Isolation and Serial Propagation of Turkey Rotaviruses in a Fetal Rhesus Monkey Kidney (MA104) Cell Line

1986 ◽  
Vol 30 (1) ◽  
pp. 93 ◽  
Author(s):  
Kenneth W. Theil ◽  
Donald L. Reynolds ◽  
Yehia M. Saif
Keyword(s):  
Rhesus Monkey ◽  
Cell Line ◽  
Monkey Kidney ◽  
Ma104 Cell ◽  
Fetal Rhesus Monkey Kidney ◽  
Serial Propagation

Primary isolation of cytopathic bovine rotaviruses on fetal rhesus monkey kidney cells

1983 ◽  
Vol 8 (2) ◽  
pp. 135-139 ◽  
Author(s):  
Yosuke Murakami ◽  
Nobuyoshi Nishioka ◽  
Yuji Hashiguchi ◽  
Chikara Kuniyasu
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Primary Isolation ◽  
Fetal Rhesus Monkey Kidney

scholarly journals Susceptibility of the LLC-MK2 line of monkey kidney cells to human enteroviruses

1965 ◽  
Vol 63 (2) ◽  
pp. 169-174 ◽  
Author(s):  
M. H. Hambling ◽  
Patricia M. Davis
Keyword(s):  
Rhesus Monkey ◽  
Cell Line ◽  
Monkey Kidney ◽  
Continuous Cell Line ◽  
Kidney Cells ◽  
Relative Susceptibility ◽  
Coxsackie B

The relative susceptibility of the LLC-MK2 cell line and primary rhesus monkey kidney cultures to thirty-eight prototype strains of human enteroviruses is described; of these strains only ECHO types 16, 18, 21 and 23 failed to cause CPE in the continuous cell line. The efficiency of the two tissues for the isolation of enteroviruses from faecal extracts is compared. The results show that the LLC-MK2 cell line is very satisfactory for the isolation of Coxsackie B and polioviruses, but not so useful for the isolation of ECHO viruses. The identification of enteroviruses by neutralization tests in LLC-MK2 cells is successful.

scholarly journals Enterovirus 70 Receptor Utilization Is Controlled by Capsid Residues That Also Regulate Host Range and Cytopathogenicity

2007 ◽  
Vol 81 (16) ◽  
pp. 8648-8655 ◽  
Author(s):  
Melissa Stewart Kim ◽  
Vincent R. Racaniello
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rhesus Monkey ◽  
Host Range ◽  
Hela Cells ◽  
Lytic Replication ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Cytopathic Effects ◽  
Axis Of Symmetry

ABSTRACT Enterovirus type 70, an etiologic agent of acute hemorrhagic conjunctivitis, may bind different cellular receptors depending on cell type. To understand how EV70-receptor interaction is controlled, we studied two variants of the virus with distinct receptor utilization. EV70-Rmk, derived by passage in rhesus monkey kidney cells, replicates poorly in HeLa cells and does not cause cytopathic effects. Decay accelerating factor (DAF) is not a cell receptor for EV70-Rmk. Passage of EV70-Rmk in HeLa cells lead to isolation of EV70-Dne, which does not replicate in rhesus monkey kidney cells but grows to high titers in HeLa cells and causes cytopathic effects. DAF is sufficient for cell entry of EV70-Dne. EV70-Rmk replicates in human eye and brain-derived cell lines, whereas the Dne strain replicates only in HeLa cells and in conjunctiva-derived 15C4 cells. The two EV70 strains differ by five amino acid changes in the viral capsid. Single substitution of four of the five EV70-Rmk amino acids with the residue from EV70-Dne leads to lytic replication in HeLa cells. Conversely, substitution of any of the five EV70-Dne amino acids with the EV70-Rmk amino acid does not alter replication in HeLa cells. Three of these capsid amino acids are predicted to be located in the canyon encircling the fivefold axis of symmetry, one amino acid is found at the fivefold axis of symmetry, and one is located the interior of the capsid. The five EV70 residues define a region of the capsid that controls viral host range, DAF utilization, and cytopathogenicity.

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