Slide-Agglutination Test of Haemophilus gallinarum Antigen Treated by Trypsin to Inhibit Spontaneous Agglutination

1978 ◽  
Vol 22 (4) ◽  
pp. 793
Author(s):  
Yoshikazu Iritani ◽  
Ken Katagiri ◽  
Keiko Tsuji
Keyword(s):  
Agglutination Test ◽  
Slide Agglutination ◽  
Slide Agglutination Test

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals A slide agglutination test for the exclusion and diagnosis of typhoid fever

1939 ◽  
Vol 39 (3) ◽  
pp. 294-297 ◽  
Author(s):  
F. M. Berger ◽  
G. Brecher
Keyword(s):  
Typhoid Fever ◽  
Positive Result ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Agglutination Reaction ◽  
Negative Test ◽  
Slide Agglutination ◽  
Peculiar Behaviour ◽  
Slide Agglutination Test ◽  
Agglutination Method

A sensitive antigen suspension is described for use with a simple slide agglutination method which makes possible a serological diagnosis or exclusion of typhoid fever without recourse to a laboratory. The method has been tested on 414 sera sent to our laboratory; it detected all cases with a titre of 1: 80 or more, and most of those with a titre of 1: 40. The method was further tested on 130 clinically observed cases, in which it gave satisfactory results. The S. A. method gave a positive result with 98 out of 100 sera from patients with typhoid fever, whereas the classical Widal reaction gave a positive result with 68 of them only.The intensity and rapidity of the slide agglutination reaction provide a rough measure of the titre of a serum. A quick and distinct agglutination indicates a titre of 1: 80 or more and is diagnostic of typhoid fever. A slow and indistinct result is obtained when the titre of the serum is about 1: 40. A negative test indicates with great probablity that a diagnosis of typhoid fever may be excluded.We think the method succeeds because the nature of the suspension employed and the peculiar behaviour of slide agglutinations permit the detection of O agglutinins as well as H agglutinins.

Utilization of a real-time PCR approach for Haemophilus influenzae serotype determination as an alternative to the slide agglutination test

2013 ◽  
Vol 27 (2) ◽  
pp. 86-89 ◽  
Author(s):  
Danielle Wroblewski ◽  
Tanya A. Halse ◽  
Jill Hayes ◽  
Donna Kohlerschmidt ◽  
Kimberlee A. Musser
Keyword(s):  
Haemophilus Influenzae ◽  
Real Time ◽  
Real Time Pcr ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Slide Agglutination Test

A new rapid slide agglutination test for amniotic fluid phosphatidylglycerol: Laboratory and clinical correlation

1983 ◽  
Vol 147 (6) ◽  
pp. 681-686 ◽  
Author(s):  
Thomas J. Garite ◽  
Kenichi K. Yabusaki ◽  
Leslie J. Moberg ◽  
Judy L. Symons ◽  
Terry White ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Agglutination Test ◽  
Clinical Correlation ◽  
Slide Agglutination ◽  
Slide Agglutination Test

Specificity of slide agglutination test for detecting bacterial fish pathogens

Aquaculture ◽  
1987 ◽  
Vol 61 (2) ◽  
pp. 81-97 ◽  
Author(s):  
Alicia E. Toranzo ◽  
Ana M. Baya ◽  
Bob S. Roberson ◽  
Juan L. Barja ◽  
D.Jay Grimes ◽  
...  
Keyword(s):  
Agglutination Test ◽  
Fish Pathogens ◽  
Slide Agglutination ◽  
Slide Agglutination Test

scholarly journals Evaluation of the Performance of Widal Slide Agglutination Test Compared to Blood Culture and Evaluation of Interferon Gamma Response in the Diagnosis of Typhoid Fever

2020 ◽  
pp. 117-122
Author(s):  
Mujahid N.S. ◽  
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Interferon Gamma ◽  
Agglutination Test ◽  
Antibody Detection ◽  
Widal Test ◽  
Blood Samples ◽  
Slide Agglutination ◽  
Slide Agglutination Test ◽  
Interferon Gamma Response

Typhoid fever remains a public health challenge in developing countries including Nigeria. Widal test is widely used for the diagnosis of typhoid fever due to its simplicity and short turnaround time. However, the specificity of this test has been debated. The aim of the study was to evaluate the performance of Widal test compared to blood culture and determine interferon gamma response among the study subjects. Blood samples were collected from 90 patients who complained of fever and other symptoms suggestive of typhoid fever. Widal slide agglutination test, automated blood culture and interferon gamma concentrations were conducted using rapid antibody detection kit, BACTEC and sandwich enzyme linked immunosorbent assay (ELISA) respectively. Of the 90 samples tested, 63 (70.0%) were positive for anti-Typhi O antigen while 42 (46.7%) were positive for anti-Typhi H antigen. Similarly, 18 (20%) of the blood samples were non- S. Typhi culture positive while 72 (80%) had no bacteria isolated. None of the cases had S. Typhi positive culture. With regards to interferon gamma, subjects with lower levels of 15pg/mL had no bacteria isolated from their blood. As the interferon gamma concentration increased, more subjects had non- S. Typhi bacteria isolated from their blood which shows the relationship between interferon gamma and bacteraemia. The study demonstrated that the use of Widal serology test in the diagnosis of typhoid fever may be erroneous as all the samples were found to be negative for S. Typhi using the gold standard culture methods while Interferon gamma concentration was statistically related to the isolation of non- S. Typhi in blood culture as such, could be a good marker for the development of an alternative screening test, possibly an interferon gamma based detection system for typhoid fever. However, further research is recommended to elucidate that. Keywords: Typhoid, Widal test, Blood culture, Interferon gamma

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