Observations on Staining the Flagella on Motile Bacteria

1891 ◽  
Vol 13 ◽  
pp. 85
Author(s):  
Veranus A. Moore
Keyword(s):  
2021 ◽  
Vol 15 (3) ◽  
pp. 034108
Author(s):  
Jyoti P. Gurung ◽  
Moein Navvab Kashani ◽  
Sanaz Agarwal ◽  
Gonzalo Peralta ◽  
Murat Gel ◽  
...  

2021 ◽  
Vol 103 (1) ◽  
Author(s):  
Lazaro J. Perez ◽  
Tapomoy Bhattacharjee ◽  
Sujit S. Datta ◽  
Rishi Parashar ◽  
Nicole L. Sund

2007 ◽  
Vol 189 (23) ◽  
pp. 8704-8707 ◽  
Author(s):  
Peter Galajda ◽  
Juan Keymer ◽  
Paul Chaikin ◽  
Robert Austin

ABSTRACT Randomly moving but self-propelled agents, such as Escherichia coli bacteria, are expected to fill a volume homogeneously. However, we show that when a population of bacteria is exposed to a microfabricated wall of funnel-shaped openings, the random motion of bacteria through the openings is rectified by tracking (trapping) of the swimming bacteria along the funnel wall. This leads to a buildup of the concentration of swimming cells on the narrow opening side of the funnel wall but no concentration of nonswimming cells. Similarly, we show that a series of such funnel walls functions as a multistage pump and can increase the concentration of motile bacteria exponentially with the number of walls. The funnel wall can be arranged along arbitrary shapes and cause the bacteria to form well-defined patterns. The funnel effect may also have implications on the transport and distribution of motile microorganisms in irregular confined environments, such as porous media, wet soil, or biological tissue, or act as a selection pressure in evolution experiments.


1928 ◽  
Vol 48 (6) ◽  
pp. 825-836
Author(s):  
John B. Nelson

It was shown that flocculating (flagellar) agglutinin and granulating (somatic) agglutinin display certain differences with respect to their removal from sensitized bacteria (B. paratyphi). A 5 per cent solution of NaCl added to sedimented, sensitized bacteria followed by heating to 60°C. for 1 hour removed approximately 50 per cent of the combined agglutinin. There was little or no removal of granulating agglutinin either from the sensitized motile bacteria or from a sensitized non-motile organism (Staphylococcus). Evidence was presented that the agglutinin removal was not dependent solely on disintegration of flagella by the conditions of extraction with a subsequent freeing of antibody.


2019 ◽  
Vol 3 (2) ◽  

Sperm immobilization factor (SIF), the secretory protein of Staphylococcus aureus, is known to cause complete immobilization, death and morphological alterations in mouse spermatozoa in vitro. However, the present study aims to explore a newer dimension of SIF i.e., to bind to motile and non-motile bacteria and its ability to induce immobilization of motile bacteria in vitro. The results showed that 800µg of SIF caused complete immobilization of motile bacteria, however, death and morphological alterations could not be observed even with 1000µg of SIF. Furthermore, this SIF-mediated bacterial immobilization was reversed when each of the SIF-binding receptor from mouse spermatozoa and bacteria (Escherichia coli and Streptococcus pyogenes) was incubated with bacteria, thereby, providing an experimental evidence of similarity between the antigenic determinants present on spermatozoa and bacteria against a common ligand, SIF.


Lab on a Chip ◽  
2014 ◽  
Vol 14 (5) ◽  
pp. 1023-1032 ◽  
Author(s):  
Takuji Ishikawa ◽  
Tatsuya Shioiri ◽  
Keiko Numayama-Tsuruta ◽  
Hironori Ueno ◽  
Yohsuke Imai ◽  
...  

Separation of certain bacteria from liquids is important in the food, water quality management, bioengineering, and pharmaceutical industries.


2017 ◽  
Vol 194 ◽  
pp. 58-66 ◽  
Author(s):  
Ramona Bosse ◽  
Nadine Thiermann ◽  
Monika Gibis ◽  
Herbert Schmidt ◽  
Jochen Weiss

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Stanislaw Makarchuk ◽  
Vasco C. Braz ◽  
Nuno A. M. Araújo ◽  
Lena Ciric ◽  
Giorgio Volpe

Abstract How motile bacteria move near a surface is a problem of fundamental biophysical interest and is key to the emergence of several phenomena of biological, ecological and medical relevance, including biofilm formation. Solid boundaries can strongly influence a cell’s propulsion mechanism, thus leading many flagellated bacteria to describe long circular trajectories stably entrapped by the surface. Experimental studies on near-surface bacterial motility have, however, neglected the fact that real environments have typical microstructures varying on the scale of the cells’ motion. Here, we show that micro-obstacles influence the propagation of peritrichously flagellated bacteria on a flat surface in a non-monotonic way. Instead of hindering it, an optimal, relatively low obstacle density can significantly enhance cells’ propagation on surfaces due to individual forward-scattering events. This finding provides insight on the emerging dynamics of chiral active matter in complex environments and inspires possible routes to control microbial ecology in natural habitats.


1961 ◽  
Vol 25 (2) ◽  
pp. 253-290 ◽  
Author(s):  
E. W. MEYNELL
Keyword(s):  

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