Testing Flotation Recovery Rates

1982 ◽  
Vol 47 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Gail E. Wagner

The poppy seed recovery test is a simple and inexpensive means of determining the effectiveness and consistency of any particular botanical flotation system. By adding a known number of charred poppy seeds (Papaver somniferum) to a sample before it is processed, archaeologists can check for loss, damage, and inter-sample contamination. Different systems, equipment, methods, and personnel tested since 1976 yield recovery rate percentages ranging from 6% to 98%. Such percentages provide a basis of comparison among widely differing flotation systems and enable the archaeologist to decide which flotation system to use under particular logistical constraints.

2011 ◽  
Vol 52 (No. 6) ◽  
pp. 282-288 ◽  
Author(s):  
J. Lachman ◽  
A. Hejtmánková ◽  
D. Miholová ◽  
D. Kolihová ◽  
P. Tluka

The effort of this work was to prove whether there exists any relation between stress factors caused by content of cadmium and zinc, and content and composition of opium alkaloids codeine, morphine, narcotine (noscapine) and papaverine contained in poppy seeds and capsules (Papaver somniferumL.) in 14 samples of 8 varieties cultivated in 8 localities in 2003. Contents of cadmium and zinc were measured by a method of electrothermic atomization and contents of selected individual alkaloids were determined by a HPLC method. The results were tested by the Spearman correlation coefficient and the Pearson linear correlation coefficient. With knowledge of the Spearman coefficient, positive relations narcotine – zinc and narcotine – cadmium in the poppy seed samples were found. In the samples of poppy capsules positive relation morphine – cadmium (in cv. Opal) and negative relation papaverine – zinc and codeine – zinc (in cv. Opal) were found. Those pairs have been tested by the Pearson correlation coefficient for a possibility to exclude the linear independency. This independency was excluded with the probability of over 95% in the relations narcotine – cadmium in the seeds and morphine – cadmium in the capsules. With the probability of over 97.5%, linear relations narcotine – zinc in seeds and papaverine – zinc in capsules were proved.


2017 ◽  
Vol 9 (1) ◽  
pp. 114-120
Author(s):  
Saniya B. Kamal ◽  
Arvind Kumar ◽  
Tanuj Tanwar

Papaver somniferum(Poppy) seeds contain poly-phenols like tannic acid, ellagitannin that act as antioxidant, fat replacer, sedative, analgesic and anti-tussive properties, disease preventing and health promoting proper-ties. Efficacy of ground P. somniferum poppy seed was used in chevon nuggets was analyzed. The use of ground poppy seed in chevon nuggets formulation had no effect on moisture as well as pH content of poppy seed incorporated chevon nuggets. However, ground poppy seed incorporation in chevon nuggets had significantly (p<0.05) lower fat content, higher protein content, higher emulsion stability and subsequently higher cooking yield. Chevon nuggets having 10% ground poppy seed were having significantly (p<0.05) higher sensory scores. The ground poppy seed treated chevon nuggets were having significantly (p<0.05) lower TBA and FFA value. The microbial load of developed product was significantly (p<0.05) lower during refrigeration storage. The chevon nuggets prepared with fortification of ground poppy seed was found to be suitable for consumption till 21st day during refrigeration storage based on TBA, FFA, microbiological and sensory profile. Thus, chevon nuggets with good to very good acceptability were developed with incorporation of ground poppy (P. somniferum) seed in it. The chevon nuggets developed consisted of higher amount of antioxidants and polyunsaturated fatty acid with better sensory scores and longer shelf-life.


1992 ◽  
Vol 32 (4) ◽  
pp. 296-302 ◽  
Author(s):  
D S T Lo ◽  
T H Chua

Three white poppy seed samples of Papaver somniferum L were found to contain total morphine (free and bound) in the range 58.4 to 62.2μg/g seeds and total codeine (free and bound) in the range 28.4 to 54.1μg/g seeds. Soaking seeds in water was found to remove 45.6 per cent of the free morphine and 48.4 per cent of the free codeine. In ingesting a curry meal or two containing various amounts of washed seeds (morphine intake: 200.4 to 1002μg; codeine intake: 95.9 to 479.5μg), the urinary morphine levels were found to be in the range 0.12 to 1.27μg/ml urine and urinary codeine levels in the range 0.04 to 0.73μg/ml urine. In any large scale screening for abuse of opiate drugs, the possibility of urinary alkaloids arising from consuming food containing poppy seeds must be considered and, if possible, eliminated.


