Purine Base and Nucleoside Uptake in Plasmodium berghei and Host Erythrocytes

1980 ◽  
Vol 66 (2) ◽  
pp. 205 ◽  
Author(s):  
Brian D. Hansen ◽  
H. Kenneth Sleeman ◽  
Peter W. Pappas
Keyword(s):  
Plasmodium Berghei ◽  
Purine Base ◽  
Nucleoside Uptake

The specificity of purine base and nucleoside uptake in promastigotes ofLeishmania braziliensis panamensis

Parasitology ◽  
1982 ◽  
Vol 85 (2) ◽  
pp. 271-282 ◽  
Author(s):  
B. D. Hansen ◽  
J. Perez-Arbelo ◽  
J. F. Walkony ◽  
L. D. Hendricks
Keyword(s):  
Competitive Interactions ◽  
Leishmania Braziliensis ◽  
Purine Base ◽  
Purine Bases ◽  
Uptake Rates ◽  
Nucleoside Uptake ◽  
And Diffusion

SUMMARYPromastigotes ofLeishmania braziliensis panamensisabsorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. Competitive interactions among those purines tested confirmed the presence of mediated and diffusion components and suggested that three transport loci may be operating (Fig. 6). The first transport locus, designated Locus 1, transported inosine, Locus 2, the purine bases hypoxanthine and adenine and Locus 3, adenosine. In addition, adenine and hypoxanthine inhibited the uptake of one another competitively. A comparison of Kivalues derived from double reciprocal plots of labelled hypoxanthine and adenine uptake in the presence of the unlabelled substrates as inhibitors suggested that adenine has a greater affinity for the transport locus.

Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

2019 ◽  
Vol 3 (3) ◽  
pp. 80-84
Author(s):  
Amina Olorukooba ◽  
◽  
Ferhat Khan ◽  
Charles Madu ◽  
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity

Ginger Extract Has An Ability To Safe C57BL / 6-WT Mice From Cerebral Malaria Produced By Plasmodium Berghei ANKA

2018 ◽  
pp. 1
Author(s):  
سالم رمضان على السريتى ◽  
نوارة امراجع الازيرق العمارى
Keyword(s):  
Cerebral Malaria ◽  
Plasmodium Berghei ◽  
Plasmodium Berghei Anka ◽  
Ginger Extract

In Vitro Effect of Chlorquine and Picroliv on Plasmodium Berghei Induced Alterations in the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture

2020 ◽  
Vol 20 ◽  
Author(s):  
Anchal Trivedi ◽  
Aparna Misra ◽  
Esha Sarkar ◽  
Anil K. Balapure
Keyword(s):  
Drug Resistance ◽  
Plasmodium Berghei ◽  
Adenosine Triphosphatase ◽  
Explant Culture ◽  
Need To Evaluate ◽  
Mda Content ◽  
High Level ◽  
Vitro Effect ◽  
Positive Effect

Background: In recent years, great progress has been made in reducing the high level of malaria suffering worldwide. There is a great need to evaluate drug resistance reversers and consider new medicines against malaria. There are many approaches to the development of antimalarial drugs. Specific concerns must be taken in to account in these approaches, in particular there requirement for very in expensive and simple use of new therapies and the need to limit drug discovery expenses. Important ongoing efforts are the optimisation of treatment with available medications, including the use of combination therapy. The production of analogs of known agents and the identification of natural products, the use of compounds originally developed against other diseases, the assessment of overcoming drug resistance and the consideration of new therapeutic targets. Liver and spleen are the important organs which are directly associated with malarial complications. Aim: An analysis the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture. Objective: To determine in-Vitro Effect of Chlorquine and Picroliv on Plasmodium Berghei Induced Alterations in the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and Malondialdehyde in spleen Explant Culture. Material and method: 1-Histological preparation of spleen explants for paraplast embedding 2-Biochemicalstudies (Enzymes (Atpase, ALP&GST) and the level of protein, Malondialdehyde (MDA). Result: Splenomegalyis one of the three main diagnostic parameters of malaria infection besides fever and anaemia. Many enzymes present in the liver and spleen may also be altered or liberated under different pathological conditions. Enzymes (ATPase, ALP&GST) and the level of protein, Malondialdehyde (MDA) content was found to increase in the liver and spleen explants during malarial infection. In the liver and spleen derived from parasitized CQ treated animals, the activity of all the above enzymes (ATPase, ALP&GST) and the level of protein & MDA of liver/spleen reversed towards the normal for all the 4or3 days of incubations. Picroliv efficacy decreased with the increment of parasitaemia and at 60%parasitaemia. Conclusion: Alkalinephosphatase (ALP) was found to increase with increasing parasitaemia. After the addition of Picroliv to the medium, a decrement in the activity was observed up to day 4 of culture.A similar positive effect of Picroliv was observed on the ATPase and ALP activity of spleen explants.DNA and protein contents also increased in the parasitized liver cultured in the presence of picroliv.On the contrary, in the spleen explants DNA, protein and MDA content were found to decrease after Picroliv supplementation to the culture medium.

Plasmodium berghei Infections in Gnotobiotic Mice and Rats: Parasitologic, Immunologic and Histopathologic Observations

1966 ◽  
Vol 131 (suppl_9) ◽  
pp. 870-890 ◽  
Author(s):  
L. K. Martin ◽  
A. Einheber ◽  
R. F. Porro ◽  
E. H. Sadun ◽  
H. Bauer
Keyword(s):  
Plasmodium Berghei ◽  
Gnotobiotic Mice

Safety assessment and antimalarial property of methanol extract of Fagara zanthoxyloides root-bark on Plasmodium berghei-infected mice

2021 ◽  
Author(s):  
Christian Chijioke Amah ◽  
Osmund Chukwuma Enechi ◽  
Daniel Emmanuel Ekpo ◽  
Innocent Uzochukwu Okagu ◽  
Chidinma Pamela Ononiwu ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Safety Assessment ◽  
Methanol Extract ◽  
Root Bark

scholarly journals Dexamethasone increased the survival rate in Plasmodium berghei-infected mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Danilo Reymão Moreira ◽  
Ana Carolina Musa Gonçalves Uberti ◽  
Antonio Rafael Quadros Gomes ◽  
Michelli Erica Souza Ferreira ◽  
Aline da Silva Barbosa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Survival Rate ◽  
Plasmodium Berghei ◽  
Redox Status ◽  
No Synthase ◽  
Ischemia And Reperfusion ◽  
Inos Inhibition ◽  
The Brain ◽  
Plasmodial Infection ◽  
Stimulation Of

AbstractThe present study aimed to evaluate the effects of dexamethasone on the redox status, parasitemia evolution, and survival rate of Plasmodium berghei-infected mice. Two-hundred and twenty-five mice were infected with Plasmodium berghei and subjected to stimulation or inhibition of NO synthesis. The stimulation of NO synthesis was performed through the administration of L-arginine, while its inhibition was made by the administration of dexamethasone. Inducible NO synthase (iNOS) inhibition by dexamethasone promoted an increase in the survival rate of P. berghei-infected mice, and the data suggested the participation of oxidative stress in the brain as a result of plasmodial infection, as well as the inhibition of brain NO synthesis, which promoted the survival rate of almost 90% of the animals until the 15th day of infection, with possible direct interference of ischemia and reperfusion syndrome, as seen by increased levels of uric acid. Inhibition of brain iNOS by dexamethasone caused a decrease in parasitemia and increased the survival rate of infected animals, suggesting that NO synthesis may stimulate a series of compensatory redox effects that, if overstimulated, may be responsible for the onset of severe forms of malaria.

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