Propagation of Avian Rotavirus in Primary Chick Kidney Cell and MA104 Cell Cultures

1989 ◽  
Vol 33 (3) ◽  
pp. 578 ◽  
Author(s):  
T. J. Myers ◽  
K. A. Schat
Keyword(s):  
Cell Cultures ◽  
Kidney Cell ◽  
Ma104 Cell ◽  
Chick Kidney

scholarly journals Effect of oestrogen and 1,25-dihydroxycholecalciferol on 25-hydroxycholecalciferol metabolism in primary chick kidney-cell cultures

1978 ◽  
Vol 174 (1) ◽  
pp. 231-236 ◽  
Author(s):  
E Spanos ◽  
D I Barrett ◽  
K T Chong ◽  
I MacIntyre
Keyword(s):  
Vitamin D ◽  
Cell Cultures ◽  
Kidney Cell ◽  
Primary Cultures ◽  
Kidney Cells ◽  
Hydroxylase Activity ◽  
Experimental Conditions ◽  
Polar Metabolites ◽  
Chick Kidney ◽  
25 Hydroxycholecalciferol

Primary cultures of chick kidney cells convert 25-hydroxycholecalciferol into more-polar metabolites. Cells from vitamin D-deficient chicks have high 25-hydroxycholecalciferol 1 alpha-hydroxylase (1 alpha-hydroxylase) activity, but no 25-hydroxycholecalciferol 24-hydroxylase (24-hydroxylase) activity. Physiological concentrations of 1,25-dihydroxycholeclaciferol suppress 1 alpha-hydroxylase and induce 24-hydroxylase activity. The inhibition of 1 alpha-hydroxylase preceded the induction of 24-hydroxylase. In contrast, oestradiol-17 beta had no effect on the activity of either hydroxylase under a variety of experimental conditions. These results clearly demonstrate that 1,25-dihydroxycholecalciferol, but not oestrogen, acts directly on the kidney cells to regulate the metabolism of 25-hydroxycholecalciferol.

25(OH)D3 metabolism in kidney cell cultures: lack of a direct effect of estradiol

1981 ◽  
Vol 240 (2) ◽  
pp. E119-E124 ◽  
Author(s):  
H. L. Henry
Keyword(s):  
Direct Effect ◽  
Cell Cultures ◽  
Kidney Cell ◽  
Renal Cell ◽  
Primary Cultures ◽  
Kidney Cells ◽  
Hydroxylase Activity ◽  
Dihydroxyvitamin D3 ◽  
Chick Kidney

There are several reports of increased production of 1 alpha,25-dihydroxyvitamin D3 [1,25(OH)2D3] by the kidney of birds in response to estrogen treatment. To determine whether estradiol influences the renal cell directly, primary cultures of chick kidney cells were exposed to the steroid under a variety of conditions. In the absence of 1,25(OH)2D3, treatment of cultures for 20-24 h with 10(-5) and 10(-6) M estradiol led to inhibition of 25-hydroxyvitamin D3 [25(OH)D3]-1-hydroxylase activity. When the 1-hydroxylase was suppressed and 25(OH)2D3-24-hydroxylase was induced by treatment with 1,25(OH)2D3, estradiol in concentrations of 10(-9) and 10(-5) M either had no effect or slightly inhibited 1,25(OH)2D3 production. Similarly, 24R,25-dihydroxyvitamin D3[24,25(OH)2D3] production was not affected consistently by estradiol. These results were unaltered when either testosterone (10(-6) M) or insulin (5 micrograms/ml) was present in the medium. Shorter treatments (0.5, 2, 4, and 8 h) with estradiol resulted in a transient decrease in both 1,25(OH)2D3 and 24,25(OH)2D3 production, but at no time was stimulation observed. These results suggest that the effects of estrogens on 25(OH)D3 metabolism observed in vivo are exerted elsewhere than directly at the renal cell.

scholarly journals Regulation of 25-OHD3 metabolism by parathyroid hormone in primary chick kidney cell cultures

FEBS Letters ◽  
1979 ◽  
Vol 105 (1) ◽  
pp. 31-34 ◽  
Author(s):  
E. Spanos ◽  
D.J. Brown ◽  
I. MacIntyre
Keyword(s):  
Parathyroid Hormone ◽  
Cell Cultures ◽  
Kidney Cell ◽  
Chick Kidney

Regulation of 25-OHD3 Metabolism in Isolated Chick Kidney Cell Cultures

1978 ◽  
Vol 55 (3) ◽  
pp. 8P-8P
Author(s):  
Penelope Raptis ◽  
D. I. Barrett ◽  
D. J. Brown ◽  
I. MacIntyre ◽  
E. Spanos
Keyword(s):  
Cell Cultures ◽  
Kidney Cell ◽  
Chick Kidney
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