scholarly journals Localization of plastid DNA replication on a nucleoid structure

1997 ◽  
Vol 84 (8) ◽  
pp. 1028-1041 ◽  
Author(s):  
Andrea M. Nerozzi ◽  
Annette W. Coleman
2002 ◽  
Vol 32 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Stefan K. Mühlbauer ◽  
Andreas Lössl ◽  
Lilia Tzekova ◽  
Zhurong Zou ◽  

1999 ◽  
Vol 181 (18) ◽  
pp. 5783-5789 ◽  
Author(s):  
M. L. Pato ◽  
M. Banerjee

ABSTRACT The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108 allowed efficient Mu replication and was shown to contain a strong gyrase site. However, neither the strong gyrase site from the plasmid pSC101 nor the major gyrase site from pBR322 could promote efficient Mu replication, even though the pSC101 site is a stronger gyrase site than the Mu SGS as assayed by cleavage in the presence of gyrase and the quinolone enoxacin. To look for SGS-like sites in the Escherichia coli chromosome which might be involved in organizing nucleoid structure, fragments of E. coli chromosomal DNA were substituted for the SGS: first, repeat sequences associated with gyrase binding (bacterial interspersed mosaic elements), and, second, random fragments of the entire chromosome. No fragments were found that could replace the SGS in promoting efficient Mu replication. These results demonstrate that the gyrase sites from the transposing phages possess unusual properties and emphasize the need to determine the basis of these properties.


2010 ◽  
Vol 106 (5) ◽  
pp. 1257-1262 ◽  
Author(s):  
Qing Zhao ◽  
Ming Zhang ◽  
Lingxian Hong ◽  
Kefu Zhou ◽  
Yuguang Lin

FEBS Letters ◽  
1975 ◽  
Vol 56 (2) ◽  
pp. 222-225 ◽  
Author(s):  
John Bennett ◽  
Catherine Radcliffe

Author(s):  
Dhruba K. Chattoraj ◽  
Ross B. Inman

Electron microscopy of replicating intermediates has been quite useful in understanding the mechanism of DNA replication in DNA molecules of bacteriophage, mitochondria and plasmids. The use of partial denaturation mapping has made the tool more powerful by providing a frame of reference by which the position of the replicating forks in bacteriophage DNA can be determined on the circular replicating molecules. This provided an easy means to find the origin and direction of replication in λ and P2 phage DNA molecules. DNA of temperate E. coli phage 186 was found to have an unique denaturation map and encouraged us to look into its mode of replication.


2010 ◽  
Vol 34 (8) ◽  
pp. S60-S60
Author(s):  
Yuning Sun ◽  
Fang Li ◽  
Jianming Qiu ◽  
Xiaohong Lu

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