Dipetalonema viteae: Survival of Adult Females and Microfilarial Release in Both a Chemically Defined and Serum-Supplemented Medium

1989 ◽  
Vol 75 (6) ◽  
pp. 953 ◽  
Author(s):  
Jun Maki ◽  
Paul P. Weinstein
Keyword(s):  
Adult Females ◽  
Dipetalonema Viteae

THE »EARLY-ANDROGEN« SYNDROME; ITS DEVELOPMENT AND THE RESPONSE TO HEMI-SPAYING

1964 ◽  
Vol 45 (1) ◽  
pp. 1-12 ◽  
Author(s):  
H. E. Swanson ◽  
J. J. van der Werff ten Bosch
Keyword(s):  
Sexual Maturity ◽  
Compensatory Growth ◽  
Testosterone Propionate ◽  
Single Injection ◽  
Weight Increase ◽  
Corpora Lutea ◽  
Transition Stage ◽  
Adult Females

ABSTRACT The »early-androgen« syndrome in the rat – i. e. anovulatory ovaries in adult females after a single injection of testosterone propionate (TP) within a week of birth – may not become apparent until some time after the attainment of sexual maturity. Large doses (50 or 100 μg) of TP were effective earlier than lower doses (5 or 10 μg). Rats which received 5 μg TP were ovulating at 10 weeks of age, mated but were infertile at 13 weeks of age, and were anovulatory at 21 weeks. In rats between 10 and 13 weeks old there was a marked fall in the number of corpora lutea in the ovaries of animals which had been given 5 μg TP. Hemi-spaying was followed by compensatory growth of the remaining ovary which consisted of corpora lutea in ovulating, and of follicles in anovulatory rats; little or no compensatory weight increase occurred in animals which seemed to be in the transition stage from the ovulatory to the anovulatory condition.

REDESCRIPTION OF HYPOASPISELLA SPICULIFER (BERLESE, 1918) COMB. N. (ACARI: MESOSTIGMATA: LAELAPIDAE) FROM SOUTH AFRICA

Acarina ◽  
2020 ◽  
Vol 28 (1) ◽  
pp. 55-64
Author(s):  
Omid Joharchi ◽  
Elizabeth Hugo-Coetzee ◽  
Sergey G. Ermilov ◽  
Alexander A. Khaustov
Keyword(s):  
South Africa ◽  
Soil Invertebrates ◽  
Current Concept ◽  
Free Living ◽  
Adult Females

Hypoaspisella spiculifer comb. n. is redescribed on the basis of adult females, collected from soil in South Africa. Hypoaspisella spiculifer fits well with the current concept of the genus Hypoaspisella Bernhard. The chelicerae of this species are similar to those of free-living mites, suggesting that it may be a predator of small soil invertebrates.

Tryptophan and Methionine Metabolism of Adult Females as Affected by Vitamin B-6 Deficiency

1974 ◽  
Vol 104 (10) ◽  
pp. 1348-1355 ◽  
Author(s):  
Heaja Kim Shin ◽  
Hellen M. Linkswiler
Keyword(s):  
Vitamin B ◽  
Methionine Metabolism ◽  
Adult Females

scholarly journals Tick Ecdysteroid Hormone, Global Microbiota/Rickettsia Signaling in the Ovary versus Carcass during Vitellogenesis in Part-Fed (Virgin) American Dog Ticks, Dermacentor variabilis

2021 ◽  
Vol 9 (6) ◽  
pp. 1242
Author(s):  
Loganathan Ponnusamy ◽  
Haley Sutton ◽  
Robert D. Mitchell ◽  
Daniel E. Sonenshine ◽  
Charles S. Apperson ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Natural Populations ◽  
Blood Feeding ◽  
Dermacentor Variabilis ◽  
Transovarial Transmission ◽  
Rickettsia Species ◽  
Adult Females ◽  
Dog Tick ◽  
Total Bacteria

The transovarial transmission of tick-borne bacterial pathogens is an important mechanism for their maintenance in natural populations and transmission, causing disease in humans and animals. The mechanism for this transmission and the possible role of tick hormones facilitating this process have never been studied. Injections of physiological levels of the tick hormone, 20-hydroxyecdysone (20E), into part-fed (virgin) adult females of the American dog tick, Dermacentor variabilis, attached to the host caused a reduction in density of Rickettsia montanensis in the carcass and an increase in the ovaries compared to buffer-injected controls. This injection initiates yolk protein synthesis and uptake by the eggs but has no effect on blood feeding. Francisella sp. and R. montanensis were the predominant bacteria based on the proportionality in the carcass and ovary. The total bacteria load increased in the carcass and ovaries, and bacteria in the genus Pseudomonas increased in the carcass after the 20E injection. The mechanism of how the Rickettsia species respond to changes in tick hormonal regulation needs further investigation. Multiple possible mechanisms for the proliferation of R. montanensis in the ovaries are proposed.

scholarly journals Biology of an Adventive Population of the Armored Scale Rhizaspidiotus donacis, a Biological Control Agent of Arundo donax in California

