Time-Lapse Light Photomicrography and Scanning Electron Microscopy of Somatic Embryo Ontogeny from Cultured Leaves of Dactylis glomerata (Gramineae)

1985 ◽  
Vol 104 (4) ◽  
pp. 395 ◽  
Author(s):  
D. J. Gray ◽  
B. V. Conger
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Embryo ◽  
Dactylis Glomerata ◽  
Time Lapse ◽  
Scanning Electron

Panicle, spikelet, and floret development in orchardgrass (Dactylis glomerata)

1993 ◽  
Vol 71 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Joanna Fraser ◽  
Eric G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Unique Feature ◽  
Dactylis Glomerata ◽  
Bilateral Symmetry ◽  
Second Order ◽  
Third Order ◽  
First Order ◽  
Lateral Branches ◽  
Scanning Electron

The initial stages of panicle, spikelet, and floret development in field-grown 'Kay' orchardgrass were examined using scanning electron microscopy. Spikelets arose from a complex multilevelled sequence of initiation from branch apices. Spikelets developed indirectly in a two-tiered progression: (i) an acropetal and basipetal sequence of first order, second-order, and third-order inflorescence apices, and (ii) an acropetal development within subclusters of higher-order lateral branch inflorescence apices. The panicle had the unique feature of dorsiventrality as well as bilateral symmetry. The basal apex from first-order, second-order, or third-order apices developed on the same side of the main axis as the first-order apex. The two glumes subtending each spikelet primordium developed alternately and acropetally. Development and initiation of florets within spikelets was basipetal within the panicle, basipetal within clusters and subclusters of spikelets on lateral branches, and acropetal within spikelets. Within florets, paleas developed later than lemmas. Key words: Dactylis glomerata, cocksfoot, scanning electron microscopy, development, panicle.

A different type of amphibian mesoderm morphogenesis in Ceratophrys ornata

Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 307-317 ◽  
Author(s):  
S.M. Purcell ◽  
R. Keller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Layer ◽  
Xenopus Laevis ◽  
Dorsal Surface ◽  
Time Lapse ◽  
Surface Epithelium ◽  
Histological Sections ◽  
Fourth Zone ◽  
Scanning Electron

Ceratophrys ornata, the Argentinean horned frog, has a significantly different pattern of early morphogenesis than does the most studied amphibian, Xenopus laevis. Time-lapse videomicroscopy, scanning electron microscopy, histological sections and lineage tracers have shown that, in C. ornata, some prospective notochord, somite and tailbud mesoderm cells leave the surface epithelium of the archenteron by ingression. After gastrulation, SEM reveals cells with constricted apices and a bottle shape in three zones on the archenteron roof and in a fourth zone around the blastopore. Prospective somitic tissue ingresses first from two lateral zones, followed by ingression of prospective notochord from the medial zone and tailbud mesoderm from the circumblastoporal zone. This is unlike X. laevis, in which no cells with constricted apices are present on the dorsal surface of the archenteron, nor do any cells ingress into the deep mesodermal layers from the surface layer.

Fasciola hepatica: surfaces involved in movement of miracidia and cercariae

2001 ◽  
Vol 75 (1) ◽  
pp. 1-5 ◽  
Author(s):  
C.E. Bennett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fasciola Hepatica ◽  
Time Lapse ◽  
Ventral Surface ◽  
The Body ◽  
Power Stroke ◽  
Free Swimming ◽  
Lateral Plane ◽  
Scanning Electron

Rapid freezing and substitution with fixative prior to scanning electron microscopy was used to demonstrate the pattern of beat and recovery of the cilia of free swimming miracidia ofFasciola hepatica. There were stages of dexioplectic metachronal co-ordination and the power stroke was approximately 15° anticlockwise from the anterior–posterior axis. Around the circumference of the body of the miracidia there were approximately 12 metachronal waves of power and recovery. Free-swimming cercariae were recorded by time-lapse photography and, after conventional fixation, by scanning electron microscopy. Cercarial tail-beats were to the posterior of the body in the lateral plane at a rate of 8 Hz. The tail has paired lateral ridges positioned to act as leading edges. There is an array of 32 sensory papillae on the mid-ventral surface of the tail. The tegument of the most distal part of the tail is described: it is free of sensory endings and the surface shows a spiral pattern.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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