The Intensity of Maize Processing and Production in Upland Mogollon Pithouse Villages A.D. 200–1000

1996 ◽  
Vol 61 (1) ◽  
pp. 102-115 ◽  
Author(s):  
Michael W. Diehl

Analyses of the size, shape, and wear on western Mogollon manos and metates reveal that the dietary importance of maize remained low and stable from the Early Pithouse period (A.D. 200–550) through the Georgetown phase (A.D. 550–700). The consumption of maize increased during the San Francisco phase (A.D. 700–825/850) and continued to increase through the Three Circle phase (A.D. 825/850–1000). Changes in the ubiquity of charred pieces of maize (Zea mays) from paleoethnobotanical samples also indicate an increase in maize consumption from the Early Pithouse period through the Three Circle phase. The onset of increased maize consumption roughly coincided with the introduction of an improved variety of eight-row maize, around A.D. 650–700 (Upham et al. 1987). The analyses presented in this study do not agree with recent suggestions (Gilman 1987; Mauldin 1991) that maize consumption in the western Mogollon region remained stable and low until the Classic Mimbres phase (A.D. 1000–1150).

1956 ◽  
Vol 21 (4) ◽  
pp. 412-412
Author(s):  
Jack T. Hughes

Several Southwestern archaeologists who have seen the mat pictured in Figure 136 consider it unusual enough to merit description in print, despite a scarcity of information about its provenience and associations.The mat has catalog number 943/7 in the collections of the Panhandle-Plains Historical Museum at Canyon, Texas. It was donated to the Museum by Henry Crain of Canyon in 1942. Mr. Crain informs me that he found the mat in a multiroomed cave about 1/2 mile above the mouth of Mule Creek, a tributary of the San Francisco River in the Mogollon Range of southwestern New Mexico, not far from Clifton, Arizona. He and several hunting companions removed the mat and other archaeological materials from the cave in 1899, when he was ranching in the Mogollon region. Other items included 2 small brown pottery bowls, 2 wooden bows 4 or 5 feet long, and a number of obsidian-tipped arrows.


Author(s):  
O. E. Bradfute ◽  
R. E. Whitmoyer ◽  
L. R. Nault

A pathogen transmitted by the eriophyid mite, Aceria tulipae, infects a number of Gramineae producing symptoms similar to wheat spot mosaic virus (1). An electron microscope study of leaf ultrastructure from systemically infected Zea mays, Hordeum vulgare, and Triticum aestivum showed the presence of ovoid, double membrane bodies (0.1 - 0.2 microns) in the cytoplasm of parenchyma, phloem and epidermis cells (Fig. 1 ).


Author(s):  
O. E. Bradfute

Maize mosaic virus (MMV) causes a severe disease of Zea mays in many tropical and subtropical regions of the world, including the southern U.S. (1-3). Fig. 1 shows internal cross striations of helical nucleoprotein and bounding membrane with surface projections typical of many plant rhabdovirus particles including MMV (3). Immunoelectron microscopy (IEM) was investigated as a method for identifying MMV. Antiserum to MMV was supplied by Ramon Lastra (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela).


Author(s):  
O. E. Bradfute

Maize rayado fino virus (MRFV) causes a severe disease of corn (Zea mays) in many locations throughout the neotropics and as far north as southern U.S. MRFV particles detected by direct electron microscopy of negatively stained sap from infected leaves are not necessarily distinguishable from many other small isometric viruses infecting plants (Fig. 1).Immunosorbent trapping of virus particles on antibody-coated grids and the antibody coating or decoration of trapped virus particles, was used to confirm the identification of MRFV. Antiserum to MRFV was supplied by R. Gamez (Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica).Virus particles, appearing as a continuous lawn, were trapped on grids coated with MRFV antiserum (Fig. 2-4). In contrast, virus particles were infrequently found on grids not exposed to antiserum or grids coated with normal rabbit serum (similar to Fig. 1). In Fig. 3, the appearance of the virus particles (isometric morphology, 30 nm diameter, stain penetration of some particles, and morphological subunits in other particles) is characteristic of negatively stained MRFV particles. Decoration or coating of these particles with MRFV antiserum confirms their identification as MRFV (Fig. 4).


Author(s):  
László G. Kömüves

Light microscopic immunohistochemistry based on the principle of capillary action staining is a widely used method to localize antigens. Capillary action immunostaining, however, has not been tested or applied to detect antigens at the ultrastructural level. The aim of this work was to establish a capillary action staining method for localization of intracellular antigens, using colloidal gold probes.Post-embedding capillary action immunocytochemistry was used to detect maternal IgG in the small intestine of newborn suckling piglets. Pieces of the jejunum of newborn piglets suckled for 12 h were fixed and embedded into LR White resin. Sections on nickel grids were secured on a capillary action glass slide (100 μm wide capillary gap, Bio-Tek Solutions, Santa Barbara CA, distributed by CMS, Houston, TX) by double sided adhesive tape. Immunolabeling was performed by applying reagents over the grids using capillary action and removing reagents by blotting on filter paper. Reagents for capillary action staining were from Biomeda (Foster City, CA). The following steps were performed: 1) wet the surface of the sections with automation buffer twice, 5 min each; 2) block non-specific binding sites with tissue conditioner, 10 min; 3) apply first antibody (affinity-purified rabbit anti-porcine IgG, Sigma Chem. Co., St. Louis, MO), diluted in probe diluent, 1 hour; 4) wash with automation buffer three times, 5 min each; 5) apply gold probe (goat anti-rabbit IgG conjugated to 10 nm colloidal gold, Zymed Laboratories, South San Francisco, CA) diluted in probe diluent, 30 min; 6) wash with automation buffer three times, 5 min each; 7) post-fix with 5% glutaraldehyde in PBS for 10 min; 8) wash with PBS twice, 5 min each; 9) contrast with 1% OSO4 in PBS for 15 min; 10) wash with PBS followed by distilled water for5 min each; 11) stain with 2% uranyl acetate for 10 min; 12) stain with lead citrate for 2 min; 13) wash with distilled water three times, 1 min each. The glass slides were separated, and the grids were air-dried, then removed from the adhesive tape. The following controls were used to ensure the specificity of labeling: i) omission of the first antibody; ii) normal rabbit IgG in lieu of first antibody; iii) rabbit anti-porcine IgG absorbed with porcine IgG.


2011 ◽  
Vol 20 (1) ◽  
pp. 17-18 ◽  
Author(s):  
Lateef McLeod

Abstract Individuals with significant communication challenges need to communicate across many different venues. The author, from the perspective of an individual who uses AAC, discusses the strengths and weaknesses of both traditional AAC technologies and new mobile AAC technologies. He describes how access to AAC has allowed him to fulfill his dreams as a presenter and writer. He successfully manages a blog in San Francisco, writes grants, and has published his first book of poetry. Not one AAC device fits all of his communication needs; however, access to mobile technology tools has increased his flexibility across environments and given him another successful tool for communication.


2005 ◽  
Vol 173 (4S) ◽  
pp. 34-34
Author(s):  
Viraj A. Master ◽  
Jennifer Young ◽  
Jack W. McAninch

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