Axenic Culture of Trypanosoma cruzi in a Chemically Defined Medium

1975 ◽  
Vol 61 (1) ◽  
pp. 144 ◽  
Author(s):  
Scoff J. Anderson ◽  
Stuart M. Krassner
Keyword(s):  
Trypanosoma Cruzi ◽  
Axenic Culture ◽  
Defined Medium ◽  
Chemically Defined Medium

Physiological ecology of the bloom-forming alga Chrysochromulina breviturrita (Prymnesiophyceae) from lakes influenced by acid precipitation

1985 ◽  
Vol 63 (12) ◽  
pp. 2231-2239 ◽  
Author(s):  
John D. Wehr ◽  
Lewis M. Brown ◽  
Kathryn O'Grady
Keyword(s):  
Axenic Culture ◽  
Lake Ontario ◽  
Acid Precipitation ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Tolerance Range ◽  
Northeastern North America ◽  
Ph Tolerance ◽  
Average Water ◽  
Nutritional Studies

Many soft-water lakes in northeastern North America subject to acid precipitation have been affected by lakewide odours. These odours are associated with blooms consisting of nearly unialgal populations of the greenish gold alga Chrysochromulina breviturrita Nich. (Prymnesiophyceae). The alga was isolated from Cinder Lake (Ontario) into axenic culture and maintained in a chemically defined medium which corresponds to the chemistry of lakes influenced by acid precipitation. The medium which was developed, Muskoka No. 42, is described in detail and compared with the average water chemistry of affected lakes. The pH tolerance range of C. breviturrita was determined to be 4.0–6.9, with an optimum of 5.5–6.9; the alga was unable to survive above neutrality. Additions of [Formula: see text] at 100 μM or greater completely inhibited growth. Nutritional studies suggested an inability to grow on any nitrogen source other than [Formula: see text]. The alga was also found to require the vitamins B12 and thiamine but not biotin. The present results represent the first published study in which a freshwater member of the Prymnesiophyceae has been maintained axenically in a defined medium.

Improved cryopreservation of domestic cat sperm in a chemically defined medium

2012 ◽  
Vol 78 (9) ◽  
pp. 2120-2128 ◽  
Author(s):  
M.M. Vick ◽  
H.L. Bateman ◽  
C.A. Lambo ◽  
W.F. Swanson
Keyword(s):  
Defined Medium ◽  
Domestic Cat ◽  
Chemically Defined Medium

scholarly journals In vitro mouse spermatogenesis with an organ culture method in chemically defined medium

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192884 ◽  
Author(s):  
Hiroyuki Sanjo ◽  
Mitsuru Komeya ◽  
Takuya Sato ◽  
Takeru Abe ◽  
Kumiko Katagiri ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Defined Medium ◽  
Chemically Defined Medium

scholarly journals Axenic Culture of the Heterotrophic DinoflagellatePfiesteria shumwayaein a Semi-Defined Medium

2009 ◽  
Vol 56 (1) ◽  
pp. 73-82 ◽  
Author(s):  
HAYLEY M. SKELTON ◽  
JOANN M. BURKHOLDER ◽  
MATTHEW W. PARROW
Keyword(s):  
Axenic Culture ◽  
Defined Medium
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