Pathogenicity of Infectious Laryngotracheitis Virus as Measured by Chicken Embryo Inoculation

1982 ◽  
Vol 26 (1) ◽  
pp. 18 ◽  
Author(s):  
Toshiro Izuchi ◽  
Akira Hasegawa
Keyword(s):  
Chicken Embryo ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus

scholarly journals Pathogenic and Transmission Potential of Wildtype and Chicken Embryo Origin (CEO) Vaccine Revertant Infectious Laryngotracheitis Virus

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 541
Author(s):  
Ana Perez-Contreras ◽  
Catalina Barboza-Solis ◽  
Shahnas M. Najimudeen ◽  
Sylvia Checkley ◽  
Frank van der Meer ◽  
...  
Keyword(s):  
Chicken Embryo ◽  
Severe Disease ◽  
Clinical Signs ◽  
Upper Respiratory Tract ◽  
Infectious Laryngotracheitis Virus ◽  
Live Attenuated Vaccines ◽  
Transmission Potential ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
Embryo Origin

Infectious laryngotracheitis (ILT) is an infectious upper respiratory tract disease that impacts the poultry industry worldwide. ILT is caused by an alphaherpesvirus commonly referred to as infectious laryngotracheitis virus (ILTV). Vaccination with live attenuated vaccines is practiced regularly for the control of ILT. However, extensive and improper use of live attenuated vaccines is related to vaccine viruses reverting to virulence. An increase in mortality and pathogenicity has been attributed to these vaccine revertant viruses. Recent studies characterized Canadian ILTV strains originating from ILT outbreaks as related to live attenuated vaccine virus revertants. However, information is scarce on the pathogenicity and transmission potential of these Canadian isolates. Hence, in this study, the pathogenicity and transmission potential of two wildtype ILTVs and a chicken embryo origin (CEO) vaccine revertant ILTV of Canadian origin were evaluated. To this end, 3-week-old specific pathogen-free chickens were experimentally infected with each of the ILTV isolates and compared to uninfected controls. Additionally, naïve chickens were exposed to the experimentally infected chickens to mimic naturally occurring infection. Pathogenicity of each of these ILTV isolates was evaluated by the severity of clinical signs, weight loss, mortality, and lesions observed at the necropsy. The transmission potential was evaluated by quantification of ILTV genome loads in oropharyngeal and cloacal swabs and tissue samples of the experimentally infected and contact-exposed chickens, as well as in the capacity to produce ILT in contact-exposed chickens. We observed that the CEO vaccine revertant ILTV isolate induced severe disease in comparison to the two wildtype ILTV isolates used in this study. According to ILTV genome load data, CEO vaccine revertant ILTV isolate was successfully transmitted to naïve contact-exposed chickens in comparison to the tested wildtype ILTV isolates. Overall, the Canadian origin CEO vaccine revertant ILTV isolate possesses higher virulence, and dissemination potential, when compared to the wildtype ILTV isolates used in this study. These findings have serious implications in ILT control in chickens.

Chicken embryo origin-like strains are responsible for Infectious laryngotracheitis virus outbreaks in Egyptian cross-bred broiler chickens

Virus Genes ◽  
2013 ◽  
Vol 46 (3) ◽  
pp. 423-430 ◽  
Author(s):  
Awad A. Shehata ◽  
Mohammad Y. Halami ◽  
Hesham H. Sultan ◽  
Alaa G. Abd El-Razik ◽  
Thomas W. Vahlenkamp
Keyword(s):  
Chicken Embryo ◽  
Broiler Chickens ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
Embryo Origin

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Sign in / Sign up

Export Citation Format

Share Document