Parasites of Ovis canadensis canadensis in Montana, with a Checklist of the Internal and External Parasites of the Rocky Mountain Bighorn Sheep in North America

1967 ◽  
Vol 53 (1) ◽  
pp. 157 ◽  
Author(s):  
Willard W. Becklund ◽  
Clyde M. Senger
2004 ◽  
Vol 40 (2) ◽  
pp. 311-315 ◽  
Author(s):  
Terry J. Kreeger ◽  
Walter E. Cook ◽  
William H. Edwards ◽  
Todd Cornish

1987 ◽  
Vol 65 (8) ◽  
pp. 2028-2034 ◽  
Author(s):  
Henry J. Harlow ◽  
E. Tom Thorne ◽  
Elizabeth S. Williams ◽  
E. Lee Belden ◽  
William A. Gern

It was the purpose of this study to investigate methods of assessing responses to stress by free-ranging bighorn sheep (Ovis canadensis canadensis). The adrenal response test on wild-caught bighorn sheep maintained in captivity did not demonstrate either adrenal exhaustion or hypersensitivity during chronic stress. To study physiological responses to acute stress, hand-reared bighorn sheep were habituated to living in stalls and fitted with electrocardiogram leads and jugular cannulas for remote monitoring of cardiac frequency and blood cortisol changes. A radioimmunoassay was validated on bighorn sheep plasma which was a modification of the procedure used for domestic sheep. A linear relationship between heart rate and blood cortisol was obtained for individual animals suggesting that remote monitoring of cardiac frequency can be used as a predictor of adrenal function and, therefore, the potential immunologic condition of an animal during stress.


1965 ◽  
Vol 43 (5) ◽  
pp. 885-888 ◽  
Author(s):  
E. C. H. Chen ◽  
D. A. Blood ◽  
B. E. Baker

Milk was obtained from five Rocky Mountain bighorn sheep living in the Jasper Park area of Alberta. The milk was collected at [Formula: see text] and 3 months post partum. The gross composition and milk fat fatty acids of the milk samples and of a sample of domestic sheep milk (Suffolk, 1 month post partum) were determined.


2016 ◽  
Vol 94 (11) ◽  
pp. 733-745 ◽  
Author(s):  
Kim G. Poole ◽  
Rob Serrouya ◽  
Irene E. Teske ◽  
Kevin Podrasky

Winter is an important period for most mountain ungulates due to limited availability of preferred forage and costs associated with travel through deep snow. We examined winter habitat selection by Rocky Mountain bighorn sheep (Ovis canadensis canadensis Shaw, 1804) where four large, open-pit coal mines are in operation. Sheep in this area generally winter at high elevation on windswept, south-facing native grasslands. We used GPS collars and Resource Selection Function analysis to examine movements and habitat selection. A majority (79%) of the sheep were migratory and fidelity to winter ranges was high (88%). Sheep showed low use (∼10%–20%) of mine areas between November and April, followed by increased use peaking at 60%–65% in September–October. Wintering sheep were positively associated with high elevations, closeness to escape terrain, and warmer aspects. High-elevation, native grasslands were the highest ranked cover class. Most sheep that used mine areas during winter used reclaimed habitats, primarily reclaimed spoils and pits. Primary winter ranges comprised 4.3% of merged sheep range, emphasizing the limited amount of occupied winter ranges within the landscape. Disturbance to native winter range resulting from development should be minimized or be conducted in a manner that effectively manages and (or) mitigates the impacts.


2010 ◽  
Vol 48 (3) ◽  
pp. 706-712 ◽  
Author(s):  
K. A. Fox ◽  
S. K. Wootton ◽  
S. L. Quackenbush ◽  
L. L. Wolfe ◽  
I. K. LeVan ◽  
...  

2014 ◽  
Vol 26 (1) ◽  
pp. 133 ◽  
Author(s):  
T. Stroud ◽  
T. Xiang ◽  
S. Romo ◽  
M. E. Kjelland

The present objective was to determine if interspecies somatic cell nuclear transfer (SCNT) could be a viable option for producing cloned Rocky Mountain bighorn sheep (RMBS) embryos, a model for other endangered ovine species or sheep of high genetic value. A primary question was whether viable cells from a harvested 5.5-year-old male RMBS (Ovis canadensis canadensis) could be cultured 4 days postmortem for SCNT embryo production. Domestic sheep ovaries from an abattoir were placed in TCM199 holding medium containing Hanks’ salts, kept at ambient temperature, and transported to the laboratory in less than 3 h. Oocytes were collected using 2 methods, aspirating visible follicles (G1) or slicing ovary tissues (G2), and matured in TCM199-based medium. At 20 h postmaturation, oocytes were stripped of cumulus cells and those with an extruded polar body underwent SCNT using ViaGen's protocol. Tissue samples were collected from the RMBS, placed in plastic bags, and shipped to the laboratory for cell culture; that is, tissue disinfection and mincing, placement in cell culture flasks with cell culture medium and into an incubator at 38.5°C in a humidified atmosphere of 5% CO2. At confluency, cells having fibroblast-like cell morphology were cryopreserved using DMSO-containing medium and stored at –196°C. For SCNT, 4-day postmortem donor cells were thawed and cultured for at least 3 days. Matured oocytes were enucleated using a micromanipulation system and Hoechst 33342 to ensure nucleus removal. A donor cell was inserted into an enucleated cytoplast and fusion pulses were applied to reconstruct embryos. Cloned embryos were cultured in modified SOF in 5% CO2, 5% O2, and 90% N2 for 6 days before shipment in a portable incubator at 38.5°C in SOF for analysis on Day 7. Pearson's χ2 test and Fisher's exact test were used to determine statistical differences (α = 0.05). The 60 ovaries yielded 324 oocytes (5.4 oocytes/ovary) and 217 matured (67%) of which 204 were enucleated, 156 fused (77%), and 102 cleaved (65%), producing 25 blastocysts and morulae (25/156 = 16%). G1 resulted in 62 cleaved embryos of 91 fused, whereas G2 resulted in 40 cleaved embryos of 65 fused (χ2 = 0.728; P = 0.393). Using the IETS stage and grading system, 3 blastocysts (one 5–2, one 6–1, one 6–2) and 22 morulae (4–1) appeared viable, but the rest had degenerated. Specifically, G1 produced 3 blastocysts and 8 stage 4–1 embryos versus 14 stage 4–1 embryos in G2 (based on fused or cleaved embryos: χ2 = 2.516, P = 0.113 and χ2 = 3.914, P = 0.048, respectively). Fibroblast-like cell cultures were obtained from 4- and 5-day postmortem tissue samples; however, only Day 4 cultured cells were used for RMBS SCNT embryo production. Although G1 produced more blastocysts than G2, the difference was not statistically significant (P > 0.05).


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