In vivo Evaluation of the Pathogenicity of Field Isolates of Infectious Bronchitis Virus

1994 ◽  
Vol 38 (3) ◽  
pp. 589 ◽  
Author(s):  
G. E. Avellaneda ◽  
P. Villegas ◽  
M. W. Jackwood ◽  
D. J. King
Keyword(s):  
Infectious Bronchitis Virus ◽  
In Vivo Evaluation ◽  
Infectious Bronchitis ◽  
Field Isolates

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

scholarly journals Molecular and Antigenic Characterization of GI-13 and GI-16 Avian Infectious Bronchitis Virus Isolated in Chile from 2009 to 2017 Regarding 4/91 Vaccine Introduction

Animals ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 656 ◽  
Author(s):  
Miguel Guzmán ◽  
Leonardo Sáenz ◽  
Héctor Hidalgo
Keyword(s):  
Infectious Bronchitis Virus ◽  
Vaccination Programs ◽  
Infectious Bronchitis ◽  
Field Isolates ◽  
Vaccine Introduction ◽  
Antigenic Characterization ◽  
Antigenic Relatedness ◽  
Likelihood Approach ◽  
Phylogenetic Lineages

The introduction of the 4/91 vaccine against infectious bronchitis in Chile, a lineage not described until that time in the country, led to looking for changes induced by this action. This study considers eight isolates obtained from 2009, 2015 and 2017 and uses a maximum likelihood approach to classify the field isolates. Three isolates were selected to analyze antigenic relationships through a virus neutralization test and to perform protection tests measured trough an RT-qPCR. The isolates from 2009 and 2015 showed a relationship with GI-16 while those from 2017 were related to GI-13. Though the field isolates were classified in two different phylogenetic lineages, all of them showed only minor variations in subtype. The 13885R-17 isolate from 2017 exhibited high antigenic relatedness to the 4/91 vaccine. As expected, 4/91 and Massachusetts vaccines were not antigenically related. Vaccinated birds with the 4/91 vaccine showed less tracheal virus replication for the 13885R-17 from 2017 challenge than for the 12101SP-09 from 2009 and 13347SP-15 from 2015 isolates. The results indicated genetic and antigenic diversity in the most recent infectious bronchitis virus (IBV) isolates in Chile. Moreover, the 4/91 vaccine would be involved in the generation of some current field viruses, which must be considered in vaccination programs and public policies.

Appearance of Haemagglutinability of Infectious Bronchitis Virus Following In Vitro and In Vivo Tracheal Passages

2010 ◽  
Vol 32 (1-10) ◽  
pp. 736-743
Author(s):  
Shunsuke Yachida ◽  
Kazunari Sawaguchi ◽  
Shigemi Aoyama ◽  
Yoshikazu Iritani ◽  
Yoshiyuki Hayashi
Keyword(s):  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals Avian coronavirus infectious bronchitis virus susceptibility to botanical oleoresins and essential oils in vitro and in vivo

2010 ◽  
Vol 149 (1) ◽  
pp. 86-94 ◽  
Author(s):  
M.W. Jackwood ◽  
R. Rosenbloom ◽  
M. Petteruti ◽  
D.A. Hilt ◽  
A.W. McCall ◽  
...  
Keyword(s):  
Essential Oils ◽  
Infectious Bronchitis Virus ◽  
Virus Susceptibility ◽  
Infectious Bronchitis

Molecular and Serologic Characterization, Pathogenicity, and Protection Studies with Infectious Bronchitis Virus Field Isolates from California

2007 ◽  
Vol 51 (2) ◽  
pp. 527-533 ◽  
Author(s):  
Mark W. Jackwood ◽  
Deborah A. Hilt ◽  
Susan M. Williams ◽  
Peter Woolcock ◽  
Carol Cardona ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Field Isolates
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