Serum-Stimulated Feeding In vitro by Third-Stage Infective Larvae of the Canine Hookworm Ancylostoma caninum

1990 ◽  
Vol 76 (3) ◽  
pp. 394 ◽  
Author(s):  
J. M. Hawdon ◽  
G. A. Schad
Keyword(s):  
Ancylostoma Caninum ◽  
Infective Larvae ◽  
Third Stage

In vitro trapping capability of Arthrobotrys spp. on infective larvae of Haemonchus contortus and Nacobbus aberrans

1994 ◽  
Vol 68 (3) ◽  
pp. 223-229 ◽  
Author(s):  
P. Mendoza-de Gives ◽  
E. Zavaleta-Mejia ◽  
D. Herrera-Rodriguez ◽  
H. Quiróz-Romero
Keyword(s):  
Microscopic Examination ◽  
Haemonchus Contortus ◽  
Three Dimensional ◽  
Arthrobotrys Oligospora ◽  
Second Stage ◽  
Infective Larvae ◽  
Third Stage ◽  
Nacobbus Aberrans

AbstractThe trapping capability of Arthrobotrys oligospora and A. conoides (Hyphomycetales) against third stage larvae (L3) of Haemonchus contortus (Trichostrongylidae) was evaluated in an in vitro trial. Arthrobotrys oligospora showed a 35.87% and 25.71% trapping effectiveness against H. contortus infective larvae at 18 and 25°C, respectively; whereas the trapping capability of A. conoides was 92.17% and 90.40% at the same temperatures, respectively. Microscopic examination demonstrated that A. conoides spontaneously developed a large quantity of three-dimensional loops before the nematodes were added. Neither of the two species studied developed three-dimensional adhesive loops at 30°C, consequently no trapped nematode was observed. In a second trial, the trapping capability of A. conoides against H. contortus (L3) and second stage larvae (J2) of Nacobbus aberrans (Pratylenchidae), was evaluated at 25°C. The trapping capability shown by A. conoides was higher than 90% for both kinds of nematode. The possible use of A. conoides to control ovine haemonchosis is discussed.

Studies with Brugia pahangi 12. The activity of levamisole

1976 ◽  
Vol 50 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Rosemary Rogers ◽  
D. A. Denham
Keyword(s):  
Aedes Aegypti ◽  
Developmental Stages ◽  
Brugia Pahangi ◽  
Infective Larvae ◽  
Vector Mosquito ◽  
Third Stage ◽  
Dose Levels

AbstractThe effects of levamisole on adults, third stage infective larvae, and microfilariae of Brugia pahangi were studied in in vitro culture and in vivo against developing stages in the vector mosquito and in infected cats. In vitro the drug was effective only at dose levels much higher than can be tolerated by mammals. It was active against the developmental stages of the worm in the vector Aedes aegypti.The drug was strongly microfilaricidal in cats but less effective against adult worms.

scholarly journals In vitro chemotactic responses of Brugia pahangi infective larvae to sodium ions

2011 ◽  
Vol 86 (4) ◽  
pp. 406-409 ◽  
Author(s):  
Y. Mitsui ◽  
M. Miura ◽  
D.A. Bome ◽  
Y. Aoki
Keyword(s):  
Deionized Water ◽  
Sodium Ions ◽  
Brugia Pahangi ◽  
Low Concentration ◽  
Infective Larvae ◽  
Significant Tendency ◽  
Third Stage

AbstractIn vitro chemotactic responses of infective third-stage larvae (L3) of Brugia pahangi to NaCl, Na2HPO4, KCl, K2HPO4, MgCl2 and CaCl2 were assessed. Compared to deionized water as a control, 200 mm NaCl and 100 mm Na2HPO4 significantly attracted L3 (P < 0.01 and P < 0.01), whereas L3 were likely to avoid 200 mm KCl and 100 mm K2HPO4 (P < 0.05 and P < 0.05). L3 showed no significant tendency to avoid or to be attracted to 200 mm CaCl2 and 200 mm MgCl2. Furthermore, NaCl exhibited a significant chemoattractant activity for L3 at a low concentration of 100 mm.

scholarly journals Condensed tannins and gastro-intestinal parasites in sheep

1999 ◽  
pp. 57-61 ◽  
Author(s):  
A.L. Molan ◽  
G.C. Waghorn ◽  
W.C. Mcnabb
Keyword(s):  
Condensed Tannins ◽  
Intestinal Parasites ◽  
In Vitro Assays ◽  
Trichostrongylus Colubriformis ◽  
Egg Hatching ◽  
Infective Larvae ◽  
Third Stage ◽  
Principal Method

As the effectiveness of current anthelmintic drenches is being reduced by the emergence of drench resistance and significant production losses may still occur as a result of continuing larval challenge, strategies for nematode control should focus on preventing the build-up of infective larvae on pasture rather than treating the infection. This paper reports the effects of condensed tannins (CT) extracted from Lotus pedunculatus, Lotus corniculatus, sulla (Hedysarum coronarium) and sainfoin (Onobrychus viciifolia) on in vitro viability of eggs, first stage larvae (L1) and third stage larvae (L3) of the sheep nematode, Trichostrongylus colubriformis. When in vitro assays contained CT in a similar concentrations to that in the digestive tract of sheep fed these forages, the CT reduced the development of eggs to L3 larvae as well as the proportion of eggs hatching. The CT also reduced the development of L1 larvae to L3 larvae and decreased the motility of L3 larvae when assessed by the larval migration inhibition assay and this may reduce their infective capacity in vivo. If these forages are fed to grazing sheep, then it may be possible to reduce contamination of pasture with infective larvae and reduce our dependence on anthelmintics as the principal method for controlling internal parasites. Keywords: condensed tannins, drench resistance, egg hatching, larval development, Lotus, parasites, sainfoin, sulla, Trichostrongylus colubriformis

Sign in / Sign up

Export Citation Format

Share Document