Requirements of Two Temperature-Sensitive Mutant Strains of Neurospora crassa for Growth at Elevated Temperatures

1967 ◽  
Vol 54 (5) ◽  
pp. 582
Author(s):  
Ruth Rogers Wainwright ◽  
Ilda McVeigh
Keyword(s):  
Neurospora Crassa ◽  
Elevated Temperatures ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Mutant Strains ◽  
Two Temperature

scholarly journals Refractile Granular Inclusions in a Temperature-sensitive Mutant of Escherichia coli K-12

1974 ◽  
Vol 27 (5) ◽  
pp. 575
Author(s):  
D JMorton ◽  
AF Egan
Keyword(s):  
Elevated Temperatures ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Subunit Structure ◽  
Sensitive Mutant ◽  
Intact Cells ◽  
Temperature Sensitive Mutant ◽  
Ultrastructural Studies ◽  
Wide Range ◽  
K 12

At the restrictive temperature, cells of a temperature-sensitive mutant of E. coli K-12 lyse, and the culture medium is seen to contain ghosts of cells containing large inclusions that are refractile when viewed by phase-contrast microscopy. The addition of sodium chloride (l %) or sucrose (12, 5 %) protects the cells from lysis and under these conditions the refractile inclusions are seen in the intact cells, which are short filaments. At elevated temperatures cells become sensitive to a wide range of inhibitors. Isolated inclusions consist almost entirely of protein and are digested by Pronase or trypsin. Electrophoresis of inclusions on sodium dodecyl sulphate-polyacrylamide gels shows them to contain a number of protein components. Ultrastructural studies show that granules are commonly found close to the internal surface of the cytoplasmic membrane. In section they are seen to be grainy in appearance but have no ordered structure. Dissociation of the granules by sodium deoxy-cholate or trypsin leads to the formation of spirally wound filaments with a subunit structure.

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