Trichinella spiralis: Partial Characterization of Antigens Isolated by Immuno-Affinity Chromatography from the Large-Particle Fraction of the Muscle Larvae

1981 ◽  
Vol 67 (3) ◽  
pp. 332 ◽  
Author(s):  
Dickson D. Despommier ◽  
Anthony Laccetti
Keyword(s):  
Affinity Chromatography ◽  
Large Particle ◽  
Trichinella Spiralis ◽  
Partial Characterization ◽  
Particle Fraction ◽  
Muscle Larvae

scholarly journals Cellular and molecular changes and immune response in the intestinal mucosa during Trichinella spiralis early infection in rats

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
María Priscila Saracino ◽  
Cecilia Celeste Vila ◽  
Melina Cohen ◽  
María Virginia Gentilini ◽  
Guido Hernán Falduto ◽  
...  
Keyword(s):  
Immune Response ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Mucosal Immune Response ◽  
Mesenteric Lymph Nodes ◽  
Muscle Larvae ◽  
Gut Immunity ◽  
Gut Mucosa

Abstract Background: The main targets of the host’s immune system in Trichinella spiralis infection are the adult worms (AW), at the gut level, and the migrant or newborn larvae (NBL), at systemic and pulmonary levels. Most of the studies carried out in the gut mucosa have been performed on the Payer’s patches and/or the mesenteric lymph nodes but not on the lamina propria, therefore, knowledge on the gut immune response against T. spiralis remains incomplete. Methods This study aimed at characterizing the early mucosal immune response against T. spiralis, particularly, the events taking place between 1 and 13 dpi. For this purpose, Wistar rats were orally infected with muscle larvae of T. spiralis and the humoral and cellular parameters of the gut immunity were analysed, including the evaluation of the ADCC mechanism exerted by lamina propria cells. Results A marked inflammation and structural alteration of the mucosa was found. The changes involved an increase in goblet cells, eosinophils and mast cells, and B and T lymphocytes, initially displaying a Th1 profile, characterised by the secretion of IFN-γ and IL-12, followed by a polarization towards a Th2 profile, with a marked increase in IgE, IgG1, IL-4, IL-5 and IL-13 levels, which occurred once the infection was established. In addition, the helminthotoxic activity of lamina propria cells demonstrated the role of the intestine as a place of migrant larvae destruction, indicating that not all the NBLs released in the gut will be able to reach the muscles. Conclusions The characterization of the immune response triggered in the gut mucosa during T. spiralis infection showed that not only an effector mechanism is directed toward the AW but also towards the NBL as a cytotoxic activity was observed against NBL exerted by lamina propria cells.

scholarly journals Characterization of a Novel Glutamine Synthetase From Trichinella spiralis and Its Participation in Larval Acid Resistance, Molting, and Development

2021 ◽  
Vol 9 ◽  
Author(s):  
Tong Xu Zhuo ◽  
Zhen Wang ◽  
Yan Yan Song ◽  
Shu Wei Yan ◽  
Ruo Dan Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Life Cycle ◽  
Glutamine Synthetase ◽  
Enzymatic Activity ◽  
Post Infection ◽  
Catalytic Domain ◽  
Trichinella Spiralis ◽  
Acid Resistance ◽  
Muscle Larvae

Trichinella spiralis is a major foodborne parasite worldwide. After the encapsulated muscle larvae (ML) in meat are ingested, the ML are liberated in the stomach of the host and activated into intestinal infectious larvae (IIL), which develop into adult worm after molting four times. A novel glutamine synthetase (TsGS) was identified from T. spiralis IIL at 10 h post-infection, but its biological role in T. spiralis life cycle is not clear. The aim of this study was to investigate the biological characteristics of TsGS and its functions in larval acid resistance, molting, and development. TsGS has a glutamine synthetase (GS) catalytic domain. Complete TsGS sequence was cloned and expressed in Escherichia coli BL21. rTsGS has good immunogenicity. qPCR and Western blotting showed that TsGS was highly expressed at IIL stage, and immunofluorescence revealed that TsGS was principally localized at the cuticle and intrauterine embryos of this nematode. rTsGS has enzymatic activity of natural GS to hydrolyze the substrate (Glu, ATP, and NH4+). Silencing of TsGS gene significantly reduced the IIL survival at pH 2.5, decreased the IIL burden, and impeded larval molting and development. The results demonstrated that TsGS participates in T. spiralis larval acid resistance, molting and development, and it might be a candidate vaccine target against Trichinella molting and development.

