Comparative Assessment of Modes of Reproductive Isolation Among Four Species of Leopard Frogs (Rana pipiens Complex)

Evolution ◽  
1983 ◽  
Vol 37 (1) ◽  
pp. 66 ◽  
Author(s):  
John S. Frost ◽  
James E. Platz
Keyword(s):  
Reproductive Isolation ◽  
Rana Pipiens ◽  
Comparative Assessment ◽  
Leopard Frogs

scholarly journals INDUCTION OF METASTASIS OF FROG CARCINOMA BY INCREASE OF ENVIRONMENTAL TEMPERATURE

1949 ◽  
Vol 89 (3) ◽  
pp. 269-278 ◽  
Author(s):  
Balduin Lucké ◽  
Hans Schlumberger
Keyword(s):  
Tissue Culture ◽  
Elevated Temperature ◽  
Constant Temperature ◽  
Rana Pipiens ◽  
Environmental Temperature ◽  
Kidney Tumors ◽  
Primary Tumors ◽  
Metastatic Dissemination ◽  
Leopard Frogs ◽  
Tumor Bearing

Metastasis of the kidney carcinoma of leopard frogs (Rana pipiens) has been induced by exposing tumor-bearing animals for approximately 50 days to a constant temperature of 28°C. Under these conditions 54 per cent of the frogs developed secondary growths, whereas in groups kept at 18° or at 7° metastatic dissemination was found in only 6 per cent. Moreover, at the elevated temperature the metastases were usually more numerous and more widely disseminated; they were also fairly uniform in size, suggesting that they had developed at nearly the same time. Dissemination of the kidney tumors was influenced by the nutritional state of the frogs, occurring more readily in well nourished than in poorly nourished animals. Periodic Roentgen ray examinations showed that the size of the primary tumors was not significantly or uniformly affected during the course of the experiments. No correlation was found between change in size of the kidney tumors and the incidence of their metastasis. Although the mechanism by which temperature induces metastasis of frog carcinoma cannot as yet be elucidated, previous experiments with this tumor indicate that certain factors at least may be involved: Elevation of temperature has been found to cause more ready detachment of cells of frog carcinoma in tissue culture; to bring about increased velocity of locomotion of the detached cells; to lead more promptly and efficiently to vascularization of transplants; and to effect their greater invasiveness.

Development and Survivorship of Northern Leopard Frogs (Rana pipiens) and Green Frogs (Rana clamitans) Exposed to Contaminants in the Water and Sediments of the St. Lawrence River near Cornwall, Ontario

2004 ◽  
Vol 39 (3) ◽  
pp. 160-174 ◽  
Author(s):  
Tana V. McDaniel ◽  
Megan L. Harris ◽  
Christine A. Bishop ◽  
John Struger
Keyword(s):  
Rana Pipiens ◽  
Early Stage ◽  
Sediment Quality ◽  
Leopard Frog ◽  
Reference Sites ◽  
Aquatic Life ◽  
Leopard Frogs ◽  
Water And Sediment ◽  
Northern Leopard Frogs ◽  
St Lawrence River

Abstract High levels of contamination in the aquatic environment and wildlife within the Ontario portion of the St. Lawrence River at the Cornwall Area of Concern (AOC) have raised questions about potential impacts on wildlife health. Northern leopard frog embryos were raised in two wetland sites within the AOC and at two reference sites to assess differences in water and sediment quality on survivorship and deformity rates. Chlorinated hydrocarbons (total polychlorinated biphenyls, organochlorine pesticides), polycyclic aromatic hydrocarbons, nutrients and heavy metals were measured in sediment and/or water from the study sites. Levels of some metals such as aluminium, cadmium, chromium and copper, exceeded federal and provincial guidelines for the protection of aquatic life, especially in the two AOC wetlands. Early stage tadpole survivorship was significantly lower and deformity frequency significantly higher at wetlands within the AOC; however, differences were likely not biologically significant. Survivorship and deformity rates of leopard frogs (Rana pipiens) at metamorphosis did not differ significantly among sites. Onset of metamorphosis was accelerated in tadpoles raised in wetlands within the AOC. Tadpoles raised in wetlands within the St. Lawrence River AOC took significantly less time to complete metamorphosis (53–55 days) than did tadpoles raised at reference sites (61–64 days). The phenology of metamorphosis was also more synchronous in tadpoles raised in the AOC, with all tadpoles reaching metamorphosis within a space of 3 to 7 days, as compared to 9 to 12 days at reference wetlands; these differences could not be accounted for by water temperature. Differences in development and survivorship rates between AOC and reference sites may be related to contaminant concentrations in water and sediment. However, no strong evidence for beneficial use impairment in terms of reproductive impairments or elevated deformity rates were seen from caged leopard frogs in the two AOC wetlands.

Nematode parasites and leukocyte profiles of Northern Leopard Frogs, Rana pipiens: location, location, location

2015 ◽  
Vol 93 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Dave Shutler ◽  
Andrée D. Gendron ◽  
Myriam Rondeau ◽  
David J. Marcogliese
Keyword(s):  
Spatial Organization ◽  
Rana Pipiens ◽  
Pesticide Exposure ◽  
Nematode Species ◽  
Agricultural Pesticides ◽  
Leopard Frogs ◽  
Nematode Parasites ◽  
Northern Leopard Frogs ◽  
Host Parasite ◽  
Leukocyte Profiles

Globally, amphibians face a variety of anthropogenic stresses that include exposure to contaminants such as agricultural pesticides. Pesticides may negatively affect amphibian immune systems, concomitantly increasing susceptibility to parasitism. We quantified nematodes and evaluated leukocyte profiles of Northern Leopard Frogs (Rana pipiens Schreber, 1782) collected from five wetlands in southwestern Quebec, Canada, that spanned a gradient of pesticide exposure. Three taxa of nematode parasites (Rhabdias ranae Walton, 1929, genus Oswaldocruzia Travassos, 1917, and genus Strongyloides Grassi, 1879) were sufficiently numerous for detailed evaluation. When all frogs were pooled, frog size was negatively correlated with nematode species richness, abundances of each of the three nematode species, and densities of three different leukocytes. When all frogs were pooled, there was strong evidence of both negative and positive associations between pairs of parasite species. However, none of the previous relationships was significant within wetlands. Our results reveal strong spatial organization of amphibian–parasite communities and illustrate the importance of controlling for sampling locale in evaluating host–parasite associations. Finally, although several response variables varied significantly among wetlands, causes of this variation did not appear to be related to variation in nematode parasitism or pesticide exposure.

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