Characterization of Deoxyribonucleic Acid of Entamoeba histolytica by Cesium Chloride Density Centrifugation

1971 ◽  
Vol 57 (5) ◽  
pp. 939 ◽  
Author(s):  
Richard E. Reeves ◽  
Terrie S. Lushbaugh ◽  
Francisco E. Montalvo
Keyword(s):  
Entamoeba Histolytica ◽  
Deoxyribonucleic Acid ◽  
Cesium Chloride ◽  
Density Centrifugation

Characterization of the Deoxyribonucleic Acid of Representative Strains of Entamoeba histolytica, E. histolytica-like Amebae, and E. moshkovskii

1971 ◽  
Vol 57 (4) ◽  
pp. 906 ◽  
Author(s):  
Albert H. Gelderman ◽  
David B. Keister ◽  
I. Louise Bartgis ◽  
Louis S. Diamond
Keyword(s):  
Entamoeba Histolytica ◽  
Deoxyribonucleic Acid

scholarly journals Characterization of Herpes Simplex Virus-induced Deoxyribonucleic Acid Polymerase

1973 ◽  
Vol 248 (18) ◽  
pp. 6270-6277
Author(s):  
Arthur Weissbach ◽  
Su-Chen L. Hong ◽  
Jayme Aucker ◽  
Robert Muller
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Deoxyribonucleic Acid ◽  
Simplex Virus

scholarly journals Functional Characterization of Spliceosomal Introns and Identification of U2, U4, and U5 snRNAs in the Deep-Branching Eukaryote Entamoeba histolytica

2007 ◽  
Vol 6 (6) ◽  
pp. 940-948 ◽  
Author(s):  
Carrie A. Davis ◽  
Michael P. S. Brown ◽  
Upinder Singh
Keyword(s):  
Entamoeba Histolytica ◽  
Splice Site ◽  
Expressed Sequence Tag ◽  
Functional Characterization ◽  
Molecular Evidence ◽  
Spliceosomal Introns ◽  
Accurate Expression ◽  
Eukaryotic Organisms

ABSTRACT Pre-mRNA splicing is essential to ensure accurate expression of many genes in eukaryotic organisms. In Entamoeba histolytica, a deep-branching eukaryote, approximately 30% of the annotated genes are predicted to contain introns; however, the accuracy of these predictions has not been tested. In this study, we mined an expressed sequence tag (EST) library representing 7% of amoebic genes and found evidence supporting splicing of 60% of the testable intron predictions, the majority of which contain a GUUUGU 5′ splice site and a UAG 3′ splice site. Additionally, we identified several splice site misannotations, evidence for the existence of 30 novel introns in previously annotated genes, and identified novel genes through uncovering their spliced ESTs. Finally, we provided molecular evidence for the E. histolytica U2, U4, and U5 snRNAs. These data lay the foundation for further dissection of the role of RNA processing in E. histolytica gene expression.

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