1965 ◽  
Vol 52 (18) ◽  
pp. 516-517 ◽  
Author(s):  
Ajit Singh Bhown ◽  
Dinesh K. Shah ◽  
Som Nath

2018 ◽  
Vol 23 (9) ◽  
pp. 1153-1158
Author(s):  
Scott H Waltman

The Improving Access to Psychological Therapies initiative has a reported 50 percent recovery rate. Recently, Scott published a scathing report asserting that 9.2 percent is the correct rate. This alarming assertion requires a closer look and further scrutiny of the Improving Access to Psychological Therapies. Scott’s study is fundamentally flawed in many ways; chiefly, the small ( n = 87), forensically involved, convenience sample is not representative of the Improving Access to Psychological Therapies population. Scott’s study is more a limited-scope non-representative-sampled audit (or pilot study audit) of the Improving Access to Psychological Therapies. Given the large amount of public funds involved in the project, further research and a high degree of transparency is warranted.


2004 ◽  
Vol 16 (2) ◽  
pp. 207 ◽  
Author(s):  
A. Castro Neto ◽  
B.V. Sanches ◽  
S.H.V. Perri ◽  
M.M. Seneda ◽  
J.F. Garcia

The aim of the present study was to evaluate the effects of uterine double flushing on embryo recovery rates (total structures and viable embryos), after a resting period with the uterus filled with PBS. There were 210 embryo recovery procedures conducted using the uterine double flushing method, and the results were compaired with 432 conventional single flushing procedures. All procedures were conducted with Limousin (n=403) and Guzerá (n=239) cows, following the same superovulation protocol. Cyclic donors received a progestagen implant (CIDR) and 1mL of oestradiol benzoate (Estrogin®) at Day 0. Between Day 5 and Day 9 animals received doses ranging from 200 to 300UI (zebu cows) and 300 to 500UI (taurine cows) of FSH (Pluset® – Serono) in decreasing doses. Between Day 6 and Day 8 PGF2a (Ciosin®) was administered, followed by withdrawal of the progestagen after 24h. Artificial insemination was performed between 14 and 26h after the beginning of treatment. For the double flushing procedure, after the first uterine flushing of both horns with 1L DPBS (Nutricell), a Foley catheter was positioned in the uterine body in order to fill the uterus with the same solution (80 to 150mL). After this procedure the catheter was closed with a disposable 5-mL syringe gasket, and the animals were allowed to rest in the surroundings of the work place for 30min. After this period, a second flush was performed in order to recover the remaning liquid used during manipulation of the uterus. Animals from the control group (group A) were subjected to a single uterine flushing procedure. From 210 double flushing procedures (group B – test), 1409 viable embryos were produced, classified as grades I, II, III and IV (IETS), (average of 6.7 embryos per procedure), whereas, in the 432 single flushing procedure (group A – control), 1993 embryos were produced (average of 4.6). Statistical analysis showed the increase of viable embryo recovery rate. When consecutive double flushing was performed, the average of recovered embryos increased from 8.3 to 12.7 (P&lt;0.05) in Limousin cows, and from 7.9 to 11.5 (P&lt;0.05) in Guzerá cows. Comparing recovery after single flushing with that after double flushing, the mean number of viable embryos increased from 4.7 to 6.9 (P&lt;0.05) in Limousin cows and from 4.5 to 6.4 (P&lt;0.05) in Guzerá cows. In order to assure the nonexistence of negligence effects or operator influence on results, the mean values of total embryo recovery rate after single flushing (control group) was compared to the mean values of the same rate after the first uterine flushing on test group. Results indicated no difference between recovery rates. The present work showed the viability of using the uterine double flushing procedure for improvement of embryo recovery rates in cattle.


2013 ◽  
Vol 25 (1) ◽  
pp. 227
Author(s):  
F. Papa ◽  
M. Carmo ◽  
P. Papa ◽  
J. Dell'Aqua ◽  
M. Alvarenga

