In vitro Culture of the Vector Phase of Snake Hemogregarines in Mosquito Cell Lines

1972 ◽  
Vol 58 (1) ◽  
pp. 148 ◽  
Author(s):  
Jowett Chao ◽  
Gordon H. Ball
Keyword(s):  
In Vitro Culture ◽  
Cell Lines ◽  
Mosquito Cell ◽  
Mosquito Cell Lines

scholarly journals Adventitious viruses persistently infect three commonly used mosquito cell lines

2018 ◽  
Author(s):  
James Weger-Lucarelli ◽  
Claudia Rückert ◽  
Nathan D. Grubaugh ◽  
Michael J. Misencik ◽  
Philip M. Armstrong ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rna Virus ◽  
Mosquito Cell ◽  
Double Stranded Rna ◽  
Active Viral Replication ◽  
Mosquito Cell Lines ◽  
Persistently Infect ◽  
Generation Sequencing ◽  
Cellular Genome

AbstractMosquito cell lines were first established in the 1960’s and have been used extensively in research to isolate and propagate arthropod-borne (arbo-) viruses, study the invertebrate immune system, and understand virus-vector interactions. Despite their utility as anin vitrotool, these cell lines are poorly defined and may harbor insect-specific viruses that could impact experimental results. Accordingly, we screened four commonly-used mosquito cell lines, C6/36 and U4.4 cells fromAedes albopictus, Aag2 cells fromAedes aegypti, and Hsu cells fromCulex quinquefasciatus, for the presence of adventitious viruses. All four cell lines stained positive for double-stranded RNA by immunofluorescence, indicative of RNA virus replication. We subsequently identified viruses infecting Aag2, U4.4 and Hsu cell lines using untargeted next-generation sequencing, but not C6/36 cells. Sequences from viruses in the familiesBirnaviridae,Bunyaviridae, Flaviviridae,andRhabdoviridaewere abundant in the mosquito cell lines. PCR confirmation revealed that these sequences stem from active viral replication and/or integration into the cellular genome. Our results show that these commonly-used mosquito cell lines are persistently-infected with several viruses. This finding may be critical to interpreting data generated in these systems.

MP55-20 ESTABLISHMENT OF LUCAP CELL LINES BY “ORGANOID” IN VITRO CULTURE TECHNOLOGY

2015 ◽  
Vol 193 (4S) ◽  
Author(s):  
Catherine Dowling ◽  
Dong Gao ◽  
Samuel Kaffenberger ◽  
Youxin Guan ◽  
Qi Fan Zhang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Cell Lines ◽  
Culture Technology

scholarly journals Aedes aegypti (Aag2)-derived clonal mosquito cell lines reveal the effects of pre-existing persistent infection with the insect-specific bunyavirus Phasi Charoen-like virus on arbovirus replication

2019 ◽  
Vol 13 (11) ◽  
pp. e0007346 ◽  
Author(s):  
Anthony C. Fredericks ◽  
Tiffany A. Russell ◽  
Louisa E. Wallace ◽  
Andrew D. Davidson ◽  
Ana Fernandez-Sesma ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cell Lines ◽  
Persistent Infection ◽  
Mosquito Cell ◽  
Mosquito Cell Lines

The Expression of CD56 Is Frequently Accompanied with the Expression of Neuronal Cell Markers and Its Down-Regulation Is Induced by IL-6 in Human Myeloma Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3534-3534
Author(s):  
Mohd S. Iqbal ◽  
Ken-ichiro Otsuyama ◽  
Karim Shamsasenjan ◽  
Saeid Abroun ◽  
Jakia Amin ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Cell Lines ◽  
Cell Lineage ◽  
Neuronal Cell ◽  
Myeloma Cell ◽  
Myeloma Cells ◽  
Cell Markers ◽  
Phenotypic Changes ◽  
Human Myeloma Cells

Abstract Human myeloma cells have the marked phenotypic heterogeneity of surface marker expressions, possibly because of loss of PAX-5 expression. Especially, ectopic expression of CD56, one of non-B cell lineage markers, is frequently detected on primary myeloma cells from more than 80% patients with overt myeloma. However, only 2 (NOP2 and AMO1) out of 10 myeloma cell lines were CD56(+). In primary myeloma cells as well as CD56(−) myeloma cell lines, the treatment with forskolin could induce the expression of CD56 in the in vitro culture. In most CD56(+) primary myeloma cells as well as myeloma cell lines, the expressions of neuronal cell markers such as neuron specific enolase (NSE), nestin, β-tubulin III or chromogranin A were found coincidentally. By gene expression profiling, CD56(+) myeloma cell lines showed the marked expressions of transcription factors involved in neuronal cell lineage. On the other hand, addition of IL-6 down-regulated the expression of CD56 in CD56(+) myeloma cell lines in the in vitro culture. In 13 out of 60 patients with overt myeloma, these myeloma cells showed CD56(−) and their values of plasma CRP were significantly increased and MPC-1(−)CD45(+) immature myeloma cells were also increased compared to those in CD56(+) myeloma cases. Therefore, these results indicate that the expression of CD56 is possibly due to phenotypic changes into neuronal cell lineage, and IL-6 can block these phenotypic changes, keeping PAX-5(−) myeloma cells being uncommitted cells to any lineage.

Stable transformation of mosquito cell lines using a hsp70::neo fusion gene

Gene ◽  
1993 ◽  
Vol 136 (1-2) ◽  
pp. 129-136 ◽  
Author(s):  
Gareth J. Lycett ◽  
Julian M. Crampton
Keyword(s):  
Cell Lines ◽  
Fusion Gene ◽  
Stable Transformation ◽  
Mosquito Cell ◽  
Mosquito Cell Lines
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