Light and Scanning Electron Microscopy of the Ecdysis of Haemonchus contortus Infective Larvae

1989 ◽  
Vol 75 (2) ◽  
pp. 303 ◽  
Author(s):  
H. R. Gamble ◽  
J. R. Lichtenfels ◽  
J. P. Purcell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Infective Larvae ◽  
Scanning Electron

Scanning electron microscopy of different vulval structures in a Mexican Haemonchus contortus isolate

2021 ◽  
pp. 100640
Author(s):  
Cintli Martínez-Ortiz-de-Montellano ◽  
Juan Felipe de Jesús Torres-Acosta ◽  
Carlos Alfredo Sandoval-Castro ◽  
Isabelle Fourquaux ◽  
Hervé Hoste
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Scanning Electron

scholarly journals Nematicidal Effect and Histological Modifications Induced by Hydrolysable Tannin Extract on the Third-Stage Infective Larvae of Haemonchus contortus

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 442
Author(s):  
Perla María del Carmen Acevedo-Ramírez ◽  
Claudia Hallal-Calleros ◽  
Iván Flores-Pérez ◽  
Fernando Alba-Hurtado ◽  
María Berenit Mendoza-Garfias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Effective Control ◽  
Tannin Extract ◽  
Physical Damage ◽  
Hydrolysable Tannin ◽  
Green Production ◽  
Scanning Electron

Haemonchus contortus is the most frequent and most important nematode parasite in the ruminants production of tropical and subtropical regions. There are strains resistant to all families of available anthelmintics. Consequently, the conduction of research to find other resources that allow effective control of this parasitic disease, preferably focusing on green production, is necessary. The aim of this study was to evaluate the effect of hydrolysable tannin extract (HTE) on larvae 3 (L3) of H. contortus in vitro. L3 were exposed to different HTE concentrations and times. In addition, both light and scanning electron microscopy were used to explore physical damage on L3 subjected to HTE activity. After 72 h of incubation, the mean lethal concentration of HTE was 2 mg/mL; this concentration has been previously referred to as safe for consumption in cattle. Scanning electron microscopy revealed H. contortus L3 destruction, damage was evident by separation of the sheath mainly in the cervical and caudal regions of the larva and by rupture of the cuticle with larval evisceration. Our results suggest that hydrolysable tannin extract from chestnut could be considered as a green alternative for parasitic control in ruminants.

Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions

2013 ◽  
Vol 133 (3) ◽  
pp. 281-286 ◽  
Author(s):  
C. Martínez-Ortíz-de-Montellano ◽  
C. Arroyo-López ◽  
I. Fourquaux ◽  
J.F.J. Torres-Acosta ◽  
C.A. Sandoval-Castro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Scanning Electron

scholarly journals Interaction between the nematophagous fungusDuddingtonia flagransand infective larvae ofHaemonchus contortus(Nematoda: Trichostrongyloidea)

2008 ◽  
Vol 82 (4) ◽  
pp. 337-341 ◽  
Author(s):  
A.K. Campos ◽  
J.V. Araújo ◽  
M.P. Guimarães
Keyword(s):  
Electron Microscopy ◽  
Haemonchus Contortus ◽  
Scanning Electron Micrographs ◽  
Duddingtonia Flagrans ◽  
Larval Cuticle ◽  
Infective Larvae ◽  
Dialysis Membranes ◽  
Nematode Cuticle ◽  
Scanning Electron

AbstractThe interaction betweenDuddingtonia flagransand infective larvae ofHaemonchus contortuswas studiedin vitrounder optical and scanning electron microscopy. Trap formation by the fungus started 9 hours after inoculation and first larvae were found 11 hours after larval inoculation on colonies grown on the surface of dialysis membranes. Scanning electron micrographs were taken 12, 24, 36 and 48 h after larval predation. Details of predation structures and fungus–larvae interaction are described. A mucilaginous substance occurred at the points of adherence of traps to nematode cuticle. Bacteria were also found at some points of interaction between fungus and larval cuticle. Cuticle penetration by fungus hyphae occurred only 48 h after predation.

Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus

Parasitology ◽  
1985 ◽  
Vol 90 (2) ◽  
pp. 357-363 ◽  
Author(s):  
C. D. Nicholls ◽  
D. L. Lee ◽  
M. J. Sharpe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Parasitic Nematodes ◽  
Fibre Optic ◽  
Biopsy Specimens ◽  
Optic Endoscopy ◽  
Surrounding Areas ◽  
Scanning Electron

The abomasum of living sheep infected with 50000 larvae of Haemonchus contortus was examined before and during infection, by means of fibre optic endoscopy. Biopsy specimens were removed from the abomasum of the living sheep and were examined by means of scanning electron microscopy. Changes were noted in the surface structure of the abomasum 2 days after infection and larvae were seen on, and burrowing into, the mucosa. These changes became more pronounced as the infection proceeded, especially after day 10 of the infection when the adult worms had appeared. The behaviour of adult nematodes was observed within the abomasum and on occasions they were seen to move from surrounding areas into an area of haemorrhage, caused by removal of a biopsy specimen. Individual nematodes were removed from the abomasum by means of the biopsy forceps. Fibre optic endoscopy was shown to be a useful tool in the study of parasitic nematodes in vivo.

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