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 588
Author(s):  
Charles A. Braman ◽  
Adam M. Lambert ◽  
A. Zeynep Özsoy ◽  
Ellen N. Hollstien ◽  
Kirsten A. Sheehy ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Control Agent ◽  
Arundo Donax ◽  
Giant Reed ◽  
Aphytis Melinus ◽  
Adult Females ◽  
Armored Scale ◽  
Riparian Systems

Arundo donax (giant reed) is invasive in Mediterranean, sub-, and tropical riparian systems worldwide. The armored scale Rhizaspidiotus donacis is approved for biocontrol in North America, but an adventive population was recently discovered in southern California. We documented this population’s distribution, phylogeny, phenology, potential host spillover to Phragmites spp., and potential for parasitism by a common biocontrol parasitoid of citrus scale. The adventive scale was found within a single watershed and is genetically closest to Iberian scale genotypes. Rhizaspidiotus donacis developed on Phragmites haplotypes but at much lower densities than Arundo. The adventive population is univoltine, producing crawlers from March-June. Aphytis melinus parasitoids exhibited sustained interest in R. donacis during choice and no-choice trials and oviposition resulted in a small second generation. Rhizaspidiotus donacis appears limited in distribution by its univoltinism and sessile adult females. This presents challenges for broad biocontrol implementation but allows for targeted application. The genetic differentiation between imported biocontrol samples and adventive populations presents an opportunity for exploring benefits of hybrids and/or alternative genotypes where establishment has been difficult. While unlikely to occur in situ, spillover to vulnerable endemic Phragmites or deleterious parasitoid effects on scale biocontrol agents warrants consideration when planning use of R. donacis.

scholarly journals Microbiome recovery in adult females with uncomplicated urinary tract infections in a randomised phase 2A trial of the novel antibiotic gepotidacin (GSK140944)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Andrea Nuzzo ◽  
Stephanie Van Horn ◽  
Christopher Traini ◽  
Caroline R. Perry ◽  
Etienne F. Dumont ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Human Microbiome ◽  
Time Points ◽  
Pharyngeal Cavity ◽  
Adult Females ◽  
Microbiome Diversity ◽  
Antibiotic Drug ◽  
Tract Infections

Abstract Background With increasing concerns about the impact of frequent antibiotic usage on the human microbiome, it is important to characterize the potential for such effects in early antibiotic drug development clinical trials. In a randomised Phase 2a clinical trial study that evaluated the pharmacokinetics of repeated oral doses of gepotidacin, a first-in-chemical-class triazaacenaphthylene antibiotic with a distinct mechanism of action, in adult females with uncomplicated urinary tract infections for gepotidacin (GSK2140944) we evaluated the potential changes in microbiome composition across multiple time points and body-sites (ClinicalTrials.gov: NCT03568942). Results Samples of gastrointestinal tract (GIT), pharyngeal cavity and vaginal microbiota were collected with consent from 22 patients at three time points relative to the gepotidacin dosing regimen; Day 1 (pre-dose), Day 5 (end of dosing) and Follow-up (Day 28 ± 3 days). Microbiota composition was determined by DNA sequencing of 16S rRNA gene variable region 4 amplicons. By Day 5, significant changes were observed in the microbiome diversity relative to pre-dose across the tested body-sites. However, by the Follow-up visit, microbiome diversity changes were reverted to compositions comparable to Day 1. The greatest range of microbiome changes by body-site were GIT followed by the pharyngeal cavity then vagina. In Follow-up visit samples we found no statistically significant occurrences of pathogenic taxa. Conclusion Our findings suggest that gepotidacin alteration of the human microbiome after 5 days of dosing is temporary and rebound to pre-dosing states is evident within the first month post-treatment. We recommend that future antibiotic drug trials include similar exploratory investigations into the duration and context of microbiome modification and recovery. Trial registration NCT03568942. Registered 26 June 2018.

A new feeding technique for experimental and routine culturing of the insect Rhodnius prolixus

1994 ◽  
Vol 72 (12) ◽  
pp. 2244-2247 ◽  
Author(s):  
Erwin Huebner ◽  
Rene Harrison ◽  
Karen Yeow
Keyword(s):  
Cost Effective ◽  
Rhodnius Prolixus ◽  
Wire Mesh ◽  
Artificial Membrane ◽  
Egg Hatchability ◽  
Rabbit Blood ◽  
Adult Females ◽  
Feeding Technique ◽  
Heat Sealing ◽  
Membrane Envelope

We report a method for artificially feeding the reduviid insect Rhodnius prolixus that utilizes heparinized rabbit blood enclosed in an artificial-membrane envelope. Envelopes of Parafilm M® or Nescofilm® are made by heat sealing two pieces, one of which is stretched first, on three sides. Blood is then put into the envelope, the air expelled, and the fourth side sealed with a bag sealer. The blood-filled envelopes are then placed on a standard slide warmer set at 37 °C and insects in jars with wire-mesh tops are allowed to feed. Normal gorging and subsequent molting of instars were attained. Adult females show normal fecundity (the E value was 0.19 for mated females) and egg hatchability was also normal. The method is simple, cost effective, and easy to implement. It is especially suited for experimental feedings.

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