Tracheal Populations of Mycoplasma gallisepticum after Challenge of Bacterin-Vaccinated Chickens

1985 ◽  
Vol 29 (4) ◽  
pp. 1012 ◽  
Author(s):  
S. H. Kleven
Keyword(s):  
Mycoplasma Gallisepticum

scholarly journals GAA Trinucleotide Repeat Region Regulates M9/pMGA Gene Expression in Mycoplasma gallisepticum

2000 ◽  
Vol 68 (2) ◽  
pp. 871-876 ◽  
Author(s):  
Li Liu ◽  
Kevin Dybvig ◽  
Victor S. Panangala ◽  
Vicky L. van Santen ◽  
Christopher T. French
Keyword(s):  
Gene Expression ◽  
Trinucleotide Repeat ◽  
Mycoplasma Gallisepticum ◽  
Avian Host ◽  
Phase Variable ◽  
Repeat Region ◽  
Chromosomal Dna ◽  
Promoter Regions ◽  
Variable Expression ◽  
Gaa Repeats

ABSTRACT Mycoplasma gallisepticum, the cause of chronic respiratory infections in the avian host, possesses a family of M9/pMGA genes encoding an adhesin(s) associated with hemagglutination. Nucleotide sequences of M9/pMGA gene family members indicate extensive sequence similarity in the promoter regions of both the transcribed and silent genes. The mechanism that regulates M9/pMGA gene expression is unknown, but studies have revealed an apparent correlation between gene expression and the number of tandem GAA repeat motifs located upstream of the putative promoter. In this study, transposon Tn4001was used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of the GAA repeats in M9/pMGA gene expression in M. gallisepticum. A 336-bp M9 gene fragment (containing the GAA repeat region, the promoter, and the translation start codon) was amplified by PCR, ligated with alacZ gene from E. coli, and inserted into the Tn4001-containing plasmid pISM2062. This construct was transformed into M. gallisepticum PG31. Transformants were filter cloned on agar supplemented with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color selection. Several cycles of filter cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off states in successive generations of progeny clones. The promoter regions of the M9-lacZ hybrid genes of individual progeny clones were amplified by PCR and sequenced. The only differences between the promoter regions of the blue and white colonies were in the number of GAA repeats. Clones that expressedlacZ had exactly 12 tandem copies of the GAA repeat. Clones that did not express lacZ invariably had either more than 12 (14 to 16) or fewer than 12 (5 to 11) GAA repeats. Southern analysis of M. gallisepticum chromosomal DNA confirmed that the phase-variable expression of the lacZ reporter gene was not caused by Tn4001 transposition. These data strongly indicate that changes in the length of the GAA repeat region are responsible for regulating M9/pMGA gene expression.

scholarly journals Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: Morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium

2010 ◽  
Vol 165 (4) ◽  
pp. 346-350 ◽  
Author(s):  
Vladislav M. Chernov ◽  
Olga A. Chernova ◽  
Alexey A. Mouzykantov ◽  
Anastasia A. Ponomareva ◽  
Maxim V. Trushin ◽  
...  
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Viable But Nonculturable

Effects of an S6 Strain of Mycoplasma gallisepticum Challenge Before Beginning of Lay on Various Egg Characteristics in Commercial Layers

2002 ◽  
Vol 46 (3) ◽  
pp. 593-597 ◽  
Author(s):  
T. A. Parker ◽  
S. L. Branton ◽  
M. S. Jones ◽  
E. D. Peebles ◽  
P. D. Gerard ◽  
...  
Keyword(s):  
Mycoplasma Gallisepticum

Mycoplasma gallisepticum modifies the pathogenesis of influenza A virus in the avian tracheal epithelium

2016 ◽  
Vol 306 (3) ◽  
pp. 174-186 ◽  
Author(s):  
Hicham Sid ◽  
Sandra Hartmann ◽  
Henning Petersen ◽  
Martin Ryll ◽  
Silke Rautenschlein
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Mycoplasma Gallisepticum ◽  
Tracheal Epithelium

Effects of immunosuppression on the efficacy of vaccination against Mycoplasma gallisepticum infection in chickens

2021 ◽  
pp. 109182
Author(s):  
Sathya N. Kulappu Arachchige ◽  
Anna Kanci Condello ◽  
Ling Zhu ◽  
Pollob K. Shil ◽  
Kelly A. Tivendale ◽  
...  
Keyword(s):  
Mycoplasma Gallisepticum
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