The aim of this study was to improve the spermatic kinetic parameters from stallions with poor quality of fresh and chilled semen by the use of density gradient Equipure™ (Nidacon, Mölndal, Sweden). Semen from 5 Quarter Horse stallions aged 8 and 16 years with history of low embryo recovery rates were used. The kinetics sperm evaluation was performed by computerized semen analysis (CASA) and plasma membrane integrity with fluorescent probes. The average motility parameters for fresh semen before selection were total motility (MT) 60%, progressive motility (PM) 30%, and plasma membrane integrity (IMP) 60% and for cooled semen (24 h at 5°C) were: MT 50%, MP 18%, and 50% IMP. For the group of fresh and cooled semen with no density gradient selection (NS), mares were inseminated with 1 billion viable sperm diluted in skim milk extender in the uterine body, 24 h after ovulation induction with 1 mg of deslorelin. For EquipureTM selection group (SE) semen was concentrated through Spermfilter membrane™ and resuspended in 5 mL of BotuSemenTM (BotupharmaTM, Brazil). In a 15 mL conic tube 5 mL of EquipureTM was added and another part containing 5 mL of the resuspended sperm was slowly added over the EquipureTM column. The 15 mL conic tube was centrifuged at 400g for 20 min. After centrifugation, the sperm pellet was carefully aspirated and to the pellet was resuspended in 4 mL of BotucrioTM. The sperm recovery rate with EquipureTM was 40%. Deep uterine AI was performed 24 h after ovulation induction with 1 mg of deslorelin. Data were analyzed by ANOVA followed by Tukey’s test (P < 0.05). The analysis of semen after EquipureTM selection resulted in average: 75, 35, 65, and 40%, respectively, for MT, MP, and IMP index and sperm recovery. The embryo recovery rate from the 5 stallions showed the following results: Stallion 1 (fresh semen): 12 mares (NS)/4 embryos (33%) and for group SE 22 mares/16 embryos, (72%; P ≤ 0.05). Stallion 2 (fresh semen): 9 mares (NS)/4 embryos, (44%) and for group SE, 12 mares/8 embryos (66%; P > 0.1). Stallion 3 (fresh semen): 4 mares (NS)/1 embryo (25%) and 7 mares for SE/4 embryos (57%; P > 0.1). Stallion 4 (chilled semen): 4 mares (NS)/0 embryo 0% and for SE group 8 mares/6 embryos 75%; P ≤ 0.001). Stallion 5 (chilled semen): 10 mares (NS)/2 embryos (20%) and for SE 6 mares/3 embryos (50%; P > 0.1). For the overall results, 39 inseminations were performed on the no selected group and 11 embryos recovered (28%) for the selected group 55 inseminations and 37 embryos recovered (67%; P < 0.01). The results clearly showed that selection by sperm density gradient EquipureTM was a very effective technique that allowed an improvement on semen quality and fertility. The authors acknowledge support from FAPESP and Botupharma.


2017 ◽  
Vol 63 (2) ◽  
pp. 62-65 ◽  
Author(s):  
Mircea Dumitru Croitoru ◽  
Ibolya Fülöp ◽  
Maria-Raluca Irimia-Constantin ◽  
Erzsébet Varga ◽  
Hajnal Kelemen ◽  
...  

Abstract Objective: the number of alkaloids like morphine and codeine found in poppy seeds used in food industry are monitored by a directive given by European Food Safety Authority. Based on this regulation the aim of the study was to determine the quantity of morphine and codeine from several brands of poppy seeds. Methods: an HPLC-UV method (205 nm) was developed to measure the quantity of morphine and codeine. Sample preparation was made using recipes posted on Drugs Forum by some users. Limits of detection were not determined because the lowest concentration from the reference (0.1 μg/ml) detected morphine concentrations that are far lower than a limit of toxicological concern. Results: The concentrations, which were found, ranged between Below the Level of Toxicological Concern (BLTC) - 243.26 mg/kg for morphine and BLTC - 88.58 mg/kg for codeine using several methods of preparation. Conclusions: one can observe that there are some brands of poppy seeds which do not respect the regulation about the amount of morphine and codeine. The high amount of morphine in some samples suggests that there are different varieties of poppy seeds, which can be used for an illicit purpose and can lead to addiction or even overdose in some cases.


2012 ◽  
Vol 17 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Masaya Nakamura ◽  
Kanehiro Fujiyoshi ◽  
Osahiko Tsuji ◽  
Tsunehiko Konomi ◽  
Naobumi Hosogane ◽  
...  

Object This study was conducted to determine whether postoperative changes in the fractional anisotropy (FA) value and diffusion tensor imaging of the cervical spinal cord can predict functional outcome for patients with cervical compressive myelopathy (CCM). Methods Twenty patients with CCM were treated by laminoplasty from 2008 to 2009. Both T2-weighted MRI and diffusion tensor imaging were performed before and after surgery. The FA values were analyzed and fiber tracking was performed. The fiber tract (FT) ratio was calculated according to the following formula: (number of fibers at the compressed level)/(number of fibers at the C-2 level) × 100%. The Japanese Orthopaedic Association scoring system for cervical myelopathy was used to determine pre- and postoperative neurological status of the patients, and the Hirabayashi method was used to calculate the recovery rate. Results There was no significant difference in recovery rates between patients with and those without intramedullary high signal intensity on preoperative T2-weighted images. Substantial differences in FA value among spinal cord, bone, and CSF made it difficult to obtain a precise FA value for the compressed spinal cord. There was a significant correlation between the preoperative FT ratio and the recovery rate (p = 0.0006). A poor outcome (recovery rate < 40%) could be anticipated for CCM patients with preoperative FT ratios below 60%. Conclusions The preoperative FT ratio correlated significantly with the recovery rates in CCM patients. Preoperative diffusion tensor tractography can be a new prognostic predictor for neurological recovery in CCM patients after laminoplasty.


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