Antigenic Properties of Four Serotypes of Pasteurella multocida Determined by Enzyme-Linked Immunosorbent Assay

1986 ◽  
Vol 30 (3) ◽  
pp. 477 ◽  
Author(s):  
A. P. Avakian ◽  
J. W. Dick
Keyword(s):  
Immunosorbent Assay ◽  
Pasteurella Multocida ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigenic Properties

Comparison of indirect haemagglutination test, gel-diffusion precipitin test, and enzyme-linked immunosorbent assay for detection of serum antibodies to Pasteurella multocida in naturally and experimentally infected rabbits

1994 ◽  
Vol 28 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Eiichi Kawamoto ◽  
Takuo Sawada ◽  
Toru Sato ◽  
Kiyoshi Suzuki ◽  
Tsutomu Maruyama
Keyword(s):  
Immunosorbent Assay ◽  
Pasteurella Multocida ◽  
Rabbit Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Inoculation ◽  
Serum Samples ◽  
Serological Tests ◽  
Indirect Haemagglutination ◽  
Precipitin Test ◽  
Gel Diffusion

Enzyme-linked immunosorbent assay (ELISA), gel-diffusion precipitin test (GDPT), and indirect haem agglutination test (IHAT) were evaluated for the detection of antibodies to Pasteurella multocida in both naturally and experimentally infected rabbits. A total of 285 rabbit serum samples from 7 rabbit colonies were tested by ELISA, GDPT, and IHAT, and nasal cultures were taken coincidentally to use as the standard in the serological tests. There was better correlation (98.0%) between the results of ELISA and positive nasal culture than between the GDPT (86.3%) or IHAT (23.5%) and positive nasal culture. In addition, ELISA and GDPT were positive in 26 (11.1%) and 21 (9.0%) of 234 serum samples from nasal culture negative rabbits, respectively. In experimentally infected rabbits, antibodies detected by the ELISA and GDPT began to rise one to 3 weeks post-inoculation. IHAT did not detect antibodies. These results are discussed in terms of value to serodiagnosis of rabbit pasteurellosis.

scholarly journals Obtaining Recombinant Antigens for the Development of Serological Diagnosis of Marburg Fever

2021 ◽  
pp. 47-52
Author(s):  
N. V. Volkova ◽  
A. V. Ivanova ◽  
A. A. Isaeva ◽  
O. A. Polezhaeva ◽  
A. V. Zaykovskaya ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Immunosorbent Assay ◽  
Guinea Pigs ◽  
Matrix Protein ◽  
High Titer ◽  
Culture Fluid ◽  
Marburg Virus ◽  
Surface Glycoprotein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigenic Properties

Aim. Production of recombinant viral antigens of the main immunodominant proteins: glycoprotein (GPΔMLD), nucleoprotein (NP) and matrix protein (VP40) of the Marburg virus, as well as the study of their antigenic and immunogenic properties.Materials and methods. To create recombinant proteins GPΔMLD, NP and VP40 of the Marburg virus, synthesized nucleotide sequences encoding these proteins cloned into the pET21a expression vector were used. The immunogenic and antigenic properties of the obtained recombinant proteins were tested using a number of biomodels (mice, chickens, and guinea pigs).Results and discussion. Recombinant plasmids containing genes encoding proteins GPΔMLD, NP, VP40 of the Marburg virus, as well as Escherichia coli producing strains, with the yield of purified preparations of recombinant proteins GPΔMLD, NP, VP40 from one liter of culture fluid – 5, 10, and 10 μg were obtained, respectively. When mice are immunized, recombinant proteins GP, NP, and VP40 MARV induce the synthesis of high titer antibodies (recombinant proteins NP and VP40 – more than 409600, and recombinant protein GPΔMLD – 12800). Mouse antibodies specific to recombinant proteins interact in an enzyme-linked immunosorbent assay (ELISA) with the antigen of inactivated MARV. Antibodies of chickens immunized with virus-like particles containing the surface glycoprotein of the Marburg virus and antibodies of guinea pigs immunized with an experimental DNA vaccine containing the GPΔMLD MARV gene recognize the recombinant GPΔMLD protein and the viral protein in the inactivated MARV. The resulting recombinant proteins are immunogenic/antigenic and can be used for the development of enzymelinked immunosorbent assay systems. 

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