The Host-Parasite Interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea) II. Electron Microscope Studies of the Tegument

1967 ◽  
Vol 53 (4) ◽  
pp. 703 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Host Parasite ◽  
Cyathocotyle Bushiensis

The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea)

Parasitology ◽  
1968 ◽  
Vol 58 (2) ◽  
pp. 371-375 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Plasma Membranes ◽  
Science Research ◽  
Strong Acid ◽  
Gland Cells ◽  
Adhesive Organ ◽  
Cyathocotyle Bushiensis

A combination of histochemical and electron microscope techniques have demonstrated, in Cyathocotyle bushiensis, alkaline phosphatase activity in the matrix of the tegument, in the distal and basal plasma membranes of the tegument, in the wall of the ducts extending from the adhesive organ gland cells and in the wall of the adhesive organ microvilli. Acid phosphatase activity was much stronger and was present in the tegument matrix and in the granular component of the secretion from the adhesive organ gland cells. Strong acid phosphatase activity was also present in the cisternae of the endoplasmic reticulum of the adhesive organ gland cells.I am greatly indebted to Professor Brough for the excellent facilities available within this department. I also wish to thank Professor J. Sinclair (Department of Mining) for electron microscope facilities extended to me in the early stages of this investigation, and to Mr W. Henderson, Mr T. Davies and Miss M. Williams for their invaluable assistance. The purchase of the Huxley ultramicrotome, coating unit and an AEI EM 6 electron microscope was made possible by a grant from the Science Research Council.

Studies on the host–parasite interface of strigeoid trematodes

Parasitology ◽  
1969 ◽  
Vol 59 (1) ◽  
pp. 245-256 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
The Body ◽  
Placental Function ◽  
Adhesive Organ ◽  
Basement Layer ◽  
Host Parasite ◽  
Surface Specialization ◽  
Research Grant

The adhesive organ of Apatemon gracilis minor Yamaguti, 1933, consists of two lobes lying in a cup-shaped fore-body. The cytoplasmic tegument covering the apposing faces of the lobes is different from that covering their outer surfaces. The covering of the outer surface corresponds to the general tegument present on the rest of the body, whereas that on the inner surfaces is finely pitted and in certain regions is elevated to form a coarse reticulum. This specialized surface is covered externally by a plasma membrane and is in continuity with nucleated cell bodies lying below the basement layer. The cell bodies contain large quantities of granular endoplasmic reticulum as well as several Golgi complexes and numerous mitochondria. Masses of secretion bodies are present and these also occur in the extensions to the external tegument as well as within the external tegument of the lobes. When the parasite is attached the inner faces of the lobes come into contact with the vascular lamina propria of the host. The possible biological role of this specialized host–parasite interface is discussed and it is suggested that this surface specialization may form a morphological basis for the ‘placental’ function suggested for the adhesive organ by earlier workers.The author wishes to acknowledge the research grant provided by the S.R.C. for the purchase of a vacuum coating unit and an AEI EM 6 electron microscope. The progress of this study was greatly facilitated by the excellent assistance of Mr T. Davies and Miss C. Green. The Stereoscan micrographs are published by permission of the Cambridge Instrument Company.

The host-parasite interface of strigeoid trematodes

Parasitology ◽  
1970 ◽  
Vol 61 (1) ◽  
pp. 35-41 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
The Body ◽  
Sense Organs ◽  
Adhesive Organ ◽  
Transmission Electron ◽  
Transmission Studies ◽  
Host Parasite ◽  
Surface Specialization

A detailed study of the structure of the tegument of D. phoxini has been carried out using probe and transmission electron microscope. The distribution of spines and sense organs on the forebody has been described, as well as the nature of the lappets in the everted and retracted state. The adhesive organ exhibits two different surfaces which also differ from the surface of the rest of the body. The use of the ‘Stereoscan’ has confirmed and extended the concept of surface specialization based on transmission studies.

Studies on the host–parasite interface of strigeoid trematodes

Parasitology ◽  
1969 ◽  
Vol 59 (1) ◽  
pp. 193-201 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Granular Material ◽  
Host Tissue ◽  
Gland Cells ◽  
Host Parasite ◽  
Fine Granular Material ◽  
Research Grant

The ultrastructure of the lappets of Apatemon gracilis minor Yamaguti, 1933, has been described. The tegument covering the lappets is characterized by the presence of long seta-like structures and unicellular gland cells which exhibit a holocrine type of secretion. The setae contain extensions of the tegument cytoplasm and are supported by a bundle of fibrils. The unicellular gland cells are pear-shaped with the tapering neck supported by a ring of 50–60 microtubules. The secretion consists of relatively large, membrane bounded bodies containing a fine granular material.The lappets are regarded as representing a specialized host–parasite interface in which adaptation exists for attachment to the host tissue and the discharge of secretion from the parasite at this region. The concept of the specialized host–parasite interface is discussed and extended to include Apatemon gracilis minor.The author wishes to acknowledge the research grant provided by the S.R.C. for the purchase of a vacuum coating unit and an AEI EM 6 electron microscope. The progress of this study was greatly facilitated by the excellent assistance of Mr T. Davies and Miss C. Green. The stereoscan micrograph (P1. 1, fig. 2) is published by permission of the Cambridge Instrument Company.

Fasciola hepatica: development of the tegument of normal and γ-irradiated flukes during infection in rats and mice

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 137-145 ◽  
Author(s):  
D. J. Burden ◽  
A. P. Bland ◽  
D. L. Hughes ◽  
N. C. Hammet
Keyword(s):  
Electron Microscope ◽  
Fasciola Hepatica ◽  
Secretory Granules ◽  
Γ Irradiation ◽  
Variable Effect ◽  
Transmission Electron ◽  
Parasite Survival ◽  
Host Parasite ◽  
Rats And Mice ◽  
Significant Production

SUMMARYRats and mice were infected with either normal metacercariae or metacercariae γ-irradiated at 3 krad. or 4 krad. Flukes were recovered at various times after infection and their teguments examined using a transmission electron microscope. In normal flukes, the secretory granules TO, Tl and T2 were all seen during tegumental development. The teguments of flukes from mice developed faster than the corresponding teguments in rats. TO granules were present from day 0 to day 10 post-infection (p.i.) in mouse flukes and from day 0 to day 14 p.i. in rat flukes. Tl granules first appeared in mouse flukes by day 4 p.i. but not until day 8 p.i. in rat flukes. T2 granules were seen in mouse flukes 2 days p.i. but not before 6 days p.i. in rat flukes. γ-Irradiation at 4 krad prevented normal tegumental development in flukes from both rats and mice. TO granules were present at all times in flukes from either host. Tl granules were produced in mouse flukes but their appearance was delayed until day 6 p.i. No significant production of T2 granules occurred in flukes from either host. Parasite survival was also affected by γ-irradiation and none of the flukes reached maturity. Flukes from rats died between 10 and 21 days p.i and flukes from mice died between 14 and 28 days p.i. γ-Irradiation of metacercariae at 3 krad. had an extremely variable effect on subsequent tegumental development in both rats and mice. Some flukes developed normally, some showed development associated with γ-irradiation at 4 krad, whilst some showed intermediate development.

Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

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