Sucrose Uptake and Transport in Conducting Cells of Polytrichum commune

1981 ◽  
Vol 84 (1) ◽  
pp. 59 ◽  
Author(s):  
David A. Reinhart ◽  
Robert J. Thomas
Keyword(s):  
Sucrose Uptake ◽  
Polytrichum Commune ◽  
Uptake And Transport

Use of SEM, X-ray analysis and TEM to localize Fe3+ reduction in roots

1988 ◽  
Vol 46 ◽  
pp. 392-393 ◽  
Author(s):  
William P. Wergin ◽  
P. F. Bell ◽  
Rufus L. Chaney
Keyword(s):  
Electron Microscopy ◽  
Root Hairs ◽  
Rapid Reduction ◽  
X Ray ◽  
Physical Evidence ◽  
Transmission Electron ◽  
Young Root ◽  
Insoluble Precipitate ◽  
Uptake And Transport ◽  
Scanning Electron

In dicotyledons, Fe3+ must be reduced to Fe2+ before uptake and transport of this essential macronutrient can occur. Ambler et al demonstrated that reduction along the root could be observed by the formation of a stain, Prussian blue (PB), Fe4 [Fe(CN)6]3 n H2O (where n = 14-16). This stain, which is an insoluble precipitate, forms at the reduction site when the nutrient solution contains Fe3+ and ferricyanide. In 1972, Chaney et al proposed a model which suggested that the Fe3+ reduction site occurred outside the cell membrane; however, no physical evidence to support the model was presented at that time. A more recent study using the PB stain indicates that rapid reduction of Fe3+ occurs in a region of the root containing young root hairs. Furthermore the most pronounced activity occurs in plants that are deficient in Fe. To more precisely localize the site of Fe3+ reduction, scanning electron microscopy (SEM), x-ray analysis, and transmission electron microscopy (TEM) were utilized to examine the distribution of the PB precipitate that was induced to form in roots.

Effect of Green Tea and (-)-Epigallocatechin Gallate on the Pharmacokinetics of Rosuvastatin

2020 ◽  
Vol 21 (6) ◽  
pp. 471-478
Author(s):  
Shenjia Huang ◽  
Qingqing Xu ◽  
Linsheng Liu ◽  
Yicong Bian ◽  
Shichao Zhang ◽  
...  
Keyword(s):  
Green Tea ◽  
Cell Model ◽  
Hek293t Cell ◽  
Epigallocatechin Gallate ◽  
Drug Uptake ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Time Curve ◽  
Uptake And Transport

Background: Green tea can inhibit OATPs, so it may interact with the substrate of OATPs, such as rosuvastatin. Objective: This study aimed to investigate the effects of green tea on the pharmacokinetics of rosuvastatin and its mechanism. Methods: Male Sprague-Dawley rats received different doses of green tea extract (GTE) and (-)- epigallocatechin-3- gallate (EGCG). Caco-2 cells and OATP1B1-HEK293T cells were used in drug uptake and transport assay. The matrix concentrations of rosuvastatin and catechins were determined by ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS). Results: GTE and EGCG were both found to increase the area under the plasma concentration-time curve (AUC0-∞) of rosuvastatin ((p<0.050). In the Caco-2 cell model, the uptake and transport of rosuvastatin in the GTE groups were 1.94-fold (p<0.001) and 2.11-fold (p<0.050) higher, respectively, than those of the control group. However, in the EGCG group, the uptake and transport of rosuvastatin were decreased by 22.62% and 44.19%, respectively (p<0.050). In the OATP1B1- HEK293T cell model, the OATP1B1-mediated rosuvastatin uptake was decreased by GTE to 35.02% of that in the control (p<0.050) and was decreased by EGCG to 45.61% of that in the control (p<0.050). Conclusion: GTE increased the systemic rosuvastatin exposure in rats. The mechanism may include an increase in rosuvastatin absorption and a decrease in liver distribution by inhibiting OATP1B1. EGCG may be the main ingredient of green tea that affects the pharmacokinetic parameters of rosuvastatin. Our results showed the importance of conducting green tea-rosuvastatin study.

Physiological and molecular insights involved in silicon uptake and transport in ryegrass

2021 ◽  
Author(s):  
Sofía Pontigo ◽  
Giovanni Larama ◽  
Leyla Parra-Almuna ◽  
Adriano Nunes-Nesi ◽  
María de la Luz Mora ◽  
...  
Keyword(s):  
Silicon Uptake ◽  
Uptake And Transport

Factors affecting 14C-sucrose uptake by single-node explants of rose flower stems

1980 ◽  
Vol 50 (3) ◽  
pp. 251-254 ◽  
Author(s):  
GERARD JACOBS ◽  
NAFTALY ZIELIN ◽  
ABRAHAM H. HALEVY
Keyword(s):  
Sucrose Uptake ◽  
Single Node ◽  
Factors Affecting ◽  
Rose Flower

AGE-Induced Interference of Glucose Uptake and Transport as a Possible Cause of Insulin Resistance in Adipocytes

2011 ◽  
Vol 59 (14) ◽  
pp. 7978-7984 ◽  
Author(s):  
Chi-Hao Wu ◽  
Hsiao-Wen Huang ◽  
Shang-Ming Huang ◽  
Jer-An Lin ◽  
Chi-Tai Yeh ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Glucose Uptake ◽  
Possible Cause ◽  
Uptake And Transport

O1-07-03: Aggregation and size-dependent uptake and transport of tau aggregates in neurons

2012 ◽  
Vol 8 (4S_Part_3) ◽  
pp. P97-P97
Author(s):  
Jessica Wu ◽  
Mathieu Herman ◽  
Karen Duff
Keyword(s):  
Size Dependent ◽  
Uptake And Transport
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