Comparison of the Genomic Short Regions of a Vaccine Strain (SA-2) and a Virulent Strain (CSW-1) of Infectious Laryngotracheitis Virus (Gallid Herpesvirus 1)

1996 ◽  
Vol 40 (1) ◽  
pp. 130 ◽  
Author(s):  
Halina M. Trist ◽  
Scott G. Tyack ◽  
Michael A. Johnson ◽  
Christopher T. Prideaux ◽  
Michael Sheppard
Keyword(s):  
Vaccine Strain ◽  
Virulent Strain ◽  
Infectious Laryngotracheitis Virus ◽  
Gallid Herpesvirus ◽  
Infectious Laryngotracheitis ◽  
Herpesvirus 1 ◽  
Laryngotracheitis Virus

scholarly journals Multilocus analysis of Gallid herpesvirus 1 in layer chickens in Iraq

2020 ◽  
Vol 13 (1) ◽  
pp. 170-176
Author(s):  
Mohammed Hamzah Abdulkadhim Al-Saadi
Keyword(s):  
Genetic Relatedness ◽  
Polymerase Chain Reaction Method ◽  
Infectious Laryngotracheitis Virus ◽  
Live Attenuated Vaccine ◽  
Gallid Herpesvirus ◽  
Infectious Laryngotracheitis ◽  
Blotting Technique ◽  
Herpesvirus 1 ◽  
Laryngotracheitis Virus ◽  
Chicken Sample

Background and Aim: Infectious laryngotracheitis virus (ILTV) causes a highly pathogenic respiratory disease that affects poultry. It is also known as Gallid herpesvirus 1. ILT prophylaxis measures often include using live attenuated vaccines. The live attenuated vaccine can, however, lead to the formation of new strains of ILTV as a result of vaccine reversion and recombination with field strains. Therefore, this study was performed to explore the multilocus variation of ILTV strains of field and vaccine origin. Samples were tested from two distinctive geographical areas in Iraq as little is known about the ILTV genetic diversity within these areas. Materials and Methods: The polymerase chain reaction method was utilized to generate sequencing templates of six highly polymorphic genes, including UL54, UL52, gB, ICP18.5, ICP4, and gJ in the layer chicken sample (n=15). The Western blotting technique was also employed to detect and estimate the native molecular weight of gE. Results: The results revealed an important degree of genetic relatedness between the field and vaccine strains across all genes. In addition, gE was found to be expressed natively at 49 kDa. Conclusion: The findings of this study may be used to improve the production process of the vaccine for more effective ILT prophylaxis and could further the understanding of epidemiologists and immunologists to better control ILT in the future.

Nucleotide sequence of infectious laryngotracheitis virus (gallid herpesvirus 1) ICP4 gene

1995 ◽  
Vol 35 (2) ◽  
pp. 193-204 ◽  
Author(s):  
Michael A. Johnson ◽  
Scott G. Tyack ◽  
Christopher Prideaux ◽  
Kritaya Kongsuwan ◽  
Michael Sheppard
Keyword(s):  
Nucleotide Sequence ◽  
Infectious Laryngotracheitis Virus ◽  
Gallid Herpesvirus ◽  
Infectious Laryngotracheitis ◽  
Herpesvirus 1 ◽  
Laryngotracheitis Virus

Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain

1991 ◽  
Vol 119 (3-4) ◽  
pp. 181-198 ◽  
Author(s):  
M. A. Johnson ◽  
C. T. Prideaux ◽  
K. Kongsuwan ◽  
M. Sheppard ◽  
K. J. Fahey
Keyword(s):  
Infectious Laryngotracheitis Virus ◽  
Gallid Herpesvirus ◽  
Physical Maps ◽  
Infectious Laryngotracheitis ◽  
Herpesvirus 1 ◽  
Laryngotracheitis Virus

scholarly journals Psittacid Herpesvirus 1 and Infectious Laryngotracheitis Virus: Comparative Genome Sequence Analysis of Two Avian Alphaherpesviruses

2006 ◽  
Vol 80 (16) ◽  
pp. 7863-7872 ◽  
Author(s):  
Dean R. Thureen ◽  
Calvin L. Keeler
Keyword(s):  
Genome Sequence ◽  
Pseudorabies Virus ◽  
Structural Characteristics ◽  
Open Reading Frames ◽  
Published Data ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Herpesvirus 1 ◽  
Laryngotracheitis Virus

ABSTRACT Psittacid herpesvirus 1 (PsHV-1) is the causative agent of Pacheco's disease, an acute, highly contagious, and potentially lethal respiratory herpesvirus infection in psittacine birds, while infectious laryngotracheitis virus (ILTV) is a highly contagious and economically significant avian herpesvirus which is responsible for an acute respiratory disease limited to galliform birds. The complete genome sequence of PsHV-1 has been determined and compared to the ILTV sequence, assembled from published data. The PsHV-1 and ILTV genomes exhibit similar structural characteristics and are 163,025 bp and 148,665 bp in length, respectively. The PsHV-1 genome contains 73 predicted open reading frames (ORFs), while the ILTV genome contains 77 predicted ORFs. Both genomes contain an inversion in the unique long region similar to that observed in pseudorabies virus. PsHV-1 is closely related to ILTV, and it is proposed that it be assigned to the Iltovirus genus. These two avian herpesviruses represent a phylogenetically unique clade of alphaherpesviruses that are distinct from the Marek's disease-like viruses (Mardivirus). The determination of the complete genomic nucleotide sequences of PsHV-1 and ILTV provides a tool for further comparative and functional analysis of this unique class of avian alphaherpesviruses.

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