scholarly journals Purification and partial characterization of cathepsin D from porcine (Sus scrofa) liver using affinity chromatography

IUBMB Life ◽  
1998 ◽  
Vol 45 (4) ◽  
pp. 797-803 ◽  
Author(s):  
Femanda Canduri ◽  
Richard Ward ◽  
Walter de Azevedo ◽  
Roseli Gomes ◽  
R. K. Arni
Keyword(s):  
Affinity Chromatography ◽  
Cathepsin D ◽  
Sus Scrofa ◽  
Partial Characterization

Characterization of Spanish Trichinella isolates by random amplified polymorphic DNA (RAPD)

1996 ◽  
Vol 70 (4) ◽  
pp. 335-343 ◽  
Author(s):  
E. Rodríguez ◽  
J. Nieto ◽  
J.A. Castillo ◽  
T. Gárate
Keyword(s):  
Copy Number ◽  
Rapd Markers ◽  
Trichinella Spiralis ◽  
Random Amplified Polymorphic Dna ◽  
Arbitrary Primers ◽  
Cross Hybridization ◽  
Muscle Larvae ◽  
Genetic Clusters ◽  
Species Specific

AbstractThe random amplified polymorphic DNA (RAPD) assay was used to find molecular markers able to distinguish Trichinella spiralis from T. britovi, the two recognized Spanish Trichinella species. Fourteen Spanish Trichinella isolates, as well as reference Trichinella isolates representing the five species T. spiralis (T1), T. nativa (T2), T. britovi (T3), T. pseudospiralis (T4) and T. nelsoni (T7) and the three other taxa Trichinella T5, Trichinella T6 and Trichinella T8 of the genus, were characterized by RAPD using both purified and crude DNAs from infective muscle larvae (ML) and seven arbitrary primers. Three primers yielded diagnostic RAPD markers for the Spanish T. spiralis and T. britovi isolates as well as for the Trichinella reference isolates analysed, and in the case of crude DNAs the results were obtained in few hours. In addition, the species-specificity of the diagnostic RAPD markers from Spanish Trichinella isolates was studied by cross-hybridization assays. These assays confirmed that the selected diagnostic DNA fragments were not species-specific, but showed potential differences in the copy number among the examined Trichinella genetic clusters.

Partial characterization of two antigens secreted by L1 larvae of Trichinella spiralis

1990 ◽  
Vol 41 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Allen M. Gold ◽  
Dickson D. Despommier ◽  
Stephen W. Buck
Keyword(s):  
Trichinella Spiralis ◽  
Partial Characterization

scholarly journals Influence of chemically and biosynthesized silver nanoparticles on in vitro viability and infectivity of Trichinella spiralis muscle larvae

2021 ◽  
Author(s):  
ahmed kamal dyab ◽  
Salwa Mahmoud Abd-ELrahman ◽  
Abeer El-sayed Mahmoud ◽  
Nahed Ahmed Elossily ◽  
Fahd mohammed Alsharif ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
In Vitro Study ◽  
Transmission Electron ◽  
Muscle Larvae ◽  
Principal Finding ◽  
Vitro Model ◽  
Biosynthesized Agnps ◽  
Vitro Effect

Background Trichinellosis is a serious worldwide parasitic zoonosis. The available therapy for the treatment of Trichinella spiralis is not satisfactory. This work aimed at evaluating of the in vitro effect of silver Therefore, the recovery of effective treatment is required.nanoparticles (AgNPs) on muscle larvae of Trichinella. Methodology / principal finding The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of T. spiralis. The used AgNPs were chemically prepared using NaBH4 as reducing agent and biosynthesized using methanolic myrrh extract. Characterization of synthesized AgNPs was monitored via UV-Vis spectrophotometry, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) studies. The ML incubated with AgNPs at concentrations ranged from 1μg/ml to 20μg/ml. Conclusions /Significance Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of Trichinella. Additionally, this in vitro study showed degenerative changes  affecting the cuticle of AgNPs treated ML. The effectiveness of AgNPs on the infectivity of Trichinella ML was also assessed. The results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models. This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic activity against Trichinella in an in vitro model.

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