectively. Furthermore, all four MAbs can react strongly and specifically with all four D. dadantii strains used in this study, not with the other seven tested bacterial strains. Using these four MAbs, three different serological approaches, triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-ELISA, and tissue-print-ELISA, were developed for detection of D. dadantii in crude extracts prepared from field-collected sweet potato plants. Among these three methods, TAS-ELISA and dot-ELISA were used to detect D. dadantii in suspensions diluted up to 1.23 × 104 and 1.17 × 106 CFU/ml, respectively, or in sweet potato crude extracts diluted up to 1:3,840 and 1:1,920 (wt/vol, grams per milliliter), respectively. Surprisingly, both TAS-ELISA and dot-ELISA serological approaches were more sensitive than the conventional PCR. Analyses using field-collected sweet potato samples showed that the newly developed TAS-ELISA, dot-ELISA, or tissue-print-ELISA were reliable in detecting D. dadantii in sweet potato tissues. Thus, the three serological approaches were highly valuable for diagnosis of stem and root rot in sweet potato production.

scholarly journals A Multi-Hemagglutinin-Based Enzyme-Linked Immunosorbent Assay to Serologically Detect Influenza A Virus Infection in Animals

2019 ◽  
Vol 6 (3) ◽  
pp. 64
Author(s):  
Miki Okumura ◽  
Akiko Takenaka-Uema ◽  
Shin Murakami ◽  
Taisuke Horimoto
Keyword(s):  
Virus Infection ◽  
Immunosorbent Assay ◽  
Influenza A ◽  
Influenza Virus Infection ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pandemic Preparedness ◽  
Influenza A Viruses ◽  
Diagnosis Method ◽  
Influenza A Virus Infection

Mammals can play a role as an intermediate host in the emergence of mammalian-adapted reassortants or mutants of avian influenza A viruses, with pandemic potential. Therefore, detecting viral infection in animals followed by assessment of the hemagglutinin (HA) subtype of the agent is an indispensable process for risk assessment in pandemic preparedness. In this study, we tested the potential of an enzyme-linked immunosorbent assay as a rapid diagnosis method, using a panel of HA subtype antigens. By analyzing reference immune sera, we found that this novel assay could detect HA subtype-specific antibodies without considerable inter-subtypic cross-reactivities, contributing to diagnosis of influenza virus infection.

Evaluation of immunoglobulin G4 subclass antibody in a peptide-based enzyme-linked immunosorbent assay for the serodiagnosis of human fascioliasis

Parasitology ◽  
2007 ◽  
Vol 134 (14) ◽  
pp. 2021-2026 ◽  
Author(s):  
C. TANTRAWATPAN ◽  
W. MALEEWONG ◽  
C. WONGKHAM ◽  
S. WONGKHAM ◽  
P. M. INTAPAN ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Large Scale ◽  
Laboratory Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Immunoglobulin G4 ◽  
Predictive Values ◽  
Diagnostic Potential ◽  
Human Fascioliasis ◽  
Specific Igg4

SUMMARYTo improve the diagnosis of human fascioliasis caused byFasciola gigantica, we developed a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA) based on the detection of specific IgG4 subclass antibody. Two identified B-cell epitopes ofF. giganticacathepsin L1 were synthesized as single synthetic peptides, acetyl-DKIDWRESGYVTELKDQGNC-carboxamide (peptide L) and acetyl-DKIDWRESGYVTEVKDQGNC-carboxamide (peptide V), and their diagnostic potential was evaluated. The sera of 25 patients infected withF. gigantica, 212 patients with other parasitic infections, 32 cholangiocarcinoma patients and 57 healthy controls were analysed. The sensitivity, specificity, accuracy, and positive and negative predictive values of this assay were the same with both peptides at 100%, 99·7%, 99·7%, 96·2% and 100%, respectively. These highly sensitive and specific peptide-based ELISAs for the detection of specific IgG4 antibody could be useful for laboratory diagnosis of human fascioliasis in future large-scale surveys throughout Southeast Asia where this disease is prevalent.

RAPID DIAGNOSIS OF TYPHOID FEVER BY ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION OF SALMONELLA SEROTYPE TYPHI ANTIGENS IN URINE

2004 ◽  
Vol 70 (3) ◽  
pp. 323-328 ◽  
Author(s):  
MOUSTAFA ABDEL FADEEL ◽  
YEHIA SULTAN ◽  
DAWLAT EL MELEGI ◽  
WILLIAM F. BIBB ◽  
BAHEIA REYAD ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Immunosorbent Assay ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salmonella Serotype ◽  
Assay Detection

SERODIAGNOSIS OF HUMAN FASCIOLIASIS BY A CYSTATIN CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY WITH RECOMBINANT FASCIOLA GIGANTICA CATHEPSIN L ANTIGEN

2005 ◽  
Vol 72 (1) ◽  
pp. 82-86 ◽  
Author(s):  
CHAIRAT TANTRAWATPAN ◽  
CHAISIRI WONGKHAM ◽  
WANCHAI MALEEWONG ◽  
KUNIO NAKASHIMA ◽  
PEWPAN M. INTAPAN ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Cathepsin L ◽  
Fasciola Gigantica ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Fascioliasis
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